| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24767SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33220 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27345SS | |||
| Azide and BSA Free | NBP2-80793 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
![Western Blot: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - analysis of Integrin alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: MCF-7 cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Western-Blot-NBP2-67691-img0009.jpg "Western Blot: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - analysis of Integrin alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: MCF-7 cell lysateLane 2: A431 cell lysate")
![Immunocytochemistry/Immunofluorescence: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Staining ITGA2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunocytochemistry-Immunofluorescence-NBP2-67691-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded mouse stomach tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0008.jpg)
![Flow Cytometry: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of A431 cells with ITGA2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Flow-Cytometry-NBP2-67691-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Staining ITGA2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunocytochemistry-Immunofluorescence-NBP2-67691-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded human colon cancer tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded human kidney tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0006.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded mouse colon tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0007.jpg)
![Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP2-15811-img0006.jpg "Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50.")
![Flow Cytometry: CD45 Antibody [NBP2-15811] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4 C for 15 min. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488- Conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP2-15811-img0005.jpg)
![Western Blot: CD19 Antibody [NBP2-15782] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-Western-Blot-NBP2-15782-img0016.jpg "Western Blot: CD19 Antibody [NBP2-15782] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CD19 Antibody [NBP2-15782] - Human tonsil. CD19 stained by CD19 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD19-Antibody-Immunohistochemistry-Paraffin-NBP2-15782-img0011.jpg)
![Flow Cytometry: CD19 Antibody [NBP2-15782] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4-C for 15 min. Black: Unlabelled sample was used as a control. Red: CD19 antibody [C1C3] dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/CD19-Antibody-Flow-Cytometry-NBP2-15782-img0006.jpg)
![Western Blot: CD19 Antibody [NBP2-15782] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/CD19-Antibody-Western-Blot-NBP2-15782-img0007.jpg)
![CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunofluorescent analysis.Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD19 stained by CD19 antibody [C1C3] (NBP2-15782) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15782_rabbit-polyclonal-cd19-antibody-immunocytochemistry-immunofluorescence-261120251843192.jpg)
![CD19 antibody [C1C3] detects CD19 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human tonsil.Green: CD19 stained by CD19 antibody [C1C3] (NBP2-15782) diluted at 1:250.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15782_rabbit-polyclonal-cd19-antibody-immunohistochemistry-paraffin-2611202518393117.jpg)
![Flow Cytometry: TLR7 Antibody [NBP2-24767] - Intracellular staining by analysis of TLR7 in human PBMCs using this antibody at 2 ug/ml. Shaded histogrm represents cells alone; green represents rabbit IgG isotype control, and red represents the TLR7 antibody.](http://images.novusbio.com/fullsize/TLR7-Antibody-Flow-Cytometry-NBP2-24767-img0003.jpg "Flow Cytometry: TLR7 Antibody [NBP2-24767] - Intracellular staining by analysis of TLR7 in human PBMCs using this antibody at 2 ug/ml. Shaded histogrm represents cells alone; green represents rabbit IgG isotype control, and red represents the TLR7 antibody.")
![Western Blot: TLR7 Antibody [NBP2-24767] - Analysis of human TLR7 in Ramos cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody.](http://images.novusbio.com/fullsize/TLR7-Antibody-Western-Blot-NBP2-24767-img0001.jpg)
![Immunohistochemistry-Paraffin: TLR7 Antibody [NBP2-24767] - Human brain using 1:50 dilution of TLR7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Punctate staining of neurons was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TLR7-Antibody-Immunohistochemistry-Paraffin-NBP2-24767-img0005.jpg)
![Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Western-Blot-NBP2-19662-img0010.jpg "Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded human lung adenocarcinoma. p38 antibody [N1C3-2] diluted at 1:250.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0003.jpg)
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded rat brain. p38 MAPK antibody [N1C3-2] diluted at 1:500.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0002.jpg)
![p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-immunocytochemistry-immunofluorescence-261120251995418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-western-blot-261120251837242.jpg)
![Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Western-Blot-NB100-56532-img0007.jpg "Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: IKK gamma Antibody (46B844) [NB100-56532] - Human Jurkat cells were probed using 0.1 ug of NEMO antibody (red) and 0.1 ug of isotype control (green), with shaded histogram representing cells alone and an anti-mouse IgG-FITC secondary antibody.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Flow-Cytometry-NB100-56532-img0005.jpg)
![Western Blot: IRF3 Antibody [NBP1-78769] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), and NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-IRF3 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF3 was also immunoprecipitated by rabbit anti-IRF3 antibody NBP1-78768 which recognizes a disparate epitope.](http://images.novusbio.com/fullsize/IRF3-Antibody-Western-Blot-NBP1-78769-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: IRF3 Antibody [NBP1-78769] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), and NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-IRF3 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF3 was also immunoprecipitated by rabbit anti-IRF3 antibody NBP1-78768 which recognizes a disparate epitope.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0008.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Intracellular staining of Interleukin-1 beta positive T cells (upper panel) & monocytes (lower panel) from stimulated PBMC (with a cocktail of TLR ligands and PMA/IM for 6h) using Interleukin-1 beta antibody at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Flow-Cytometry-NBP2-27345-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of full length human IL-1 beta recombinant protein (10ng/well) using IL1 beta Antibody (NBP2-27345) at 1:1000 (left lane) and 1:100 (right lane).](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in human kidney tissue using Interleukin-1 beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Immunohistochemistry-Paraffin-NBP2-27345-img0007.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-Paraffin-NB110-93609-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.")
![Immunohistochemistry: CD45 Antibody (5C16) [NB110-93609] - CD45 antibody (5C16) [NB110-93609] immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-NB110-93609-img0001.jpg)
![Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609] - Correlation of MAP17 expression with inflammation in human samples of Crohn's disease & psoriasisMAP17, CD45 & CD68 staining of histological slides of human samples of psoriasis & Crohn's disease. MAP17 staining in both inflammatory diseases showed high expression levels, correlated with the inflammatory markers CD45 & CD68. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29228712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-93609_rat-monoclonal-cd45-antibody-5c16-310202415171311.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E (red) in FFPE lung carcinoma by IHC-IF. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunocytochemistry-Immunofluorescence-NBP2-76424-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E (red) in FFPE lung carcinoma by IHC-IF. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).")
![Western Blot: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E by WB of Jurkat, MOLT-4, K-562, SK-MEL-28 (SK), and HeLa lysate Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Western-Blot-NBP2-76424-img0003.jpg)
![Immunohistochemistry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunohistochemistry-NBP2-76424-img0001.jpg)
![Flow Cytometry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424) (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Flow-Cytometry-NBP2-76424-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in FFPE MOLT-4 cells (left) and Ramos cells (right) by ICC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunocytochemistry-NBP2-76424-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E (red) in FFPE appendix by IHC-IF. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] NBP2-76424. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunocytochemistry-Immunofluorescence-NBP2-76424-img0004.jpg)
![Immunoprecipitation: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunoprecipitation-NBP2-76424-img0007.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-simple-western-3032023112047.jpg "Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Zrecombinant monoclonal antibody [BL-336-1B2] (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Flow-Cytometry-NBP2-76399-img0007.jpg)
![Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinantmonoclonal antibody [BL-336-1B2] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-NBP2-76399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2].Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-Paraffin-NBP2-76399-img0009.jpg)
![Immunoprecipitation: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by WB of immunoprecipitates from Jurkat lysate.Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunoprecipitation-NBP2-76399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Rabbit antiCD247/CD3Z recombinant monoclonal antibody[BL-336-1B2]. (Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-immunohistochemistry-paraffin-732024101321..jpg)
![Western Blot: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Whole cell lysate (10 ug) from Jurkat, U2OS, SR, HEK293T, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-western-blot-732024101522..jpg)