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![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2B11-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12838-img0004.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.")
![Immunohistochemistry: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2B11-VHH-Immunohistochemistry-NBP3-12838-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1 ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2B11-VHH-Flow-Cytometry-NBP3-12838-img0003.jpg)
![ELISA: LAG-3 Antibody (2B11) - VHH [NBP3-12838] - Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2B11-VHH-ELISA-NBP3-12838-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1C6-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12839-img0004.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.")
![Immunohistochemistry: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1C6-VHH-Immunohistochemistry-NBP3-12839-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1 ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1C6-VHH-Flow-Cytometry-NBP3-12839-img0003.jpg)
![ELISA: LAG-3 Antibody (1C6) - VHH [NBP3-12839] - Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.](http://images.novusbio.com/fullsize/LAG-3-Antibody-1C6-VHH-ELISA-NBP3-12839-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2A11-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12836-img0004.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 ug/mL.")
![Immunohistochemistry: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2A11-VHH-Immunohistochemistry-NBP3-12836-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1 ug/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2A11-VHH-Flow-Cytometry-NBP3-12836-img0003.jpg)
![ELISA: LAG-3 Antibody (2A11) - VHH [NBP3-12836] - Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.](http://images.novusbio.com/fullsize/LAG-3-Antibody-2A11-VHH-ELISA-NBP3-12836-img0001.jpg)
![Western Blot: LAG-3 Antibody (OTI8F6) [NBP2-71112] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3.](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Azide-and-BSA-Free-Western-Blot-NBP2-71112-img0001.jpg "Western Blot: LAG-3 Antibody (OTI8F6) [NBP2-71112] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3.")
![Immunohistochemistry: LAG-3 Antibody (OTI8F6) - Azide and BSA Free [NBP2-71112] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71112-img0003.jpg)
![Flow Cytometry: LAG-3 Antibody (OTI8F6) - Azide and BSA Free [NBP2-71112] - Analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-LAG3 antibody (1:100).](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71112-img0002.jpg)
![Immunohistochemistry: LAG-3 Antibody (OTI8F6) - Azide and BSA Free [NBP2-71112] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71112-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with transfected cells expressing Histamine receptor H3 at 18 ug/ml.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunocytochemistry-Immunofluorescence-NLS476-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with transfected cells expressing Histamine receptor H3 at 18 ug/ml.")
![Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with human brain, neurons and glia at 18 ug/ml.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunohistochemistry-Paraffin-NLS476-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histamine H3 R Antibody [NLS476] - Analysis of anti-HRH3 / Histamine H3 Receptor antibody with untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunocytochemistry-Immunofluorescence-NLS476-img0002.jpg)
![Immunohistochemistry-Paraffin: CXCR1/IL-8 RA Antibody [NLS806] - Analysis of anti-CXCR1 antibody with human lymph node, Hodgkins lymphoma.](http://images.novusbio.com/fullsize/CXCR1-IL-8-RA-Antibody-Immunohistochemistry-Paraffin-NLS806-img0008.jpg "Immunohistochemistry-Paraffin: CXCR1/IL-8 RA Antibody [NLS806] - Analysis of anti-CXCR1 antibody with human lymph node, Hodgkins lymphoma.")
![Immunohistochemistry-Paraffin: CXCR1/IL-8RA Antibody [NLS806] - Staining of untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/CXCR1-IL-8-RA-Antibody-Immunohistochemistry-Paraffin-NLS806-img0005.jpg)
![Immunohistochemistry-Paraffin: CXCR1/IL-8RA Antibody [NLS806] - Staining of HEK293 human embryonic kidney cells transfected with CXCR1.](http://images.novusbio.com/fullsize/CXCR1-IL-8-RA-Antibody-Immunohistochemistry-Paraffin-NLS806-img0007.jpg)
![Immunohistochemistry-Paraffin: CXCR1/IL-8RA Antibody [NLS806] - Human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CXCR1-IL-8-RA-Antibody-Immunohistochemistry-Paraffin-NLS806-img0003.jpg)
![Flow Cytometry: Histamine H1R Antibody [NB600-795] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Histamine-H1R-Antibody-Flow-Cytometry-NB600-795-img0005.jpg "Flow Cytometry: Histamine H1R Antibody [NB600-795] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS3262] - Analysis of anti-Histamine 3 Receptor / HRH3 antibody with human brain, neurons and glia at dilution 7-15 ug/ml.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunohistochemistry-Paraffin-NLS3262-img0005.jpg "Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS3262] - Analysis of anti-Histamine 3 Receptor / HRH3 antibody with human brain, neurons and glia at dilution 7-15 ug/ml.")
![Immunohistochemistry-Paraffin: Histamine H3 R Antibody [NLS3262] - Analysis of anti-Histamine 3 Receptor / HRH3 antibody with human brain, neurons.](http://images.novusbio.com/fullsize/Histamine-H3-R-Antibody-Immunohistochemistry-Paraffin-NLS3262-img0003.jpg)
![Western Blot: LAG-3 Antibody (PS01-33) [NBP3-32516] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/1,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 57/70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-2882024142261.jpg "Western Blot: LAG-3 Antibody (PS01-33) [NBP3-32516] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/1,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 57/70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PS01-33) [NBP3-32516] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32516) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-29820241416185.jpg)
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PS01-33) [NBP3-32516] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG-3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-29202410334064.jpg)