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![Western Blot: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Western blot analysis of Complement Component C1qA on human kidney tissue lysates with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32097) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32097_rabbit-complement-component-c1qa-mab-je66-59-278202415242688.jpg "Western Blot: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Western blot analysis of Complement Component C1qA on human kidney tissue lysates with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32097) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32097) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32097_rabbit-complement-component-c1qa-mab-je66-59-29820241411543.jpg)
![Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32456_rabbit-icam-2-cd102-mab-je63-28-278202416285280.jpg "Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32456) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32456_rabbit-icam-2-cd102-mab-je63-28-298202414262629.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-RFB4-Flow-Cytometry-NBP2-49890-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0002.jpg "Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with feline leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (1B4) - Chimeric [NBP3-09040] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of 1B4 (NBP3-09040) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09040, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-1B4-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09040-img0002.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (1B4) - Chimeric [NBP3-09040] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of 1B4 (NBP3-09040) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09040, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Integrin beta 2/CD18 Antibody (1B4) - Chimeric [NBP3-09040] - Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 1B4 (NBP3-09040, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-1B4-Chimeric-Flow-Cytometry-NBP3-09040-img0001.jpg)
![Western Blot: RASA4 Antibody [NBP2-15089] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/RASA4-Antibody-Western-Blot-NBP2-15089-img0002.jpg "Western Blot: RASA4 Antibody [NBP2-15089] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: RASA4 Antibody [NBP2-15089] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RASA4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15089-img0001.jpg)
![Western Blot: HLA B Antibody [NBP2-14983] - Sample (30 ug of whole cell lysate) A: HL-60 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/HLA-B-Antibody-Western-Blot-NBP2-14983-img0002.jpg "Western Blot: HLA B Antibody [NBP2-14983] - Sample (30 ug of whole cell lysate) A: HL-60 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: HLA B Antibody [NBP2-14983] - Mahlarvu xenograft, using HLA-B antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HLA-B-Antibody-Immunohistochemistry-Paraffin-NBP2-14983-img0001.jpg)
![Western Blot: Complement C4a Antibody [NBP2-14893] - Sample (30 ug of whole cell lysate) A: HepG2; 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Complement-C4a-Antibody-Western-Blot-NBP2-14893-img0003.jpg "Western Blot: Complement C4a Antibody [NBP2-14893] - Sample (30 ug of whole cell lysate) A: HepG2; 5% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: Complement C4a Antibody [NBP2-14893] - Human hepatoma, using C4 antibody [N1N2-2], N-term antibody at 1:200 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Complement-C4a-Antibody-Immunohistochemistry-Paraffin-NBP2-14893-img0001.jpg)
![Western Blot: FMNL1 Antibody [NBP1-33431] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FMNL1-Antibody-Western-Blot-NBP1-33431-img0003.jpg "Western Blot: FMNL1 Antibody [NBP1-33431] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: FMNL1 Antibody [NBP1-33431] - Paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/FMNL1-Antibody-Immunohistochemistry-Paraffin-NBP1-33431-img0002.jpg)
![Western Blot: T-bet/TBX21 Antibody [NBP2-20583] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Western-Blot-NBP2-20583-img0003.jpg "Western Blot: T-bet/TBX21 Antibody [NBP2-20583] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:3000.")
![Immunohistochemistry-Paraffin: T-bet/TBX21 Antibody [NBP2-20583] - DLD-1 xenograft, using TBX21 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Immunohistochemistry-Paraffin-NBP2-20583-img0004.jpg)