| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73398 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92538-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89913-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11882-25ug | |||
![Western Blot: PIAS2 Antibody (2B5) [NBP2-46394] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIAS2.](http://images.novusbio.com/fullsize/PIAS2-Antibody-2B5-Western-Blot-NBP2-46394-img0001.jpg "Western Blot: PIAS2 Antibody (2B5) [NBP2-46394] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIAS2.")
![Immunocytochemistry/Immunofluorescence: ZMYND11 Antibody [NB100-1157] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ZMYND11-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1157-img0001.jpg "Immunocytochemistry/Immunofluorescence: ZMYND11 Antibody [NB100-1157] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Western Blot: Adenosine A2b R Antibody [NBP1-68953] - Human breast cancer cell lines SUM149 and SUM159 were exposed to either 20% or 1% of oxygen and the expression of ADORA2B was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/Adenosine-A2b-R-Antibody-Western-Blot-NBP1-68953-img0004.jpg "Western Blot: Adenosine A2b R Antibody [NBP1-68953] - Human breast cancer cell lines SUM149 and SUM159 were exposed to either 20% or 1% of oxygen and the expression of ADORA2B was detected by Western blot. Image from verified customer review.")
![Western Blot: Adenosine A2b R Antibody [NBP1-68953] - Antibody at 0.2-1 ug/mL.](http://images.novusbio.com/fullsize/Adenosine-A2b-Receptor-Antibody-Western-Blot-NBP1-68953-img0003.jpg)
![Western Blot: C8B Antibody [NBP2-92538] - Analysis of extracts of various cell lines, using C8B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/C8B-Antibody-Western-Blot-NBP2-92538-img0001.jpg "Western Blot: C8B Antibody [NBP2-92538] - Analysis of extracts of various cell lines, using C8B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Flow Cytometry: Cystatin S Antibody (006) [NBP2-89913] - Analysis of Human CST4 expression on HeLa cells. The cells were treated, stained with purified anti-Human CST4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Cystatin-S-Antibody-006-Flow-Cytometry-NBP2-89913-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Cystatin S Antibody (006) [NBP2-89913] - Analysis of Human CST4 expression on HeLa cells. The cells were treated, stained with purified anti-Human CST4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cystatin S Antibody (006) [NBP2-89913] - Staining of Human CST4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89913 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89913_rabbit-monoclonal-cystatin-s-antibody-006-235202511444430.png)
![Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NBP1-44332] - Human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Adenosine-A2b-R-Antibody-Immunohistochemistry-Paraffin-NBP1-44332-img0003.jpg "Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NBP1-44332] - Human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NBP1-44332] - Analysis of anti-Adenosine A2b Receptor antibody with human brain, cortex.](http://images.novusbio.com/fullsize/Adenosine-A2b-R-Antibody-Immunohistochemistry-Paraffin-NBP1-44332-img0002.jpg)
![Western Blot: RFX1 Antibody [NBP1-52653] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RFX1 antibody used for IP at 6 mcg/mg lysate. RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies NBP1-52652 and NBP1-52654, which recognize other epitopes.](http://images.novusbio.com/fullsize/RFX1-Antibody-Western-Blot-NBP1-52653-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RFX1 Antibody [NBP1-52653] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RFX1 antibody used for IP at 6 mcg/mg lysate. RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies NBP1-52652 and NBP1-52654, which recognize other epitopes.")
![Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04026] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/NFATC3-NFAT4-Antibody-Immunoprecipitation-NBP2-04026-img0001.jpg "Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04026] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![Western Blot: NFATC3/NFAT4 Antibody [NBP2-04027] - Detection of human NFATc3 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04027 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04025 and NBP2-04026, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize/NFATC3-NFAT4-Antibody-Western-Blot-NBP2-04027-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NFATC3/NFAT4 Antibody [NBP2-04027] - Detection of human NFATc3 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04027 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04025 and NBP2-04026, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).")
![Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04025] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/NFATC3-NFAT4-Antibody-Immunoprecipitation-NBP2-04025-img0002.jpg "Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04025] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![Western Blot: ABP1/AOC1 Antibody (C12) [NBP3-41560] - 293T cell lysate Primary Ab: 3ug/ml NBP3-41560; Second Ab: 0.2ug/mL HRP-conjugated Goat Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41560_mouse-abp1-aoc1-mab-c12-141202515123717.jpg "Western Blot: ABP1/AOC1 Antibody (C12) [NBP3-41560] - 293T cell lysate Primary Ab: 3ug/ml NBP3-41560; Second Ab: 0.2ug/mL HRP-conjugated Goat Anti-Mouse IgG Polyclonal Antibody")
![Flow Cytometry: ABP1/AOC1 Antibody (C12) [NBP3-41560] - Human THP1 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100, then stained with 10ug/ml NBP3-41560 (red histogram) or Isotype control antibody (green histogram), followed by 1ug/ml FITC-conjugated Anti-mouse IgG Secondary Antibody](http://images.novusbio.com/fullsize/nbp3-41560_mouse-abp1-aoc1-mab-c12-141202515123710.jpg)