| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90437-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98267-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06252-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90108-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99999-50ul | |||
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (004) [NBP2-90437] - Immunochemical staining of mouse SR-AI/MSR in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-004-Immunohistochemistry-Paraffin-NBP2-90437-img0003.jpg "Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (004) [NBP2-90437] - Immunochemical staining of mouse SR-AI/MSR in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).")
![Flow Cytometry: SR-AI/MSR Antibody (004) [NBP2-90437] - Flow cytometric analysis of Mouse SR-AI/MSR expression on Raw264.7 cells. Cells were stained with purified anti-Mouse SR-AI/MSR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-004-Flow-Cytometry-NBP2-90437-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody (004) [NBP2-90437] - Confocal immunofluorescence analysis of Mouse SR-AI/MSR1 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90437 antibody (1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/nbp2-90437_rabbit-monoclonal-sr-ai-msr-antibody-004-235202511444470.png)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Western-Blot-NBP2-98350-img0007.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Staining of mouse Carbonic Anhydrase VIII/CA8 in mouse brain with rabbit polyclonal antibody at 1:25000 dilution.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Immunohistochemistry-Paraffin-NBP2-98350-img0003.jpg)
![Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Mouse Carbonic Anhydrase VIII/CA8 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 0.5 ul anti-Mouse Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Immunoprecipitation-NBP2-98350-img0005.jpg)
![Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Lane A: Mouse liver tissue lysateLane B: Mouse skeletal muscle tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Western-Blot-NBP2-98267-img0005.jpg "Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Lane A: Mouse liver tissue lysateLane B: Mouse skeletal muscle tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa")
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse skeletal muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0004.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse heart with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0002.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0003.jpg)
![Western Blot: LYPLAL1 Antibody [NBP3-06252] - Anti-LYPLAL1 rabbit polyclonal antibody at 1:500 dilutionLane A: COCLO205 Whole Cell LysateLane B: RT-4 Whole Cell LysateLane C: U-251MG Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa](http://images.novusbio.com/fullsize/LYPLAL1-Antibody-Western-Blot-NBP3-06252-img0001.jpg "Western Blot: LYPLAL1 Antibody [NBP3-06252] - Anti-LYPLAL1 rabbit polyclonal antibody at 1:500 dilutionLane A: COCLO205 Whole Cell LysateLane B: RT-4 Whole Cell LysateLane C: U-251MG Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa")
![Immunohistochemistry-Paraffin: LYPLAL1 Antibody [NBP3-06252] - Immunochemical staining of human LYPLAL1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/LYPLAL1-Antibody-Immunohistochemistry-Paraffin-NBP3-06252-img0002.jpg)
![Immunoprecipitation: LYPLAL1 Antibody [NBP3-06252] - LYPLAL1 was immunoprecipitated using:Lane A:0.5 mg U-251MG Whole Cell Lysate4 uL anti-LYPLAL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-LYPLAL1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :29 kDa](http://images.novusbio.com/fullsize/LYPLAL1-Antibody-Immunoprecipitation-NBP3-06252-img0003.jpg)
![Immunohistochemistry-Paraffin: PHPT1 Antibody (001) [NBP2-90108] - Immunochemical staining of human PHPT1 in human skeletal muscle with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PHPT1-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90108-img0001.jpg "Immunohistochemistry-Paraffin: PHPT1 Antibody (001) [NBP2-90108] - Immunochemical staining of human PHPT1 in human skeletal muscle with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections).")
![<b>Biological Strategies Validation. </b>Flow Cytometry: PHPT1 Antibody (001) [NBP2-90108] - Flow cytometric analysis of Human PHPT1 expression on THP1 cells . The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PHPT1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/PHPT1-Antibody-001-Flow-Cytometry-NBP2-90108-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PHPT1 Antibody (001) [NBP2-90108] - Immunofluorescence staining of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90108 antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90108_rabbit-monoclonal-phpt1-antibody-001-23520251144445.png)
![Western Blot: PHPT1 Antibody [NBP2-99999] - Lane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa](http://images.novusbio.com/fullsize/PHPT1-Antibody-Western-Blot-NBP2-99999-img0005.jpg "Western Blot: PHPT1 Antibody [NBP2-99999] - Lane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa")
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-99999] - Staining of human PHPT1 in human skeletal muscle with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP2-99999-img0003.jpg)
![Immunoprecipitation: PHPT1 Antibody [NBP2-99999] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg THP-1 Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PHPT1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PHPT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunoprecipitation-NBP2-99999-img0004.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Western-Blot-NBP2-67191-img0002.jpg "Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0001.jpg)
![Western Blot: MafB Antibody [NBP2-87767] - Host: Rabbit. Target Name: MAFB. Sample Type: Lane 1: Non-overexpressed vector control lysate. Lane 2: Transient overexpression lysate of MAFB. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/MafB-Antibody-Western-Blot-NBP2-87767-img0003.jpg "Western Blot: MafB Antibody [NBP2-87767] - Host: Rabbit. Target Name: MAFB. Sample Type: Lane 1: Non-overexpressed vector control lysate. Lane 2: Transient overexpression lysate of MAFB. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: MafB Antibody [NBP2-87767] - Human kidney](http://images.novusbio.com/fullsize/MafB-Antibody-Immunohistochemistry-NBP2-87767-img0001.jpg)
![Western Blot: MafB Antibody [NBP2-87767] - Host: Rabbit. Target: MAFB. Positive control (+): Mouse spleen (M-SP). Negative control (-): Mouse intestine (M-IN). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/MafB-Antibody-Western-Blot-NBP2-87767-img0002.jpg)
![Western Blot: MafB Antibody [NBP2-87767] - Host: Rabbit. Target Name: MAFB. Sample Type: Human Lung. Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/MafB-Antibody-Western-Blot-NBP2-87767-img0004.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-278202416331274.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32111) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-298202414333644.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunofluorescence analysis of paraffin-embedded mouse kidney tissue labeling Calcium-sensing R/CaSR with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32111, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-29820241492610.jpg)
![Western Blot: Myeloperoxidase/MPO Antibody (PD00-20) [NBP3-32624] - Western blot analysis of Myeloperoxidase/MPO on different lysates with Rabbit anti-Myeloperoxidase/MPO antibody (NBP3-32624) at 1/5,000 dilution. Lane 1: HeLa cell lysate (negative) Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84/55 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32624) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32624_rabbit-myeloperoxidase-mpo-mab-pd00-20-2882024142265.jpg "Western Blot: Myeloperoxidase/MPO Antibody (PD00-20) [NBP3-32624] - Western blot analysis of Myeloperoxidase/MPO on different lysates with Rabbit anti-Myeloperoxidase/MPO antibody (NBP3-32624) at 1/5,000 dilution. Lane 1: HeLa cell lysate (negative) Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84/55 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32624) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Myeloperoxidase/MPO Antibody (PD00-20) [NBP3-32624] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Myeloperoxidase/MPO antibody (NBP3-32624) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32624) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32624_rabbit-myeloperoxidase-mpo-mab-pd00-20-298202414161865.jpg)
![Immunocytochemistry/ Immunofluorescence: Myeloperoxidase/MPO Antibody (PD00-20) [NBP3-32624] - Immunocytochemistry analysis of Jurkat cells labeling Myeloperoxidase/MPO with Rabbit anti-Myeloperoxidase/MPO/MPO antibody (NBP3-32624) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myeloperoxidase/MPO antibody (NBP3-32624) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32624_rabbit-myeloperoxidase-mpo-mab-pd00-20-29202410234294.jpg)
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0015.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.")
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0014.jpg)