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![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [Alexa Fluor 488] [NBP1-47518AF488] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Calreticulin (1G6A7) conjugated to Alexa Fluor 488 [NBP1-47518AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Alexa-Fluor-488-Immunocytochemistry-Immunofluorescence-NBP1-47518AF488-img0007.jpg "Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [Alexa Fluor 488] [NBP1-47518AF488] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Calreticulin (1G6A7) conjugated to Alexa Fluor 488 [NBP1-47518AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Flow Cytometry: Calreticulin Antibody (1G6A7) [Alexa Fluor® 488] [NBP1-47518AF488] - An intracellular stain was performed on HepG2 cells with Calreticulin Antibody (1G6A7) NBP1-47518AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Alexa-Fluor-R-488-Flow-Cytometry-NBP1-47518AF488-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [Alexa Fluor 488] [NBP1-47518AF488] - The Calreticulin antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Alexa-Fluor-488-Immunocytochemistry-Immunofluorescence-NBP1-47518AF488-img0006.jpg)
