| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09044-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89590-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34902-1mg | |||
| Carrier-Free | NBP2-34902-100ug | |||
| Carrier-Free | NBP2-34902-500ug | |||
![Western Blot: GIMAP5 Antibody [NBP1-31165] - Sample (30 ug of whole cell lysate)A: HeLa S3 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-Western-Blot-NBP1-31165-img0002.jpg "Western Blot: GIMAP5 Antibody [NBP1-31165] - Sample (30 ug of whole cell lysate)A: HeLa S3 12% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: GIMAP5 Antibody [NBP1-31165] - Paraffin-embedded breast cancer. GIMAP5 antibody dilution: 1:250.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-Immunohistochemistry-Paraffin-NBP1-31165-img0003.jpg)
![Western Blot: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32514) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32514_rabbit-lag-3-mab-psh0-43-278202416331289.jpg "Western Blot: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32514) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PSH0-43) [NBP3-32514] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32514) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32514) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32514_rabbit-lag-3-mab-psh0-43-298202414115425.jpg)
![Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-278202416285213.jpg "Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-2920241192322.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/LAG-3-Antibody-C9B7W-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09044-img0002.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![ELISA: LAG-3 Antibody (C9B7W) - Chimeric - Azide and BSA Free [NBP3-09044] - ELISA Plate coated with recombinant mouse LAG-3 Fc-Fusion Protein at a concentration of 2 ug/ml. A 4-fold serial dilution from 5,000 ng/ml was performed using NBP3-09044. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.](http://images.novusbio.com/fullsize/nbp3-09044_rabbit-monoclonal-lag-3-antibody-c9b7w-chimeric-74202522113011.png)
![Western Blot: Roquin Antibody [NB100-656] - Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-656 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). Roquin was also immunoprecipitated using rabbit anti-Roquin antibody NB100-655 at 3 ug/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-656-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Roquin Antibody [NB100-656] - Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-656 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). Roquin was also immunoprecipitated using rabbit anti-Roquin antibody NB100-655 at 3 ug/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Sandwich ELISA: GIMAP5 Antibody (1E10) [H00055340-M10] - Detection limit for recombinant GST tagged GIMAP5 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-1E10-Sandwich-ELISA-H00055340-M10-img0002.jpg "Sandwich ELISA: GIMAP5 Antibody (1E10) [H00055340-M10] - Detection limit for recombinant GST tagged GIMAP5 is 1 ng/ml as a capture antibody.")
![Immunoprecipitation: GIMAP5 Antibody (1E10) [H00055340-M10] - Analysis of GIMAP5 transfected lysate using anti-GIMAP5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GIMAP5 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-1E10-Immunoprecipitation-H00055340-M10-img0001.jpg)
![Western Blot: GIMAP5 Antibody [H00055340-B01P] - Analysis of GIMAP5 expression in human spleen.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-Western-Blot-H00055340-B01P-img0004.jpg "Western Blot: GIMAP5 Antibody [H00055340-B01P] - Analysis of GIMAP5 expression in human spleen.")
![Western Blot: GIMAP5 Antibody [H00055340-B01P] - Analysis of GIMAP5 expression in Jurkat.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-Western-Blot-H00055340-B01P-img0003.jpg)
![Western Blot: GIMAP5 Antibody [H00055340-B01P] - Analysis of GIMAP5 expression in transfected 293T cell line by GIMAP5 polyclonal antibody. Lane 1: GIMAP5 transfected lysate(33.77 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-Western-Blot-H00055340-B01P-img0005.jpg)
![Immunohistochemistry: Roquin Antibody [NBP1-89590] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.](http://images.novusbio.com/fullsize/Roquin-Antibody-Immunohistochemistry-NBP1-89590-img0003.jpg "Immunohistochemistry: Roquin Antibody [NBP1-89590] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.")
![Western Blot: Galectin-8 Antibody (3E5) [H00003964-M01] - LGALS8 monoclonal antibody (M01), clone 3E5. Analysis of LGALS8 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-3E5-Western-Blot-H00003964-M01-img0003.jpg "Western Blot: Galectin-8 Antibody (3E5) [H00003964-M01] - LGALS8 monoclonal antibody (M01), clone 3E5. Analysis of LGALS8 expression in Raw 264.7.")
![ELISA: Galectin-8 Antibody (3E5) [H00003964-M01] - Galectin-8 Antibody (3E5) [H00003964-M01] - Detection limit for recombinant GST tagged LGALS8 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Galectin-8-Antibody-3E5-ELISA-H00003964-M01-img0002.jpg)
![Western Blot: Coronin-1a Overexpression Lysate (Adult Normal) [NBL1-09395] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09395-img0002.jpg "Western Blot: Coronin-1a Overexpression Lysate (Adult Normal) [NBL1-09395] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Coronin-1a.")
![SDS-Page: Human IL-7 Protein [NBP2-34902] - MW= Approximately 17 kDa](http://images.novusbio.com/fullsize/Human-IL-7-Protein-SDS-Page-NBP2-34902-img0001.jpg "SDS-Page: Human IL-7 Protein [NBP2-34902] - MW= Approximately 17 kDa")