| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90022-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97227-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90461-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97088-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89432-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90352-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89345-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90076-50ul | |||
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Western-Blot-NBP2-90022-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate0.5 uL anti-TPH1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TPH1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 60 kDa](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Immunoprecipitation-NBP2-90022-img0001.jpg)
![Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-97227] - Immunochemical staining of human NMNAT-1 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NMNAT-1-Antibody-Immunohistochemistry-Paraffin-NBP2-97227-img0002.jpg "Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-97227] - Immunochemical staining of human NMNAT-1 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: NMNAT-1 Antibody [NBP2-97227] - Immunochemical staining of human NMNAT-1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NMNAT-1-Antibody-Immunohistochemistry-Paraffin-NBP2-97227-img0001.jpg)
![Immunohistochemistry: Monoglyceride Lipase Antibody [NBP3-30968] - Immunohistochemical staining of human adipose tissue using MGL antibody (Cat. No. NBP3-30968). Antibody used at 2 ug/ml. 40x magnification.](http://images.novusbio.com/fullsize/nbp3-30968_rabbit-monoglyceride-lipase-pab-811202420592166.jpg "Immunohistochemistry: Monoglyceride Lipase Antibody [NBP3-30968] - Immunohistochemical staining of human adipose tissue using MGL antibody (Cat. No. NBP3-30968). Antibody used at 2 ug/ml. 40x magnification.")
![Flow Cytometry: RBP4/Retinol-Binding Protein 4 Antibody (008) [NBP2-90461] - Flow cytometric analysis of Rat CD59 expression on SD rat bone marrow cells. Cells were stained with purified anti-Rat CD59, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-008-Flow-Cytometry-NBP2-90461-img0001.jpg "Flow Cytometry: RBP4/Retinol-Binding Protein 4 Antibody (008) [NBP2-90461] - Flow cytometric analysis of Rat CD59 expression on SD rat bone marrow cells. Cells were stained with purified anti-Rat CD59, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/ Immunofluorescence: RBP4/Retinol-Binding Protein 4 Antibody (008) [NBP2-90461] - Immunofluorescence staining of RBP4/Retinol-Binding Protein 4 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90461 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/nbp2-90461_rabbit-monoclonal-rbp4-retinol-binding-protein-4-antibody-008-235202512205528.png)
![Immunohistochemistry-Paraffin: Gastric Lipase Antibody [NBP2-97088] - Immunochemical staining of human Gastric Lipase in human stomach with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Gastric-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP2-97088-img0001.jpg "Immunohistochemistry-Paraffin: Gastric Lipase Antibody [NBP2-97088] - Immunochemical staining of human Gastric Lipase in human stomach with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Gastric Lipase Antibody [NBP2-97088] - Immunochemical staining of human Gastric Lipase in human stomach with NBP2-97088 (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-97088_rabbit-polyclonal-gastric-lipase-antibody-235202514331555.png)
![Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432] - Staining of CD62E in TNF-a stimulated HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89432 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/nbp2-89432_rabbit-monoclonal-e-selectin-cd62e-antibody-208-235202514371334.png "Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432] - Staining of CD62E in TNF-a stimulated HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89432 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (012) [NBP2-90352] - Staining of mouse CA12 in mouse intestine with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90352-img0002.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (012) [NBP2-90352] - Staining of mouse CA12 in mouse intestine with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (012) [NBP2-90352] - Staining of mouse CA12 in mouse kidney with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90352-img0001.jpg)
![Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-002-Flow-Cytometry-NBP2-89345-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (002) [NBP2-89345] - Staining of LEP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89345 (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm .](http://images.novusbio.com/fullsize/nbp2-89345_rabbit-monoclonal-leptin-ob-antibody-002-23520251129371.png)
![Immunohistochemistry: alcohol dehydrogenase 4 Antibody [NBP3-10572] - Immunohistochemical analysis of fetal liver cell lysate tissue at an antibody concentration of 1.25 ug/ml.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-4-Antibody-Immunohistochemistry-NBP3-10572-img0001.jpg "Immunohistochemistry: alcohol dehydrogenase 4 Antibody [NBP3-10572] - Immunohistochemical analysis of fetal liver cell lysate tissue at an antibody concentration of 1.25 ug/ml.")
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE55-54) [NBP3-33134] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33134) at 1/1,000 dilution. Lane 1: SH-SY5Y whole cell lysate Lane 2: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes whole cell lysate Lane 3: SH-SY5Y treated with pp for 1 hour whole cell lysate Lane 4: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 277 kDa Observed band size: 277 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33134) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33134_rabbit-acetyl-coa-carboxylase-alpha-acaca-p-ser79-mab-28820241423312.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE55-54) [NBP3-33134] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33134) at 1/1,000 dilution. Lane 1: SH-SY5Y whole cell lysate Lane 2: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes whole cell lysate Lane 3: SH-SY5Y treated with pp for 1 hour whole cell lysate Lane 4: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 277 kDa Observed band size: 277 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33134) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: CD98 Antibody (071) [NBP2-90076] - Staining of human SLC3A2 in human placenta with rabbit monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/CD98-Antibody-071-Immunohistochemistry-Paraffin-NBP2-90076-img0002.jpg "Immunohistochemistry-Paraffin: CD98 Antibody (071) [NBP2-90076] - Staining of human SLC3A2 in human placenta with rabbit monoclonal antibody (1:500).")
![Immunohistochemistry-Paraffin: CD98 Antibody (071) [NBP2-90076] - Staining of human SLC3A2 in human kidney with rabbit monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/CD98-Antibody-071-Immunohistochemistry-Paraffin-NBP2-90076-img0001.jpg)