| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77689SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89736-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25266 | |||
| Azide and BSA Free | NBP2-25266SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93781-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87053-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21996-50ul | |||
![Immunocytochemistry/Immunofluorescence: ABCB5 Antibody [NBP1-77689] - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/ABCB5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77689-img0003.jpg "Immunocytochemistry/Immunofluorescence: ABCB5 Antibody [NBP1-77689] - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Western-Blot-NBP2-89736-img0002.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Analysis of Human AGT expression on HepG2 cells. The cells were treated ,stained with purified anti-Human AGT, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Flow-Cytometry-NBP2-89736-img0004.jpg)
![Immunoprecipitation: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SerpinA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SerpinA8 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Immunoprecipitation-NBP2-89736-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Staining of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89736 antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89736_rabbit-monoclonal-serpin-a8-angiotensinogen-antibody-001-23520251129370.png)
![Western Blot: Noxa Antibody (69N15C9) [NBP2-25251] - Western blot analysis of mouse A) Noxa recombinant protein, B) thymus and C) ovary using Noxa antibody at 1 ug/mL, 5 ug/mL and 5 ug/mL, respectively.](http://images.novusbio.com/fullsize/Noxa-Antibody-69N15C9-Western-Blot-NBP2-25251-img0001.jpg "Western Blot: Noxa Antibody (69N15C9) [NBP2-25251] - Western blot analysis of mouse A) Noxa recombinant protein, B) thymus and C) ovary using Noxa antibody at 1 ug/mL, 5 ug/mL and 5 ug/mL, respectively.")
![Western Blot: IPMK Antibody (HL2244) - Azide and BSA Free [NBP3-25536] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [HL2244] (NBP3-25536) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25536_rabbit-ipmk-mab-hl2244-azide-and-bsa-free-2120249432844.jpg "Western Blot: IPMK Antibody (HL2244) - Azide and BSA Free [NBP3-25536] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [HL2244] (NBP3-25536) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: IPMK Antibody (HL2244) - Azide and BSA Free [NBP3-25536] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [HL2244] (NBP3-25536) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25536_rabbit-ipmk-mab-hl2244-azide-and-bsa-free-2120249351384.jpg)
![Western Blot: IPMK Antibody (HL2244) - Azide and BSA Free [NBP3-25536] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [HL2244] (NBP3-25536) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25536_rabbit-ipmk-mab-hl2244-azide-and-bsa-free-2120249355582.jpg)
![Western Blot: IPMK Antibody (HL2244) - Azide and BSA Free [NBP3-25536] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [HL2244] (NBP3-25536) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25536_rabbit-ipmk-mab-hl2244-azide-and-bsa-free-2120249432883.jpg)
![Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ABCB5-Antibody-Western-Blot-NB100-93365-img0002.jpg "Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: ABCB5 Antibody [NB100-93365] - analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ABCB5-Antibody-Flow-Cytometry-NB100-93365-img0001.jpg)
![Western Blot: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781] - Western blot analysis of various lysates, using Pyruvate Carboxylase Rabbit pAb (A6301) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp2-93781_rabbit-polyclonal-pyruvate-carboxylase-antibody-5220251931355.jpg "Western Blot: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781] - Western blot analysis of various lysates, using Pyruvate Carboxylase Rabbit pAb (A6301) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.")
![Immunoprecipitation: Pyruvate Carboxylase Antibody [NBP2-93781] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Pyruvate Carboxylase antibody (NBP2-93781). Western blot was performed from the immunoprecipitate using Pyruvate Carboxylase antibody (NBP2-93781) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunoprecipitation-NBP2-93781-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-93781] - Analysis of U2OS cells using Pyruvate Carboxylase . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93781-img0001.jpg)
![Western Blot: IPMK Antibody [H00253430-B01P] - Analysis of IPMK expression in transfected 293T cell line by IPMK polyclonal antibody. Lane1:IPMK transfected lysate(45.76 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/fullsize/IPMK-Antibody-Western-Blot-H00253430-B01P-img0004.jpg "Western Blot: IPMK Antibody [H00253430-B01P] - Analysis of IPMK expression in transfected 293T cell line by IPMK polyclonal antibody. Lane1:IPMK transfected lysate(45.76 KDa). Lane2:Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: IPMK Antibody [H00253430-B01P] - Analysis of purified antibody to IPMK on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/IPMK-Antibody-Immunocytochemistry-Immunofluorescence-H00253430-B01P-img0003.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-87053] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-87053-img0006.jpg "Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-87053] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.")
![Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis using Anti-PC antibody NBP2-55361 (A) shows similar pattern to independent antibody NBP2-33407 (B).](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP2-55361-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis using Anti-PC antibody NBP2-55361 (A) shows similar pattern to independent antibody NBP2-33407 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-55361] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55361-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis in human cell lines HeLa and U-251M. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP2-55361-img0003.jpg)
![Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-278202416331212.jpg "Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32721) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-2982024142096.jpg)
![Western Blot: Hsp70 interacting protein HIP Antibody (4A3G8) [NBP3-16829] - Western blot analysis of extracts of various cell lines, using Hsp70 interacting protein HIP Rabbit mAb (NBP3-16829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Hsp70-interacting-protein-HIP-Antibody-4A3G8-Western-Blot-NBP3-16829-img0001.jpg "Western Blot: Hsp70 interacting protein HIP Antibody (4A3G8) [NBP3-16829] - Western blot analysis of extracts of various cell lines, using Hsp70 interacting protein HIP Rabbit mAb (NBP3-16829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Hsp70 interacting protein HIP Antibody (4A3G8) [NBP3-16829] - Western blot analysis of various lysates, using Hsp70 interacting protein HIP Rabbit mAb at 1 : 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16829_rabbit-hsp70-interacting-protein-hip-mab-4a3g8-8220252010472.jpg)
![Western Blot: Hsp70 interacting protein HIP Antibody (4A3G8) [NBP3-16829] - Western blot analysis of extracts of various cell lines, using Hsp70 interacting protein HIP Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16829_rabbit-hsp70-interacting-protein-hip-mab-4a3g8-822025208481.jpg)
![Western Blot: Hsp70 interacting protein HIP Antibody (SR1817) [NBP3-21996] - Western blot analysis using NBP3-21996 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21996_rabbit-hsp70-interacting-protein-hip-mab-sr1817-57202316582178.jpg "Western Blot: Hsp70 interacting protein HIP Antibody (SR1817) [NBP3-21996] - Western blot analysis using NBP3-21996 in 293T cell lysate.")