| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77284-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99343-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90437-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
![Western Blot: DC-SIGN/CD209 Antibody [NBP1-77284] - Detection of DC-SIGN in human small intestine at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-Western-Blot-NBP1-77284-img0001.jpg "Western Blot: DC-SIGN/CD209 Antibody [NBP1-77284] - Detection of DC-SIGN in human small intestine at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: DC-SIGN/CD209 Antibody - BSA Free [NBP1-77284] - Immunofluorescence of DC-SIGN/CD209 in Human Lymph Node tissue with DC-SIGN/CD209 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77284_rabbit-polyclonal-dc-sign-cd209-antibody-2711202419392790.jpg)
![Immunohistochemistry: DC-SIGN/CD209 Antibody - BSA Free [NBP1-77284] - Immunohistochemistry of DC-SIGN/CD209 in human lymph node tissue with DC-SIGN/CD209 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77284_rabbit-polyclonal-dc-sign-cd209-antibody-271120242013293.jpg)
![Western Blot: SR-AI/MSR Antibody (10O8B0) [NBP3-16126] - Western blot analysis of extracts of various cell lines, using SR-AI/MSR antibody (NBP3-16126) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-10O8B0-Western-Blot-NBP3-16126-img0003.jpg "Western Blot: SR-AI/MSR Antibody (10O8B0) [NBP3-16126] - Western blot analysis of extracts of various cell lines, using SR-AI/MSR antibody (NBP3-16126) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (10O8B0) [NBP3-16126] - Immunohistochemistry of paraffin-embedded mouse testis using SR-AI/MSR Rabbit mAb (NBP3-16126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-10O8B0-Immunohistochemistry-Paraffin-NBP3-16126-img0002.jpg)
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (10O8B0) [NBP3-16126] - Immunohistochemistry of paraffin-embedded rat brain using SR-AI/MSR Rabbit mAb (NBP3-16126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-10O8B0-Immunohistochemistry-Paraffin-NBP3-16126-img0001.jpg)
![Western Blot: TIRAP Antibody (9T1A8) [NBP3-16854] - Western blot analysis of extracts of various cell lines, using TIRAP Rabbit mAb (NBP3-16854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Western-Blot-NBP3-16854-img0003.jpg "Western Blot: TIRAP Antibody (9T1A8) [NBP3-16854] - Western blot analysis of extracts of various cell lines, using TIRAP Rabbit mAb (NBP3-16854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: TIRAP Antibody (9T1A8) [NBP3-16854] - Immunohistochemistry of paraffin-embedded mouse kidney using TIRAP Rabbit mAb (NBP3-16854) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Immunohistochemistry-Paraffin-NBP3-16854-img0002.jpg)
![Immunohistochemistry-Paraffin: TIRAP Antibody (9T1A8) [NBP3-16854] - Immunohistochemistry of paraffin-embedded rat lung using TIRAP Rabbit mAb (NBP3-16854) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Immunohistochemistry-Paraffin-NBP3-16854-img0001.jpg)
![Western Blot: FXR1 Antibody [NBP2-99343] - Anti-FXR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-99343-img0004.jpg "Western Blot: FXR1 Antibody [NBP2-99343] - Anti-FXR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 72 kDa")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP2-99343] - Immunochemical staining of human FXR1 in human brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP2-99343-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP2-99343] - Immunofluorescence staining of FXR1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99343 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99343_rabbit-polyclonal-fxr1-antibody-23520251129368.png)
![Western Blot: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32165) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-278202415295560.jpg "Western Blot: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32165) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32165) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-29820241433363.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32165) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-2920241048480.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32162) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29820241435295.jpg "Immunohistochemistry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32162) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunocytochemistry analysis of THP-1 cells labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32162) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29202410334075.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Flow cytometric analysis of THP-1 cells labeling CD68/SR-D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32162, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29202416453272.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 1 minute 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-278202415212467.jpg "Western Blot: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 1 minute 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32164) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-298202414161818.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32164) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-29202410234268.jpg)
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (004) [NBP2-90437] - Immunochemical staining of mouse SR-AI/MSR in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-004-Immunohistochemistry-Paraffin-NBP2-90437-img0003.jpg "Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (004) [NBP2-90437] - Immunochemical staining of mouse SR-AI/MSR in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).")
![Flow Cytometry: SR-AI/MSR Antibody (004) [NBP2-90437] - Flow cytometric analysis of Mouse SR-AI/MSR expression on Raw264.7 cells. Cells were stained with purified anti-Mouse SR-AI/MSR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-004-Flow-Cytometry-NBP2-90437-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody (004) [NBP2-90437] - Confocal immunofluorescence analysis of Mouse SR-AI/MSR1 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90437 antibody (1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/nbp2-90437_rabbit-monoclonal-sr-ai-msr-antibody-004-235202511444470.png)
![Western Blot: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: NCI-H226 cell lysate Lane 3: Human brain tissue lysate Lane 4: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-278202415212462.jpg "Western Blot: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: NCI-H226 cell lysate Lane 3: Human brain tissue lysate Lane 4: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32133) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-298202413585577.jpg)
![Flow Cytometry: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Flow cytometric analysis of SK-MEL-28 cells labeling CD200/OX2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-392024884632.jpg)
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-IFNG-466-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54394-img0003.jpg "Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)")
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-IFNG-466-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54394-img0002.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with IFN-gamma Mouse Monoclonal Antibody (IFNG/466).](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-IFNG-466-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54394-img0005.jpg)