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![Immunoprecipitation:HPRT AntibodyNBP3-29542] -Detection of human HPRT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HPRT1 antibody NBP3-29542 (lot NBP3-29542-1) used for IP at 6 µg per reaction. HPRT1 was also immunoprecipitated by rabbit anti-HPRT1.. For blotting immunoprecipitated HPRT1, NBP3-29542 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29542_rabbit-hprt-pab-301220241226255.jpg "Immunoprecipitation:HPRT AntibodyNBP3-29542] -Detection of human HPRT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HPRT1 antibody NBP3-29542 (lot NBP3-29542-1) used for IP at 6 µg per reaction. HPRT1 was also immunoprecipitated by rabbit anti-HPRT1.. For blotting immunoprecipitated HPRT1, NBP3-29542 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: 4EBP1 [p Ser65] Antibody [NBP3-13171] - Wild-type (WT) and 4EBP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Ser65) antibody (NBP3-13171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/4EBP1-[p-Ser65]-Antibody-Western-Blot-NBP3-13171-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 [p Ser65] Antibody [NBP3-13171] - Wild-type (WT) and 4EBP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Ser65) antibody (NBP3-13171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 4EBP1 [p Ser65] Antibody [NBP3-13171] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Ser65) antibody (NBP3-13171) diluted at 1:500.](http://images.novusbio.com/fullsize/4EBP1-[p-Ser65]-Antibody-Western-Blot-NBP3-13171-img0001.jpg)
![Immunoprecipitation:Aconitase 2 AntibodyNBP3-29844] -Detection of human ACO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACO2 antibody NBP3-29844 (lot NBP3-29844-1) used for IP at 6 µg per reaction. ACO2 was also immunoprecipitated by rabbit anti-ACO2.. For blotting immunoprecipitated ACO2, NBP3-29844 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29844_rabbit-aconitase-2-pab-301220241556519.jpg "Immunoprecipitation:Aconitase 2 AntibodyNBP3-29844] -Detection of human ACO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACO2 antibody NBP3-29844 (lot NBP3-29844-1) used for IP at 6 µg per reaction. ACO2 was also immunoprecipitated by rabbit anti-ACO2.. For blotting immunoprecipitated ACO2, NBP3-29844 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![ELISA: Rat Tyrosine Hydroxylase ELISA Kit (Colorimetric) [NBP3-06922] - Samples were spiked with high concentrations of Rat Tyrosine Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06922_rat-tyrosine-hydroxylase-elisa-kit-colorimetric-132202416225266.jpg "ELISA: Rat Tyrosine Hydroxylase ELISA Kit (Colorimetric) [NBP3-06922] - Samples were spiked with high concentrations of Rat Tyrosine Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Tyrosine Hydroxylase ELISA Kit (Colorimetric) [NBP3-06922] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06922_rat-tyrosine-hydroxylase-elisa-kit-colorimetric-4320249133218.jpg)
![ELISA: Mouse Carbonic Anhydrase II/CA2 ELISA Kit (Colorimetric) [NBP2-70055] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase II/CA2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70055_mouse-carbonic-anhydrase-ii-ca2-elisa-kit-colorimetric-1322024168532.jpg "ELISA: Mouse Carbonic Anhydrase II/CA2 ELISA Kit (Colorimetric) [NBP2-70055] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase II/CA2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Carbonic Anhydrase II/CA2 ELISA Kit (Colorimetric) [NBP2-70055] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70055_mouse-carbonic-anhydrase-ii-ca2-elisa-kit-colorimetric-432024911567.jpg)
![ELISA: Human Irisin/FNDC5 ELISA Kit (Colorimetric) [NBP3-08117] - Samples were spiked with high concentrations of Human Irisin/FNDC5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08117_human-irisin-fndc5-elisa-kit-colorimetric-132202416121345.jpg "ELISA: Human Irisin/FNDC5 ELISA Kit (Colorimetric) [NBP3-08117] - Samples were spiked with high concentrations of Human Irisin/FNDC5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Irisin/FNDC5 ELISA Kit (Colorimetric) [NBP3-08117] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08117_human-irisin-fndc5-elisa-kit-colorimetric-4320249501.jpg)