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![Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735] - Western blot analysis of extracts of MCF7 cells, using EBI3 Rabbit mAb (NBP3-15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/EBI3-Antibody-9J1H4-Western-Blot-NBP3-15735-img0004.jpg "Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735] - Western blot analysis of extracts of MCF7 cells, using EBI3 Rabbit mAb (NBP3-15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735] - Western blot analysis of extracts of various cell lines, using EBI3 Rabbit mAb (NBP3-15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/EBI3-Antibody-9J1H4-Western-Blot-NBP3-15735-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: EBI3 Antibody (9J1H4) [EBI3] - Immunofluorescence analysis of paraffin-embedded rat spleen using EBI3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15735_rabbit-ebi3-mab-9j1h4-5220251733557.jpg)
![Immunocytochemistry/ Immunofluorescence: EBI3 Antibody (9J1H4) [EBI3] - Immunofluorescence analysis of paraffin-embedded human spleen using EBI3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15735_rabbit-ebi3-mab-9j1h4-52202517361216.jpg)
![Immunocytochemistry/ Immunofluorescence: EBI3 Antibody (9J1H4) [EBI3] - Immunofluorescence analysis of paraffin-embedded mouse spleen using EBI3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15735_rabbit-ebi3-mab-9j1h4-522025173357.jpg)
![Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody [NBP3-38178] - Immunofluorescence analysis of U2OS cells using [KO Validated] Cdk5 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-101220241243970.jpg "Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody [NBP3-38178] - Immunofluorescence analysis of U2OS cells using [KO Validated] Cdk5 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: Cdk5 Antibody [NBP3-38178] - Western blot analysis of various lysates using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-1012202411572659.jpg)
![Western Blot: Cdk5 Antibody [NBP3-38178] - Western blot analysis of various lysates using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-101220241155289.jpg)
![Immunoprecipitation: Cdk5 Antibody [NBP3-38178] - Immunoprecipitation analysis of 900 ug extracts of A-549 cells using 3 ug Cdk5 antibody. Western blot was performed from the immunoprecipitate using Cdk5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-1012202411552850.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody [NBP3-38178] - Western Blot analysis of lysates from wild type (WT) and Cdk5 knockout (KO) 293T cells, using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-31120251744477.jpg)
![Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP3-09107-img0001.jpg "Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control")
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0002.jpg)
![Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Western-Blot-NBP1-44924-img0001.jpg "Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6")
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0003.jpg)
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G6 transfectants with anti-HLA-G (5A6G7) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0002.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0004.jpg "Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line")
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fig. 1. Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody Green - anti-CD95 (LT95) A. JURKAT human peripheral blood T cell leukemia cell line B. RAMOS human Burkitt lymphoma cell line C. CEM human leukemia cell line D. MOLT-4 human acute lymphoblastic T cell leukemia cell line.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0001.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - A surface stain was performed on HeLa with Fas/TNFRSF6/CD95 Antibody (LT95) NB500-503AF647 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0003.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Using the Allophycocyanin direct conjugate Surface staining of human peripheral blood cells with anti-CD95 (LT95) APC.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0002.jpg)
![ELISA: IL-10 Antibody (JES3-12G8) [NBP2-27577] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (Clone JES3-12G8) followed by Streptavidin-HRP.](http://images.novusbio.com/fullsize/IL-10-Antibody-JES3-12G8-ELISA-NBP2-27577-img0001.jpg "ELISA: IL-10 Antibody (JES3-12G8) [NBP2-27577] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (Clone JES3-12G8) followed by Streptavidin-HRP.")
![Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47759-img0002.jpg "Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).")
![Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Human Stomach tissue stained with CCR5 Antibody (12D1).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47759-img0001.jpg)
![Flow Cytometry: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Flow Cytometric Analysis of MCF-7 cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47759-img0003.jpg)
![Western Blot: CCR3 Antibody - Azide Free [NB100-56545] - Analysis of 10 ug of total cell lysate from Daudi cells with this antibody at 2 ug/ml dilution.](http://images.novusbio.com/fullsize/CCR3-Antibody-[Azide-Free]-Western-Blot-NB100-56545-img0002.jpg "Western Blot: CCR3 Antibody - Azide Free [NB100-56545] - Analysis of 10 ug of total cell lysate from Daudi cells with this antibody at 2 ug/ml dilution.")
![ELISA: Human C4 binding protein A ELISA Kit (Colorimetric) [NBP2-60550] - Standard curve.](http://images.novusbio.com/fullsize/Human-C4-binding-protein-A-ELISA-Kit-Colorimetric-ELISA-NBP2-60550-img0001.jpg "ELISA: Human C4 binding protein A ELISA Kit (Colorimetric) [NBP2-60550] - Standard curve.")
![Western Blot: EBI3 Antibody (5P6G8) [NBP2-03942] - Analysis using EBI3 antibody. Mouse EBI recombinant protein (A) and lysate from mouse kidney (B) probed with EBI3 antibody at 0.5 and 5 ug/ml, respectively. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/EBI3-Antibody-5P6G8-Western-Blot-NBP2-03942-img0003.jpg "Western Blot: EBI3 Antibody (5P6G8) [NBP2-03942] - Analysis using EBI3 antibody. Mouse EBI recombinant protein (A) and lysate from mouse kidney (B) probed with EBI3 antibody at 0.5 and 5 ug/ml, respectively. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: EBI3 Antibody (5P6G8) [NBP2-03942] - Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 2 ug/10^6 cells.](http://images.novusbio.com/fullsize/EBI3-Antibody-5P6G8-Flow-Cytometry-NBP2-03942-img0002.jpg)
![Western Blot: EBI3 Antibody (10J706) [NBP2-03938] - Western blot testing of mouse EBI3 monoclonal antibody at 0.1 ug/ml on recombinant protein. Goat anti-rat Ig HRP secondary antibody and ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/EBI3-Antibody-10J706-Western-Blot-NBP2-03938-img0001.jpg "Western Blot: EBI3 Antibody (10J706) [NBP2-03938] - Western blot testing of mouse EBI3 monoclonal antibody at 0.1 ug/ml on recombinant protein. Goat anti-rat Ig HRP secondary antibody and ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-27/IL-35 EBI3 Subunit Antibody (10J706) [NBP2-03938] - EBI3 Antibody (10J706) [NBP2-03938] - Figure A: Intracellular analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10^6 cells. Figure B: Intracellular analysis of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using mouse EBI3 antibody at 1.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-10J706-Flow-Cytometry-NBP2-03938-img0004.jpg)