| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45080 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09044-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81083-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81084-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45082 | |||
| Azide and BSA Free | NBP2-34423-0.1mg | |||
![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (SPM508) - Azide and BSA Free [NBP2-34396] - Formalin-paraffin human tonsil stained with Kappa Light Chain MAb (SPM508). Note cell membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-SPM508-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34396-img0001.jpg "Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (SPM508) - Azide and BSA Free [NBP2-34396] - Formalin-paraffin human tonsil stained with Kappa Light Chain MAb (SPM508). Note cell membrane & cytoplasmic staining.")
![Western Blot: HLA F Antibody [NBP1-59537] - 721_B cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/HLA-F-Antibody-Western-Blot-NBP1-59537-img0002.jpg "Western Blot: HLA F Antibody [NBP1-59537] - 721_B cell lysate, concentration 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: HLA F Antibody [NBP1-59537] - Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Membrane and cytoplasmic in alveolar type I cells](http://images.novusbio.com/fullsize/HLA-F-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-59537-img0003.jpg)
![Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596] - Western blot analysis of extracts of Human plasma cells, using Kappa Light Chain antibody (NBP3-15596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-8O7Q9-Western-Blot-NBP3-15596-img0002.jpg "Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596] - Western blot analysis of extracts of Human plasma cells, using Kappa Light Chain antibody (NBP3-15596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596] - Immunohistochemistry of paraffin-embedded human tonsil using Kappa Light Chain Rabbit mAb (NBP3-15596) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-8O7Q9-Immunohistochemistry-Paraffin-NBP3-15596-img0001.jpg)
![Western Blot: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32514) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32514_rabbit-lag-3-mab-psh0-43-278202416331289.jpg "Western Blot: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32514) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32514) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32514_rabbit-lag-3-mab-psh0-43-298202414115425.jpg)
![Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-278202416285213.jpg "Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-2920241192322.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/LAG-3-Antibody-C9B7W-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09044-img0002.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![ELISA: LAG-3 Antibody (C9B7W) - Chimeric - Azide and BSA Free [NBP3-09044] - ELISA Plate coated with recombinant mouse LAG-3 Fc-Fusion Protein at a concentration of 2 ug/ml. A 4-fold serial dilution from 5,000 ng/ml was performed using NBP3-09044. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.](http://images.novusbio.com/fullsize/nbp3-09044_rabbit-monoclonal-lag-3-antibody-c9b7w-chimeric-74202522113011.png)
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-MEL-14-Flow-Cytometry-NBP2-81083-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) - Chimeric - Azide and BSA Free [NBP2-81083] - Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or NBP2-81083 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp2-81083_rabbit-monoclonal-l-selectin-cd62l-antibody-mel-14-74202522141216.png)
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG-56) - Chimeric [NBP2-81084] - Flow-cytometry using [NBP2-81084]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of DREG-56 (NBP2-81084, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG-56-Chimeric-Flow-Cytometry-NBP2-81084-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG-56) - Chimeric [NBP2-81084] - Flow-cytometry using [NBP2-81084]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of DREG-56 (NBP2-81084, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (KLC1278) [NBP2-54512] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Monoclonal Antibody (KLC1278).](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC1278-Immunohistochemistry-Paraffin-NBP2-54512-img0001.jpg "Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (KLC1278) [NBP2-54512] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Monoclonal Antibody (KLC1278).")
![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (SPM558) [NBP2-34376] - Formalin-paraffin human tonsil stained with Kappa Light Chain Ab (SPM558). Note cell membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-SPM558-Immunohistochemistry-Paraffin-NBP2-34376-img0001.jpg "Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (SPM558) [NBP2-34376] - Formalin-paraffin human tonsil stained with Kappa Light Chain Ab (SPM558). Note cell membrane & cytoplasmic staining.")