| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-62560-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12868-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59454-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89658-50ul | |||
![Western Blot: Caspase-1 Antibody - (cleaved) [NBP3-13233] - Untreated (-) and treated (+) THP-1 whole cell extracts and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with Caspase-1 (cleaved Asp297) antibody (NBP3-13233) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-cleaved-Western-Blot-NBP3-13233-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Caspase-1 Antibody - (cleaved) [NBP3-13233] - Untreated (-) and treated (+) THP-1 whole cell extracts and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with Caspase-1 (cleaved Asp297) antibody (NBP3-13233) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Caspase-1 Antibody - (cleaved) [NBP3-13233] - Treated (+) THP-1 whole cell extracts and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cleaved Caspase-1 (p20) antibody (NBP3-13233) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-cleaved-Western-Blot-NBP3-13233-img0001.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody (HL2298) - Azide and BSA Free [NBP3-25483] - GLP1R antibody [HL2298] detects GLP1R protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. GLP1R stained by GLP1R antibody [HL2298] (NBP3-25483) diluted at 1:20000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25483_rabbit-glp-1r-mab-hl2298-azide-and-bsa-free-2120249342743.jpg "Immunohistochemistry-Paraffin: GLP-1R Antibody (HL2298) - Azide and BSA Free [NBP3-25483] - GLP1R antibody [HL2298] detects GLP1R protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. GLP1R stained by GLP1R antibody [HL2298] (NBP3-25483) diluted at 1:20000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![GLP1R antibody [HL2298] detects GLP1R protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.GLP1R stained by GLP1R antibody [HL2298] (NBP3-25483) diluted at 1:2000 and competitor's antibody (ab218532) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25483_rabbit-glp-1r-mab-hl2298-azide-and-bsa-free-immunohistochemistry-paraffin-2611202518375017.jpg)
![ELISA: Ferritin Antibody (321B-7 (05)) [NB100-62560] - Analysis of human ferritin using Mouse anti Human ferritin antibody, clone F23 as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 as a detection reagent with recombinant human ferritin as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm. Normal serum (green) and plasma (orange) samples are included undiluted.](http://images.novusbio.com/fullsize/Ferritin-Antibody-321B-7-05-ELISA-NB100-62560-img0001.jpg "ELISA: Ferritin Antibody (321B-7 (05)) [NB100-62560] - Analysis of human ferritin using Mouse anti Human ferritin antibody, clone F23 as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 as a detection reagent with recombinant human ferritin as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm. Normal serum (green) and plasma (orange) samples are included undiluted.")
![ELISA: Ferritin Antibody (321B-7 (05)) [NB100-62560] - Analysis of human ferritin using Mouse anti Human ferritin antibody, clone F31 as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 as a detection reagent with recombinant human ferritin as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm. Normal serum (green) and plasma (orange) samples are included undiluted](http://images.novusbio.com/fullsize/Ferritin-Antibody-321B-7-05-ELISA-NB100-62560-img0002.jpg)
![ELISA: Ferritin Antibody (321B-7 (05)) [NB100-62560] - Analysis of human ferritin using Mouse anti Human ferritin antibody, clone F23 as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 as a detection reagent with purified human ferritin as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm. Normal serum (green) and plasma (orange) samples are included undiluted](http://images.novusbio.com/fullsize/Ferritin-Antibody-321B-7-05-ELISA-NB100-62560-img0003.jpg)
![ELISA: Ferritin Antibody (321B-7 (05)) [NB100-62560] - Analysis of human ferritin using Mouse anti Human ferritin antibody, clone F31 as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 as a detection reagent with purified human ferritin as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm. Normal serum (green) and plasma (orange) samples are included undiluted.](http://images.novusbio.com/fullsize/Ferritin-Antibody-321B-7-05-ELISA-NB100-62560-img0004.jpg)
![Western Blot: PRAS40 Antibody [NBP1-55261] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate](http://images.novusbio.com/fullsize/PRAS40-Antibody-Western-Blot-NBP1-55261-img0004.jpg "Western Blot: PRAS40 Antibody [NBP1-55261] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate")
![Immunohistochemistry: PRAS40 Antibody [NBP1-55261] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic in endothelial cells in blood vessels Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-NBP1-55261-img0003.jpg)
![Western Blot: ARG2 Antibody (1Y5U3) [NBP3-15697] - Western blot analysis of extracts of 293T cells, using Arginase 2 (ARG2) antibody (NBP3-15697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ARG2-Antibody-1Y5U3-Western-Blot-NBP3-15697-img0002.jpg "Western Blot: ARG2 Antibody (1Y5U3) [NBP3-15697] - Western blot analysis of extracts of 293T cells, using Arginase 2 (ARG2) antibody (NBP3-15697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: ARG2 Antibody (1Y5U3) [NBP3-15697] - Immunohistochemistry of paraffin-embedded human prostate cancer using Arginase 2 (ARG2) Rabbit mAb (NBP3-15697) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ARG2-Antibody-1Y5U3-Immunohistochemistry-Paraffin-NBP3-15697-img0001.jpg)
![Western Blot: PGM1 Antibody (AT84G2) [NBP3-12868] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-12868 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: 293T cell lysate. Lane 2: HepG2 cell lysate. Lane 3: NIH/3T3 cell lysate. Lane 4: Jurkat cell lysate. Lane 5: HeLa cell lysate. Lane 6: U87MG cell lysate](http://images.novusbio.com/fullsize/PGM1-Antibody-AT84G2-Western-Blot-NBP3-12868-img0002.jpg "Western Blot: PGM1 Antibody (AT84G2) [NBP3-12868] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-12868 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: 293T cell lysate. Lane 2: HepG2 cell lysate. Lane 3: NIH/3T3 cell lysate. Lane 4: Jurkat cell lysate. Lane 5: HeLa cell lysate. Lane 6: U87MG cell lysate")
![Immunocytochemistry/Immunofluorescence: PGM1 Antibody (AT84G2) [NBP3-12868] - HeLa cells were stained with NBP3-12868 (1:100). Alexa Fluor 488-conjugated secondary antibody was used (green). DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/PGM1-Antibody-AT84G2-Immunocytochemistry-Immunofluorescence-NBP3-12868-img0001.jpg)
![Western Blot: PGM1 Antibody (84G2) [NBP2-59454] - Lane 1: 293T cell lysates, Lane 2: HepG2 cell lysates, Lane 3: NIH/3T3 cell lysates, Lane 4: Jurkat cell lysates, Lane 5: HeLa cell lysates, Lane 6: U87MG cell lysates](http://images.novusbio.com/fullsize/PGM1-Antibody-84G2-Western-Blot-NBP2-59454-img0001.jpg "Western Blot: PGM1 Antibody (84G2) [NBP2-59454] - Lane 1: 293T cell lysates, Lane 2: HepG2 cell lysates, Lane 3: NIH/3T3 cell lysates, Lane 4: Jurkat cell lysates, Lane 5: HeLa cell lysates, Lane 6: U87MG cell lysates")
![Immunocytochemistry/Immunofluorescence: PGM1 Antibody (84G2) [NBP2-59454] - Analysis of PGM1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PGM1-Antibody-84G2-Immunocytochemistry-Immunofluorescence-NBP2-59454-img0002.jpg)
![Western Blot: CD38 Antibody (003) [NBP2-89658] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary antibody goat anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:50 kDa.](http://images.novusbio.com/fullsize/CD38-Antibody-003-Western-Blot-NBP2-89658-img0001.jpg "Western Blot: CD38 Antibody (003) [NBP2-89658] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary antibody goat anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:50 kDa.")
![Immunoprecipitation: CD38 Antibody (003) [NBP2-89658] - Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-CD38 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD38 rabbit monoclonal antibody at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size: 45 kDa.](http://images.novusbio.com/fullsize/CD38-Antibody-003-Immunoprecipitation-NBP2-89658-img0002.jpg)
![Western Blot: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Western blot analysis of LAMP-2/CD107b on different lysates with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32523) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32523_mouse-lamp-2-cd107b-mab-d3-d8-d3-r-278202416331251.jpg "Western Blot: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Western blot analysis of LAMP-2/CD107b on different lysates with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32523) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32523) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32523_mouse-lamp-2-cd107b-mab-d3-d8-d3-r-298202414304116.jpg)
![Immunohistochemistry-Paraffin: PDE8A Antibody - BSA Free [NB110-40457] - Analysis of anti-PDE8A antibody with human prostate at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PDE8A-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB110-40457-img0001.jpg "Immunohistochemistry-Paraffin: PDE8A Antibody - BSA Free [NB110-40457] - Analysis of anti-PDE8A antibody with human prostate at concentration 10 ug/ml.")
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0002.jpg "Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).")
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0001.jpg)