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![Western Blot: proBNP Antibody (50E1) [NB200-440] - Analysis using the Biotin conjugate of NB200-440. Detection of synthetic huBNP in WB after Tricine-SDS-PAGE in reducing conditions.](http://images.novusbio.com/fullsize/proBNP-Antibody-50E1-Western-Blot-NB200-440-img0006.jpg "Western Blot: proBNP Antibody (50E1) [NB200-440] - Analysis using the Biotin conjugate of NB200-440. Detection of synthetic huBNP in WB after Tricine-SDS-PAGE in reducing conditions.")
![Western Blot: proBNP Antibody (50E1) [NB200-440] - Immunodetection of human synthetic BNP (A), recombinant proBNP (E. coli) (B) and recombinant proBNP (mammalian cell line) (C) in Western blotting by different monoclonal antibodies after Tricine- SDS-PAGE under reducing conditions. A, B. Lanes: 1- MAb 24C511-17, 2- MAb 26E211-22, 3- MAb 2G9, 4- MAb 43B1226-32, 5- MAb 50B726-32, 6- MAb 50E126-32, 7- MAb 57H326-32.](http://images.novusbio.com/fullsize/proBNP-Antibody-50E1-Western-Blot-NB200-440-img0004.jpg)
![Immunoassay: proBNP Antibody (50E1) [NB200-440] - Gel-filtration studies of endogenous proBNP and BNP. BNP immunoreactivity measurements in fractions after proteins from plasma of HF patients were separated by gel-filtration on Superdex Peptide column (GEHealthcare). One representative plasma sample. BNP immunoreactivity was measured by 50E126-32-24C511-17 assay (- -). The first peak corresponds to proBNP, the second corresponds to BNP form.](http://images.novusbio.com/fullsize/proBNP-Antibody-50E1-Immunoassay-NB200-440-img0003.jpg)
![Western Blot: NT-proBNP Antibody (13G12) [NB120-14712] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards (Bio-Rad) Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250](http://images.novusbio.com/fullsize/proBNP-Antibody-13G12-Western-Blot-NB120-14712-img0003.jpg "Western Blot: NT-proBNP Antibody (13G12) [NB120-14712] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards (Bio-Rad) Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250")
![ELISA: NT-proBNP Antibody (13G12) [NB120-14712] - Calibration curve for NT-proBNP 15C4-13G12 assay using NT-proBNP Antibody as a detection antibody: 13G1213-20 (Eu3+ - labeled) and biotinylated capture antibody. Antigen: human recombinant NT-proBNP expressed in E. coli](http://images.novusbio.com/fullsize/proBNP-Antibody-13G12-ELISA-NB120-14712-img0007.jpg)
![Sandwich ELISA: NT-proBNP Antibody (13G12) [NB120-14712] - Stability studies of endogenous NT-proBNP being measured in sandwich immunoassay.](http://images.novusbio.com/fullsize/proBNP-Antibody-13G12-Sandwich-ELISA-NB120-14712-img0006.jpg)
![Western Blot: BNP Antibody (JE30-94) [NBP3-32088] - Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (NBP3-32088) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: U-2 OS cell lysate (20 ug/Lane) Lane 4: Mouse brain tissue lysate (30 ug/Lane) Lane 5: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 15 kDa Observed band size: 37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32088_rabbit-bnp-mab-je30-94-278202415242679.jpg "Western Blot: BNP Antibody (JE30-94) [NBP3-32088] - Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (NBP3-32088) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: U-2 OS cell lysate (20 ug/Lane) Lane 4: Mouse brain tissue lysate (30 ug/Lane) Lane 5: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 15 kDa Observed band size: 37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: BNP Antibody (JE30-94) [NBP3-32088] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-BNP antibody (NBP3-32088) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32088) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32088_rabbit-bnp-mab-je30-94-29820241492684.jpg)
![Immunohistochemistry-Paraffin: BNP Antibody (NPPB/4493) [NBP3-13984] - Formalin-fixed, paraffin-embedded human uterusstained with BNP antibody (NPPB/4493). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/BNP-Antibody-NPPB-4493-Immunohistochemistry-Paraffin-NBP3-13984-img0002.jpg "Immunohistochemistry-Paraffin: BNP Antibody (NPPB/4493) [NBP3-13984] - Formalin-fixed, paraffin-embedded human uterusstained with BNP antibody (NPPB/4493). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: BNP Antibody (NPPB/4493) [NBP3-13984] - Formalin-fixed, paraffin-embedded human heart stained with BNP antibody (NPPB/4493). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/BNP-Antibody-NPPB-4493-Immunohistochemistry-Paraffin-NBP3-13984-img0001.jpg)
![Protein Array: BNP Antibody (NPPB/4493) [NBP3-13984] - Analysis of Protein Array containing more than 19,000 full-length human proteins using BNP Antibody (NPPB/4493).](http://images.novusbio.com/fullsize/BNP-Antibody-NPPB-4493-Protein-Array-NBP3-13984-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: proBNP Antibody [NBP1-32038] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BNP protein stained by BNP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/proBNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32038-img0006.jpg "Immunocytochemistry/Immunofluorescence: proBNP Antibody [NBP1-32038] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BNP protein stained by BNP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Western Blot: BNP Antibody [NBP1-32038] - BNP antibody detects BNP protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BNP antibody (NBP1-32038) diluted by 1:1000.](http://images.novusbio.com/fullsize/nbp1-32038_rabbit-polyclonal-bnp-antibody-91020242011445.jpg)
![Western Blot: ORAI3 Antibody (S06-8B3) [NBP3-19901] - Western blot detection of ORAI3 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19901 (1:1000 diluted). Predicted band size: 32kDa. Observed band size: 32kDa.](http://images.novusbio.com/fullsize/ORAI3-Antibody-S06-8B3-Western-Blot-NBP3-19901-img0002.jpg "Western Blot: ORAI3 Antibody (S06-8B3) [NBP3-19901] - Western blot detection of ORAI3 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19901 (1:1000 diluted). Predicted band size: 32kDa. Observed band size: 32kDa.")
![Immunohistochemistry-Paraffin: ORAI3 Antibody (S06-8B3) [NBP3-19901] - Immunohistochemistry of ORAI3 in paraffin-embedded liver cancer tissue using NBP3-19901 at dilution 1/50](http://images.novusbio.com/fullsize/ORAI3-Antibody-S06-8B3-Immunohistochemistry-Paraffin-NBP3-19901-img0001.jpg)
![Western Blot: NPPB Antibody (2D11) [H00004879-M01] - Analysis of NPPB expression in transfected 293T cell line by NPPB monoclonal antibody (M01), clone 2D11.Lane 1: NPPB transfected lysate(14.726 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NPPB-Antibody-2D11-Western-Blot-H00004879-M01-img0004.jpg "Western Blot: NPPB Antibody (2D11) [H00004879-M01] - Analysis of NPPB expression in transfected 293T cell line by NPPB monoclonal antibody (M01), clone 2D11.Lane 1: NPPB transfected lysate(14.726 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: NPPB Antibody (2D11) [H00004879-M01] - Analysis of NPPB transfected lysate using anti-NPPB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NPPB MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NPPB-Antibody-2D11-Immunoprecipitation-H00004879-M01-img0003.jpg)
![Western Blot: proBNP Antibody (11D1) [NB120-13059] - Detection of human recombinant proBNP expressed in mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 29D125-12; 3 - 15F1113-24; 4 - 13G1213-20; 5 - 18H513-20; 6 - 16F313-20; 7 - 11D131-39; 8 - 15D748-56; 9 - 15C463-71; 10 - 24E1167-76. Antigen: recombinant proBNP expressed in mammalian cells 0.15 ug/line. MAbs 11D131-39, 15D748-56, 15C463-71 and 24E1167-76 does not recognize glycosylated proBNP expressed in the mammalian cell line.](http://images.novusbio.com/fullsize/proBNP-Antibody-11D1-Western-Blot-NB120-13059-img0012.jpg "Western Blot: proBNP Antibody (11D1) [NB120-13059] - Detection of human recombinant proBNP expressed in mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 29D125-12; 3 - 15F1113-24; 4 - 13G1213-20; 5 - 18H513-20; 6 - 16F313-20; 7 - 11D131-39; 8 - 15D748-56; 9 - 15C463-71; 10 - 24E1167-76. Antigen: recombinant proBNP expressed in mammalian cells 0.15 ug/line. MAbs 11D131-39, 15D748-56, 15C463-71 and 24E1167-76 does not recognize glycosylated proBNP expressed in the mammalian cell line.")
![Western Blot: proBNP Antibody (11D1) [NB120-13059] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards (Bio-Rad) Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250.](http://images.novusbio.com/fullsize/proBNP-Antibody-11D1-Western-Blot-NB120-13059-img0004.jpg)
![Western Blot: proBNP Antibody (11D1) [NB120-13059] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 - 24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.](http://images.novusbio.com/fullsize/proBNP-Antibody-11D1-Western-Blot-NB120-13059-img0013.jpg)
![Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 - 24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.](http://images.novusbio.com/fullsize/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0009.jpg "Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 - 24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.")
![Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards. Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250.](http://images.novusbio.com/fullsize/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0011.jpg)
![Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant proBNP expressed in mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells 0.15 ug/line. MAbs 7, 8, 9 and 10 do not recognize glycosylated proBNP expressed in the mammalian cell line.](http://images.novusbio.com/fullsize/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0010.jpg)
![ELISA: Rat NT-proBNP ELISA Kit (Chemiluminescence) [NBP2-68139] - Samples were spiked with high concentrations of Rat NT-proBNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68139_rat-nt-probnp-elisa-kit-chemiluminescence-132202416204095.jpg "ELISA: Rat NT-proBNP ELISA Kit (Chemiluminescence) [NBP2-68139] - Samples were spiked with high concentrations of Rat NT-proBNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat NT-proBNP ELISA Kit (Chemiluminescence) [NBP2-68139] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68139_rat-nt-probnp-elisa-kit-chemiluminescence-432024856760.jpg)
![Western Blot: Orai2 Antibody (8D11) [NBP2-46295] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ORAI2.](http://images.novusbio.com/fullsize/Orai2-Antibody-8D11-Western-Blot-NBP2-46295-img0001.jpg "Western Blot: Orai2 Antibody (8D11) [NBP2-46295] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ORAI2.")
![Western Blot: proBNP Antibody (5B6) [NB120-13111] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 -24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.](http://images.novusbio.com/fullsize/proBNP-Antibody-5B6-Western-Blot-NB120-13111-img0008.jpg "Western Blot: proBNP Antibody (5B6) [NB120-13111] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 -24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.")
![Western Blot: proBNP Antibody (5B6) [NB120-13111] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards (Bio-Rad) Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250](http://images.novusbio.com/fullsize/proBNP-Antibody-5B6-Western-Blot-NB120-13111-img0003.jpg)