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![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0002.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.")
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Detection of endogenous human APRIL with MAb to APRIL (human) (Aprily-5). Method: Cell lysate (A) or cell supernatant (B) were run on an SDS-PAGE gel and analyzed with 2ug/ml Aprily-5 by Western blot. Dendritic cells (1), NIH3T3 (2) and NIH3T3 expressing human APRIL (3).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0001.jpg)
![Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-5A8-Flow-Cytometry-NBP1-97639-img0001.jpg "Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.")
![Western Blot: BCMA/TNFRSF17 Antibody (Vicky-2) [NBP1-97608] - Lane 1: MW marker, Lane 2: Mouse BCMA Recombinant Protein.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-2-Western-Blot-NBP1-97608-img0002.jpg "Western Blot: BCMA/TNFRSF17 Antibody (Vicky-2) [NBP1-97608] - Lane 1: MW marker, Lane 2: Mouse BCMA Recombinant Protein.")
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0002.jpg "Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).")
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Surface staining pattern of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0001.jpg)
![Western Blot: APRIL/TNFSF13 Antibody (SR1578) [NBP3-22069] - Western blot analysis using NBP3-22069 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22069_rabbit-april-tnfsf13-mab-sr1578-5720231558489.jpg "Western Blot: APRIL/TNFSF13 Antibody (SR1578) [NBP3-22069] - Western blot analysis using NBP3-22069 in Jurkat cell lysate.")
![Immunocytochemistry/Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-H4-C7-Immunocytochemistry-Immunofluorescence-NBP1-04265-img0004.jpg "Immunocytochemistry/Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)")
![Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - Flow cytometry analysis of BLyS in PBMC cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-H4-C7-Flow-Cytometry-NBP1-04265-img0006.jpg)
![ELISA: Rat BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric) [NBP2-69925] - Samples were spiked with high concentrations of Rat BAFF/BLyS/TNFSF13B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69925_rat-baff-blys-tnfsf13b-elisa-kit-colorimetric-132202416204066.jpg "ELISA: Rat BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric) [NBP2-69925] - Samples were spiked with high concentrations of Rat BAFF/BLyS/TNFSF13B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat BAFF/BLyS/TNFSF13B ELISA Kit (Colorimetric) [NBP2-69925] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69925_rat-baff-blys-tnfsf13b-elisa-kit-colorimetric-432024941517.jpg)
![ELISA: Human BAFFR/TNFRSF13C ELISA Kit (Colorimetric) [NBP2-69926] - Samples were spiked with high concentrations of Human BAFFR/TNFRSF13C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69926_human-baffr-tnfrsf13c-elisa-kit-colorimetric-132202416221026.jpg "ELISA: Human BAFFR/TNFRSF13C ELISA Kit (Colorimetric) [NBP2-69926] - Samples were spiked with high concentrations of Human BAFFR/TNFRSF13C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human BAFFR/TNFRSF13C ELISA Kit (Colorimetric) [NBP2-69926] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69926_human-baffr-tnfrsf13c-elisa-kit-colorimetric-432024932639.jpg)
![Immunohistochemistry-Paraffin: BAFFR/TNFRSF13C Antibody (BAFFR/1558) [NBP3-07544] - Formalin-fixed, paraffin-embedded human tonsil stained with BAFFR/TNFRSF13C Mouse Monoclonal Antibody (BAFFR/1558).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-BAFFR-1558-Immunohistochemistry-Paraffin-NBP3-07544-img0001.jpg "Immunohistochemistry-Paraffin: BAFFR/TNFRSF13C Antibody (BAFFR/1558) [NBP3-07544] - Formalin-fixed, paraffin-embedded human tonsil stained with BAFFR/TNFRSF13C Mouse Monoclonal Antibody (BAFFR/1558).")