| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35729-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05061-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35805-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92604-0.02ml | |||
![Western Blot: EDEM2 Antibody [NBP3-35729] - Western blot analysis of various lysates using EDEM2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35729_rabbit-edem2-pab-912202415133474.jpg "Western Blot: EDEM2 Antibody [NBP3-35729] - Western blot analysis of various lysates using EDEM2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: EDEM2 Antibody [NBP3-35729] - Immunofluorescence analysis of L929 cells using EDEM2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35729_rabbit-edem2-pab-912202414163060.jpg)
![Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MID1-Antibody-Western-Blot-NBP3-05061-img0003.jpg "Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MID1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05061-img0001.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-5D2H1-Western-Blot-NBP3-16541-img0002.jpg "Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Immunofluorescence analysis of HeLa cells using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-5D2H1-Immunocytochemistry-Immunofluorescence-NBP3-16541-img0001.jpg)
![Western Blot: ABCE1 Antibody (1N4I7) [NBP3-16763] - Western blot analysis of extracts of various cell lines, using ABCE1 Rabbit mAb (NBP3-16763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/ABCE1-Antibody-1N4I7-Western-Blot-NBP3-16763-img0002.jpg "Western Blot: ABCE1 Antibody (1N4I7) [NBP3-16763] - Western blot analysis of extracts of various cell lines, using ABCE1 Rabbit mAb (NBP3-16763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody (1N4I7) [NBP3-16763] - Immunofluorescence analysis of NIH-3T3 cells using ABCE1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16763_rabbit-abce1-mab-1n4i7-82202520843.jpg)
![Western Blot: iNOS Antibody (3L0U6) [NBP3-16273] - Western blot analysis of lysates from RAW 264.7 cells using iNOS Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Raw264.7 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16273_rabbit-inos-mab-3l0u6-5220251265374.jpg "Western Blot: iNOS Antibody (3L0U6) [NBP3-16273] - Western blot analysis of lysates from RAW 264.7 cells using iNOS Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Raw264.7 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273] - Confocal imaging of RAW 264.7 cells (treated with LPS) and RAW 264.7 cells(untreated) using iNOS Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16273_rabbit-inos-mab-3l0u6-52202511392210.jpg)
![Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202414213796.jpg "Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: TBC1D4 Antibody [NBP3-35805] - Western blot analysis of lysates from Rat skeletal muscle, using TBC1D4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202415133469.jpg)
![Western Blot: FHIT Antibody [NBP3-27889] - Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 u/ml.](http://images.novusbio.com/fullsize/nbp3-27889_rabbit-fhit-pab-2711202420414526.gif "Western Blot: FHIT Antibody [NBP3-27889] - Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 u/ml.")
![Immunocytochemistry/ Immunofluorescence: FHIT Antibody [NBP3-27889] - Immunofluorescence of FHIT in HeLa cells with FHIT antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp3-27889_rabbit-fhit-pab-2711202419201569.jpg)
![Western Blot: ABCD1 Antibody [NBP2-92604] - Analysis of extracts of various cell lines, using ABCD1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/ABCD1-Antibody-Western-Blot-NBP2-92604-img0002.jpg "Western Blot: ABCD1 Antibody [NBP2-92604] - Analysis of extracts of various cell lines, using ABCD1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: ABCD1 Antibody - Azide and BSA Free [NBP2-92604] - Immunofluorescence analysis of NIH/3T3 cells using ABCD1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92604_rabbit-polyclonal-abcd1-antibody-42202514264440.jpg%3Ft=1709092081)
![Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Western blot of human adrenal medulla. The antibody is specific for the ~75 kDa DBH protein.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NB300-112-img0002.jpg "Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Western blot of human adrenal medulla. The antibody is specific for the ~75 kDa DBH protein.")
![Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Human adrenal medulla lysate showing specific immunolabeling of the ~75k DBH protein.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NB300-112-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (GL2A7) [NBP1-97499] - Mouse astrocytes stained with Anti-LAMP-2/CD107b. Cells were fixed using paraformaldehyde-lysine-periodate (including 4% paraformaldehyde) and permeabilized using 0.01% saponin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-GL2A7-Immunocytochemistry-Immunofluorescence-NBP1-97499-img0008.jpg "Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (GL2A7) [NBP1-97499] - Mouse astrocytes stained with Anti-LAMP-2/CD107b. Cells were fixed using paraformaldehyde-lysine-periodate (including 4% paraformaldehyde) and permeabilized using 0.01% saponin.")
![Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32818) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32818_rabbit-pi-3-kinase-p85-alpha-p-tyr199-p-tyr467-mab-288202412442996.jpg "Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32818) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 1mM Sodium orthovanadate for 30 minutes labeling PI 3-Kinase p85 alpha with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32818_rabbit-pi-3-kinase-p85-alpha-p-tyr199-p-tyr467-mab-29202410334087.jpg)
![Immunohistochemistry: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31995) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31995_mouse-acsl5-mab-a9d4-r-29820241492626.jpg "Immunohistochemistry: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31995) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31995) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31995_mouse-acsl5-mab-a9d4-r-3920248453515.jpg)