| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97749-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00319-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05935-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97693-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-42368 | |||
![Western Blot: HSDL2 Antibody [NBP2-97749] - Anti-HSDL2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/HSDL2-Antibody-Western-Blot-NBP2-97749-img0005.jpg "Western Blot: HSDL2 Antibody [NBP2-97749] - Anti-HSDL2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa")
![Immunohistochemistry-Paraffin: HSDL2 Antibody [NBP2-97749] - Immunochemical staining of human HSDL2 in human liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HSDL2-Antibody-Immunohistochemistry-Paraffin-NBP2-97749-img0004.jpg)
![Immunohistochemistry-Paraffin: HSDL2 Antibody [NBP2-97749] - Immunochemical staining of human HSDL2 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HSDL2-Antibody-Immunohistochemistry-Paraffin-NBP2-97749-img0003.jpg)
![Immunoprecipitation: HSDL2 Antibody [NBP2-97749] - HSDL2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-HSDL2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-HSDL2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/HSDL2-Antibody-Immunoprecipitation-NBP2-97749-img0006.jpg)
![Immunohistochemistry-Paraffin: HSDL2 Antibody - BSA Free [NBP2-97749] - Immunochemical staining of human HSDL2 in human kidney with NBP2-97749 at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-97749_rabbit-polyclonal-hsdl2-antibody-235202521451776.png)
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human liver with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-2352025162812.png "Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human liver with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human epididymis with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-235202511444411.png)
![Immunoprecipitation: DCXR Antibody [NBP3-00319] - DCXR was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate 2 uL NBP3-00319 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00319, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-235202511354130.png)
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human kidney with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-23520251633500.png)
![Western Blot: DCXR Antibody [NBP3-00319] - NBP3-00319 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:27 kDa Observed band size:29 kDa](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-23520251664778.png)
![Western Blot: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:88 kDaObserved band size:83 kDa](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Western-Blot-NBP3-05935-img0001.jpg "Western Blot: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:88 kDaObserved band size:83 kDa")
![Immunohistochemistry-Paraffin: Phospholipase C delta 1 Antibody [NBP3-05935] - Staining of human PLCD1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunohistochemistry-Paraffin-NBP3-05935-img0002.jpg)
![Immunoprecipitation: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A:0.5 mg NIH3T3 Whole Cell Lysate4 uL anti-PLCD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PLCD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 86 kDaObserved band size :90 kDa](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunoprecipitation-NBP3-05935-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Phospholipase C delta 1 Antibody [NBP3-05935] - Staining of PLCD1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-05935 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-05935_rabbit-polyclonal-phospholipase-c-delta-1-antibody-235202511494035.png)
![Immunohistochemistry-Paraffin: PDXDC1 Antibody [NBP2-97693] - Immunochemical staining of human PDXDC1 in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PDXDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-97693-img0004.jpg "Immunohistochemistry-Paraffin: PDXDC1 Antibody [NBP2-97693] - Immunochemical staining of human PDXDC1 in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: PDXDC1 Antibody [NBP2-97693] - Anti-PDXDC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 87 kDa. Observed band size: 94 kDa](http://images.novusbio.com/fullsize/PDXDC1-Antibody-Western-Blot-NBP2-97693-img0003.jpg)
![Immunoprecipitation: PDXDC1 Antibody [NBP2-97693] - PDXDC1 was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 ul anti-PDXDC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PDXDC1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 87 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/PDXDC1-Antibody-Immunoprecipitation-NBP2-97693-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PDXDC1 Antibody [NBP2-97693] - Immunofluorescence staining of PDXDC1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97693 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Golgi apparatus.](http://images.novusbio.com/fullsize/nbp2-97693_rabbit-polyclonal-pdxdc1-antibody-235202516335028.png)
![Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/NBP3-20228_Immunohistochemistry-Paraffin-PLC-delta-3-Antibody-NBP3-20228-Negative-Control-showing-staining-of-paraffin-embedded-Human-Testis-with-no-primary-antibody--5420231130455.jpg "Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.")
![Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NBP3-20228_Immunohistochemistry-Paraffin-PLC-delta-3-Antibody-NBP3-20228-6ug-ml-staining-of-paraffin-embedded-Human-Testis-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--5420231130458.jpg)
![Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/NBP3-20228_Immunohistochemistry-Paraffin-PLC-delta-3-Antibody-NBP3-20228-Negative-Control-showing-staining-of-paraffin-embedded-Human-Kidney-with-no-primary-antibody--5420231130453.jpg)
![Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NBP3-20228_Immunohistochemistry-Paraffin-PLC-delta-3-Antibody-NBP3-20228-6ug-ml-staining-of-paraffin-embedded-Human-Kidney-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--5420231130452.jpg)
![Western Blot: PLC-delta 3 Antibody [NBP3-20228] - (2ug/ml) staining of A549 (A) and Caco-2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NBP3-20228_Western-Blot-PLC-delta-3-Antibody-NBP3-20228-2ug-ml-staining-of-A549-A-and-Caco-2-B-cell-lysate-35ug-protein-in-RIPA-buffer-Detected-by-chemiluminescence--5420231130450.jpg)
![ELISA: Human PGC1 alpha - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-41058] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-41058_human-pgc1-alpha-ready-to-use-elisa-kit-colorimetric-elisa-11112024141143..png "ELISA: Human PGC1 alpha - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-41058] - Standard Curve Reference")
![Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29820241492667.jpg "Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (JE40-00) [NBP3-32195] - Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202410334080.jpg)
![Flow Cytometry: CLPP Antibody (JE40-00) [NBP3-32195] - Flow cytometric analysis of HeLa cells labeling CLPP.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32195, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202416372131.jpg)
![Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-298202414262694.jpg "Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Granulin Antibody (JE37-73) [NBP3-32371] - Immunocytochemistry analysis of THP-1 cells labeling Granulin with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution.<br/><br/> Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-2920241114832.jpg)
![Flow Cytometry: Granulin Antibody (JE37-73) [NBP3-32371] - Flow cytometric analysis of THP-1 cells labeling Granulin.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32371, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-29202416453258.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Western-Blot-NBP2-67164-img0005.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate")
![Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of paraffin-embedded human kidney tissue using anti-SPHK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Immunohistochemistry-Paraffin-NBP2-67164-img0002.jpg)
![Flow Cytometry: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of Raji cells with SPHK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Flow-Cytometry-NBP2-67164-img0001.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Western-Blot-NBP2-67164-img0004.jpg)
![Western blot analysis on different lysates with NBP2-67164 at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: Mouse lung tissue lysate (30 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane) Predicted band size: 43 kDa Observed band size: 43-51 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67164 at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67164_-western-blot-639204586673566486.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution.<br/><br/> Lane 1: U-87 MG cell lysate<br/> Lane 2: U-87 MG cell lysate (no heat)<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 63 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 24 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-278202416285219.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution.<br/><br/> Lane 1: U-87 MG cell lysate<br/> Lane 2: U-87 MG cell lysate (no heat)<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 63 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 24 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-298202414304184.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunocytochemistry analysis of U-87 MG cells labeling 5'-Nucleotidase/CD73 with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-2920241192315.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate<br/> Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate<br/> Lane 3: HepG2 cell lysate<br/> Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 93 kDa<br/> Observed band size: 120 kDa<br/><br/> Exposure time: 1minute 2 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate<br/> Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate<br/> Lane 3: HepG2 cell lysate<br/> Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 93 kDa<br/> Observed band size: 120 kDa<br/><br/> Exposure time: 1minute 2 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western blot analysis on different lysates with NBP3-32424 at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate (With sonication) (15 µg/Lane) Lane 3: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate (Without sonication) (15 µg/Lane) Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32424 at 1/5,000 dilution was used at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_-western-blot-639204597147563479.jpg)
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32105) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29820241420924.jpg "Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32105) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunocytochemistry analysis of THP-1 cells labeling Carbonic Anhydrase II/CA2 with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution.<br/><br/> Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29202410334053.jpg)
![Western blot analysis on different lysates with NBP3-32105 at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32105 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32105_-western-blot-639204590077585032.jpg)