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![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0002.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.")
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Detection of endogenous human APRIL with MAb to APRIL (human) (Aprily-5). Method: Cell lysate (A) or cell supernatant (B) were run on an SDS-PAGE gel and analyzed with 2ug/ml Aprily-5 by Western blot. Dendritic cells (1), NIH3T3 (2) and NIH3T3 expressing human APRIL (3).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (GL2A7) [NBP1-97499] - Mouse astrocytes stained with Anti-LAMP-2/CD107b. Cells were fixed using paraformaldehyde-lysine-periodate (including 4% paraformaldehyde) and permeabilized using 0.01% saponin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-GL2A7-Immunocytochemistry-Immunofluorescence-NBP1-97499-img0008.jpg "Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (GL2A7) [NBP1-97499] - Mouse astrocytes stained with Anti-LAMP-2/CD107b. Cells were fixed using paraformaldehyde-lysine-periodate (including 4% paraformaldehyde) and permeabilized using 0.01% saponin.")
![Immunocytochemistry/Immunofluorescence: Integrin alpha 2b/CD41 Antibody (IVA30) [NB500-496] - Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (thrombocyte-enriched suspension) using anti-bovine CD41/CD61 (IVA30); secondary antibody conjugated to FITC (green signal at thrombocytes), DNA stained with DAPI (blue signal).](http://images.novusbio.com/fullsize/Integrin-alpha-2b-CD41-Antibody-IVA30-Immunocytochemistry-Immunofluorescence-NB500-496-img0001.jpg "Immunocytochemistry/Immunofluorescence: Integrin alpha 2b/CD41 Antibody (IVA30) [NB500-496] - Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (thrombocyte-enriched suspension) using anti-bovine CD41/CD61 (IVA30); secondary antibody conjugated to FITC (green signal at thrombocytes), DNA stained with DAPI (blue signal).")
![Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Analysis using the FITC conjugate of NB500-376. Staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD15-Lewis-X-Antibody-MEM-158-Flow-Cytometry-NB500-376-img0003.jpg "Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Analysis using the FITC conjugate of NB500-376. Staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.")
![Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Surface staining (mass cytometry) of human peripheral blood cells (after ammonium chloride red blood cell lysis) with anti-human CD15 (MEM-158) 173Yb. Gated on singlets.](http://images.novusbio.com/fullsize/CD15-Lewis-X-Antibody-MEM-158-Flow-Cytometry-NB500-376-img0001.jpg)
![Western Blot: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/1,000 dilution. Lane 1: L-929 whole cell lysate Lane 2: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours whole cell lysate Lane 3: L-929 treated with pp for 1 hour whole cell lysate Lane 4: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours, treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32795) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32795_rabbit-ripk3-rip3-p-ser232-mab-je44-62-288202412442911.jpg "Western Blot: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/1,000 dilution. Lane 1: L-929 whole cell lysate Lane 2: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours whole cell lysate Lane 3: L-929 treated with pp for 1 hour whole cell lysate Lane 4: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours, treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32795) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Immunocytochemistry analysis of L-929 cells treated with or without 20μM Z-VAD for 2.75 hours, add 100nM SM-164 and 20ng/ml TNF-alpha for 2.25 hours, then treated with lambda pp for 1 hour labeling RIPK3/RIP3 with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32795_rabbit-ripk3-rip3-p-ser232-mab-je44-62-29202410234210.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-RFB4-Flow-Cytometry-NBP2-49890-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).")
![Western Blot: CD14 Antibody (PD01-63) [NBP3-32125] - Western blot analysis of CD14 on different lysates with Rabbit anti-CD14 antibody (NBP3-32125) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32125) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32125_rabbit-cd14-mab-pd01-63-278202416285230.jpg "Western Blot: CD14 Antibody (PD01-63) [NBP3-32125] - Western blot analysis of CD14 on different lysates with Rabbit anti-CD14 antibody (NBP3-32125) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32125) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD14 Antibody (PD01-63) [NBP3-32125] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CD14 antibody (NBP3-32125) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32125) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32125_rabbit-cd14-mab-pd01-63-29820241492696.jpg)
![Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr142-Antibody-EM-54-Western-Blot-NBP2-37708-img0001.jpg "Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5")
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Separation of human Integrin alpha 5/CD49e positive monocytes (red-filled) from human Integrin alpha 5/CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-SAM1-Flow-Cytometry-NBP3-18529-img0002.jpg "Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Separation of human Integrin alpha 5/CD49e positive monocytes (red-filled) from human Integrin alpha 5/CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).")
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-SAM1-Flow-Cytometry-NBP3-18529-img0001.jpg)
![Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73556] - Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Monkey-IgM-Heavy-Chain-Secondary-Antibody-[Biotin]-Western-Blot-NBP1-73556-img0003.jpg "Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73556] - Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None.")
![Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73379] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Rat-IgM-Secondary-Antibody-[Rhodamine]-Western-Blot-NBP1-73379-img0004.jpg "Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73379] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")