| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92339-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92202-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35880-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52556-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50300-0.025ml | |||
![Western Blot: ABCA2 Antibody [NBP2-92339] - Analysis of extracts of various cell lines, using ABCA2 .Exposure time: 3min.](http://images.novusbio.com/fullsize/ABCA2-Antibody-Western-Blot-NBP2-92339-img0003.jpg "Western Blot: ABCA2 Antibody [NBP2-92339] - Analysis of extracts of various cell lines, using ABCA2 .Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: ABCA2 Antibody - Azide and BSA Free [NBP2-92339] - Immunofluorescence analysis of paraffin-embedded mouse brain using ABCA2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92339_rabbit-polyclonal-abca2-antibody-4220251429023.jpg%3Ft=1709177288)
![Immunocytochemistry/ Immunofluorescence: ABCA2 Antibody - Azide and BSA Free [NBP2-92339] - Immunofluorescence analysis of U-251 MG cells using ABCA2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92339_rabbit-polyclonal-abca2-antibody-42202514264481.jpg%3Ft=1709177288)
![Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Western-Blot-NBP2-92202-img0002.jpg "Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ACSS1 Antibody [NBP2-92202] - Analysis of L929 cells using ACSS1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92202-img0001.jpg)
![Immunohistochemistry-Paraffin: ACSS1 Antibody [NBP2-92202] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACSS1 Rabbit pAb (NBP2-92202) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-92202-img0003.jpg)
![Western Blot: SKIV2L Antibody [NBP2-81903] - Analysis of SKIV2L in mouse stomach tissue lysate with SKIV2L antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/SKIV2L-Antibody-Western-Blot-NBP2-81903-img0001.jpg "Western Blot: SKIV2L Antibody [NBP2-81903] - Analysis of SKIV2L in mouse stomach tissue lysate with SKIV2L antibody at 1 ug/ml.")
![Immunohistochemistry: SKIV2L Antibody - BSA Free [NBP2-81903] - Immunohistochemistry of SKIV2L in human stomach tissue with SKIV2L antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81903_rabbit-polyclonal-skiv2l-antibody-2711202419531670.jpg)
![Immunocytochemistry/ Immunofluorescence: SKIV2L Antibody - BSA Free [NBP2-81903] - Immunofluorescence of SKIV2L in human stomach tissue with SKIV2L antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81903_rabbit-polyclonal-skiv2l-antibody-2711202420333860.jpg)
![Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-9I9J8-Western-Blot-NBP3-15615-img0017.jpg "Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Human colon using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410253..jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] -Human lung using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410355..jpg)
![Western Blot: Caveolin-1 Antibody (10Y5L2) [Caveolin-1] - Western blot analysis of various lysates, using Caveolin-1 Rabbit mAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-5220251731106.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (7Z9H0) [NBP3-16525] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 alpha Rabbit mAb (NBP3-16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-7Z9H0-Western-Blot-NBP3-16525-img0002.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (7Z9H0) [NBP3-16525] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 alpha Rabbit mAb (NBP3-16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: PI 3-Kinase p85 alpha Antibody (3X3T8) [NBP3-16525] - Western blot analysis of various lysates, using PI 3-Kinase p85 alpha Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16525_rabbit-pi-3-kinase-p85-alpha-mab-3x3t8-52202511365130.jpg)
![Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase Antibody [NBP3-35880] - Immunofluorescence analysis of paraffin-embedded Mouse liver using alcohol dehydrogenase Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35880_rabbit-alcohol-dehydrogenase-pab-101220241252870.jpg "Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase Antibody [NBP3-35880] - Immunofluorescence analysis of paraffin-embedded Mouse liver using alcohol dehydrogenase Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: alcohol dehydrogenase Antibody [NBP3-35880] - Western blot analysis of lysates from Mouse lung, using alcohol dehydrogenase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35880_rabbit-alcohol-dehydrogenase-pab-1012202411535849.jpg)
![Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase Antibody [NBP3-35880] - Immunofluorescence analysis of paraffin-embedded Rat liver using alcohol dehydrogenase Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35880_rabbit-alcohol-dehydrogenase-pab-101220241243956.jpg)
![Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase Antibody [NBP3-35880] - Immunofluorescence analysis of paraffin-embedded Human liver using alcohol dehydrogenase Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35880_rabbit-alcohol-dehydrogenase-pab-10122024121066.jpg)
![Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of extracts of various cell lines, using PLTP antibody (NBP3-16847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Western-Blot-NBP3-16847-img0003.jpg "Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of extracts of various cell lines, using PLTP antibody (NBP3-16847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: PLTP Antibody (6A1Q1) [NBP3-16847] - Immunohistochemistry of paraffin-embedded mouse testis using PLTP Rabbit mAb (NBP3-16847) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Immunohistochemistry-Paraffin-NBP3-16847-img0002.jpg)
![Immunohistochemistry-Paraffin: PLTP Antibody (6A1Q1) [NBP3-16847] - Immunohistochemistry of paraffin-embedded rat pancreas using PLTP Rabbit mAb (NBP3-16847) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Immunohistochemistry-Paraffin-NBP3-16847-img0001.jpg)
![Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of various lysates using PLTP Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16847_rabbit-pltp-mab-6a1q1-52202511361237.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0003.jpg "Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0007.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0006.jpg)
![ELISA: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-ELISA-NBP2-52556-img0005.jpg)
![Western Blot: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3.](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Western-Blot-NBP2-71525-img0001.jpg "Western Blot: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3.")
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0004.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0002.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0003.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0005.jpg)
![Western Blot: Myeloperoxidase/MPO Antibody (A1F2-R) [NBP3-32623] - Western blot analysis of Myeloperoxidase/MPO on HL-60 cell lysates with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84/55/37 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32623) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32623_mouse-myeloperoxidase-mpo-mab-a1f2-r-27820241631665.jpg "Western Blot: Myeloperoxidase/MPO Antibody (A1F2-R) [NBP3-32623] - Western blot analysis of Myeloperoxidase/MPO on HL-60 cell lysates with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84/55/37 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32623) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Myeloperoxidase/MPO Antibody (A1F2-R) [NBP3-32623] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32623) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32623_mouse-myeloperoxidase-mpo-mab-a1f2-r-29820241492616.jpg)
![Immunocytochemistry/ Immunofluorescence: Myeloperoxidase/MPO Antibody (A1F2-R) [NBP3-32623] - Immunocytochemistry analysis of HL-60 cells labeling Myeloperoxidase/MPO with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32623_mouse-myeloperoxidase-mpo-mab-a1f2-r-29202410484828.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-278202416285219.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-298202414304184.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunocytochemistry analysis of U-87 MG cells labeling 5'-Nucleotidase/CD73 with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-2920241192315.jpg)