| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70291 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71256 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76394-0.025mg | |||
![Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NB600-477-img0006.jpg "Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.")
![Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Paraffin-embedded mouse brain sections (IKK beta KO or IKK beta WT) were stained with IKK beta (green). Note: during staining, remember to add 0.1-0.3% Triton-100 in the antibody incubation buffer. See PMID 25253847 for further details regarding the experiemental conditions. Image from verified customer review.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NB600-477-img0003.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Human placenta tissue.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NB600-477-img0004.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser276] Antibody-Western Blot-NBP1-77807-img0010.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.")
![Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-NBP1-77807-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0005.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0008.jpg)
![Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0006.jpg "Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.")
![<b>Genetic Strategies Validation. </b>Western Blot: CEBP Delta Antibody [NB110-85519] - Detection of endogenous protein in whole cell extracts from mouse macrophage cells. Untreated WT cells (Lane 1), WT cells treated 3 hrs with LPS (Lane 2) and knock-out cells treated 3 hrs with LPS (Lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of endogenous C/EBP delta. Primary antibody at 1:15,000. ECL was used for detection.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 hrs with LPS. Lane 3: C/EBPd-knock-out cells treated 3 hrs with LPS. 35 ug per lane. Primary: anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0007.jpg)
![Western Blot: NLRC3 Antibody [NBP2-75593] - Analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Western-Blot-NBP2-75593-img0009.jpg "Western Blot: NLRC3 Antibody [NBP2-75593] - Analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0004.jpg)
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0008.jpg)
![Flow Cytometry: NLRC3 Antibody [NBP2-75593] - Analysis of MCF-7 cells with NLRC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Flow-Cytometry-NBP2-75593-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0003.jpg)
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0005.jpg)
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0006.jpg)
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0007.jpg)
![Western Blot: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: THP-1 cell lysate](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Western-Blot-NBP2-67700-img0004.jpg "Western Blot: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: THP-1 cell lysate")
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CCR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0005.jpg)
![Flow Cytometry: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of K562 cells with CCR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Flow-Cytometry-NBP2-67700-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CCR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18057-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP3-18057] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD4 antibody (NBP3-18057). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Paraffin-NBP3-18057-img0001.jpg)
![Flow Cytometry: CD4 Antibody [NBP3-18057] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP3-18057 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CD4-Antibody-Flow-Cytometry-NBP3-18057-img0003.jpg)
![Immunohistochemistry-Paraffin: Rabbit CD4 pAb [NBP3-18057] - Immunofluorescence staining of human tonsil FFPE tissue. Antibody diluted 1:50 (Catalog # NBP3-18057AF647) in 3% BSA and incubated over night at 4 °C. Heat mediated antigen retrieval at pH 9. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-18057_rabbit-cd4-pab-immunohistochemistry-paraffin-49202411280..jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32945-img0002.jpg "Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded rat spleen dilution: 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0010.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: BMDM (bone marrow-derived macrophage) cell B: Syk null cell 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0004.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0011.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human cervical cancer diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0008.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human endometrium diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0009.jpg)
![Immunoprecipitation: SYK Antibody [NBP1-32945] - Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 ug of SYK antibody. Western blot analysis was performed using SYK antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunoprecipitation-NBP1-32945-img0013.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody [N2C2], Internal (NBP1-32945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32945_rabbit-polyclonal-syk-antibody-western-blot-1411202518324112.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N2C2], Internal (NBP1-32945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32945_rabbit-polyclonal-syk-antibody-western-blot-261120251820234.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0010.jpg "Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: MIF Antibody [NBP1-32192] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32192-img0002.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - Paraffin-embedded mouse brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0012.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate)A: Molt-4 15% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0005.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - : Paraffin-embedded rat brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0011.jpg)
![ELISA: MIF Antibody [NBP1-32192] - ELISA detection of MIF [N1C3] capture at a concentration of 5 ug/mL and MIF C-term antibody for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-ELISA-NBP1-32192-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32192_rabbit-polyclonal-mif-antibody-western-blot-141120251840435.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32192_rabbit-polyclonal-mif-antibody-western-blot-1411202518443421.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded dog cerebellum. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249342769.jpg "Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded dog cerebellum. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-212024941415.jpg)
![Western Blot: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249432836.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded cat cerebellum. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249422675.jpg)
![Immunocytochemistry/Immunofluorescence: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-212024940844.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249425497.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-212024941481.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249393517.jpg)
![Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-western-blot-2611202518263616.jpg)
![Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Western-Blot-NBP2-02472-img0002.jpg "Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.")
![Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP2-02472-img0009.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded prostate tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0008.jpg)
![Flow Cytometry: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells transfected with either pCMV6-ENTRY BTK.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Flow-Cytometry-NBP2-02472-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] - Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP2-02472-img0003.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0004.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0005.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0006.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Kidney tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0007.jpg)
![Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Western-Blot-NBP1-48284-img0001.jpg "Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] Staining of COS7 cells transiently transfected by pCMV6-ENTRY JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0008.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human pancreas tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] - Immunofluorescent staining of HeLa cells using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0002.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Kidney tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0005.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Ovary tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0006.jpg)
![Western Blot: IL-33 Antibody [NBP1-76394] - Loading: 15ug of human lung lysate. Antibodies: IL-33, 2 g/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP1-76394-img0011.jpg "Western Blot: IL-33 Antibody [NBP1-76394] - Loading: 15ug of human lung lysate. Antibodies: IL-33, 2 g/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: IL-33 Antibody - BSA Free [NBP1-76394] - Validation of IL-33 in Human Lymph Node.Loading: 15. ug of human lymph node lysateAntibodies: IL-33, 2 ug/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76394_rabbit-polyclonal-il-33-antibody-2711202420141963.gif)
![Western Blot: IL-33 Antibody [NBP1-76394] - Analysis of IL-33 in A-20 cell lysate with IL-33 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP1-76394-img0006.jpg)
![Western Blot: IL-33 Antibody [NBP1-76394] - Loading: 5ng (1) and 25ng (2) of IL-33 partial human recombinant protein.Antibodies: IL-33, NBP1-76394, 1 ug/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Il-33 partial human recombinant protein: 112-270aa, 17.9kD, 36kD band is the dimer.](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP1-76394-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IL-33 Antibody [NBP1-76394] - Regulated Expression Validation of IL-33 in Human Skin Fibroblastsexpression of IL-33 in human skin fibroblasts following alpha-irradiation were detected by anti-IL-33 antibodies. IL-33 levels were increased after Alpha-irradiation, which were inhibited by treatment of PPP.](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP1-76394-img0009.jpg)
![Western Blot: IL-33 Antibody [NBP1-76394] - Human IL-33 has 4 isoforms, including isoform 1 (270aa, 30.8kD), isoform 2 (228aa, 25.9kD), isoform 3 (228aa, 26.1kD) and isoform 4 (144aa, 16.3kD). This antibody detects only human isoform L. Mouse IL-33 has 1 isoform (266aa, 30kD). Rat IL-33 has only one isoform identified so far (264aa, 29.5kD).](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP1-76394-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-33 Antibody [NBP1-76394] - Analysis of of IL-33 in human lymph node tissue with IL-33 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/IL-33-Antibody-Immunohistochemistry-Paraffin-NBP1-76394-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-33 Antibody - BSA Free [NBP1-76394] - Validation of IL-33 in Human Lymph Node.Immunofluorescent analysis of 4% paraformaldehyde-fixed human lymph node tissue labeling IL-33 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76394_rabbit-polyclonal-il-33-antibody-2711202419381748.jpg)