| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11969SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48450 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48450 | |||
| Azide and BSA Free | NBP2-54336-100ug | |||
![Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/OX40-Ligand-TNFSF4-Antibody-MM0505-8S23-Flow-Cytometry-NBP2-11969-img0001.jpg "Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Immunohistochemistry-Paraffin: CD86 Antibody (AP-MAB0803) [NBP2-12182] - 4% PFA fixed and paraffin embedded mouse lung tissue (LPS treated) was subjected to IHC staining using this CD86](http://images.novusbio.com/fullsize/CD86-Antibody-AP-MAB0803-Immunohistochemistry-Paraffin-NBP2-12182-img0001.jpg "Immunohistochemistry-Paraffin: CD86 Antibody (AP-MAB0803) [NBP2-12182] - 4% PFA fixed and paraffin embedded mouse lung tissue (LPS treated) was subjected to IHC staining using this CD86")
![Flow Cytometry: HLA G Antibody (G233) [NB110-55298] - Surface staining of HLA-G in HLA-G transfectants using anti-HLA-G (G233) purified / GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-G233-Flow-Cytometry-NB110-55298-img0001.jpg "Flow Cytometry: HLA G Antibody (G233) [NB110-55298] - Surface staining of HLA-G in HLA-G transfectants using anti-HLA-G (G233) purified / GAM-APC.")
![Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).](http://images.novusbio.com/fullsize/HLA-G-Antibody-2A12-Flow-Cytometry-NBP1-44925-img0002.jpg "Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).")
![Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells) with anti-HLA-G (2A12) purified, GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-2A12-Flow-Cytometry-NBP1-44925-img0001.jpg)
![Western Blot: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 ug/Lane) Lane 2: NCI-H226 cell lysate (20 ug/Lane) Lane 3: Human brain tissue lysate (30 ug/Lane) Lane 4: Human placenta tissue lysate (30 ug/Lane) Lane 5: Human lung tissue lysate (30 ug/Lane) Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32132) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-278202415295558.jpg "Western Blot: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 ug/Lane) Lane 2: NCI-H226 cell lysate (20 ug/Lane) Lane 3: Human brain tissue lysate (30 ug/Lane) Lane 4: Human placenta tissue lysate (30 ug/Lane) Lane 5: Human lung tissue lysate (30 ug/Lane) Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32132) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD200/OX2 with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32132, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-298202414333642.jpg)
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32132) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-298202414304113.jpg)
![Flow Cytometry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Flow cytometric analysis of SK-MEL-28 cells labeling CD200/OX2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32132, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-29202416453219.jpg)
![Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-EM-05-Flow-Cell-Surface-NBP1-44763-img0002.jpg "Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.")
![Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Western-Blot-NBP3-09029-img0001.jpg "Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 (NBP3-09029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09029-img0002.jpg)
![Flow Cytometry: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free [NBP3-09029] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09029 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-09029_rabbit-monoclonal-hvem-tnfrsf14-antibody-hmhv-1b18-chimeric-74202522141222.png)
![Western Blot: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/1131).](http://images.novusbio.com/fullsize/CD45RA-Antibody-PTPRC-1131-Azide-and-BSA-Free-Western-Blot-NBP2-54336-img0002.jpg "Western Blot: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/1131).")
![Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336] - Formalin-fixed paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (PTPRC/1131).](http://images.novusbio.com/fullsize/CD45RA-Antibody-PTPRC-1131-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54336-img0001.jpg)
![Western Blot: CD45RA Antibody (PTPRC/1131) [NBP2-44845] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/1131).](http://images.novusbio.com/fullsize/CD45RA-Antibody-PTPRC-1131-Western-Blot-NBP2-44845-img0002.jpg "Western Blot: CD45RA Antibody (PTPRC/1131) [NBP2-44845] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/1131).")
![Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1131) [NBP2-44845] - Human Tonsil stained with CD45RA Monoclonal Antibody (PTPRC/1131).](http://images.novusbio.com/fullsize/CD45RA-Antibody-PTPRC-1131-Immunohistochemistry-Paraffin-NBP2-44845-img0001.jpg)
![Western Blot: CD3 epsilon Antibody [NBP2-32282] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Western-Blot-NBP2-32282-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CD3 epsilon Antibody [NBP2-32282] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-32282] - Sample: FFPE section of human lung cancer infiltrate. Antibody: Affinity purified rabbit anti- CD3E used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-32282-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CD3 epsilon Antibody [NBP2-32282] - Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for IP at 6 ug per reaction. CD3E was also immunoprecipitated by rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (NBP2076424) and another rabbit anti-CD3E antibody. For blotting immunoprecipitated CD3E, NBP2-32282 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 10 seconds.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunoprecipitation-NBP2-32282-img0005.jpg)