| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-61018-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98484-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90185-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98267-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90660-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50328-0.025ml | |||
![Immunohistochemistry-Paraffin: VEGF Receptor 3 Antibody (RM0003-5F63) [NB110-61018] - 4% PFA fixed mouse heart and kidney tissue sections were subjected to IHC with rat anti mouse VEGFR3 (RM0003-5F63).](http://images.novusbio.com/fullsize/VEGF-Receptor-3-Antibody-RM0003-5F63-Immunohistochemistry-Paraffin-NB110-61018-img0001.jpg "Immunohistochemistry-Paraffin: VEGF Receptor 3 Antibody (RM0003-5F63) [NB110-61018] - 4% PFA fixed mouse heart and kidney tissue sections were subjected to IHC with rat anti mouse VEGFR3 (RM0003-5F63).")
![Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-278202415212460.jpg "Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Immunocytochemistry analysis of 293T cells labeling ERK3/MAPK6 with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-2920241192388.jpg)
![Flow Cytometry: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Flow cytometric analysis of 293T cells labeling ERK3/MAPK6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32567, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-392024884681.jpg)
![Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Western-Blot-NBP2-67191-img0002.jpg "Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western Blot: DEC1 Antibody (PSH01-90) [NBP3-32946] - Western blot analysis of 45627 on different lysates with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 46/50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-288202412442966.jpg "Western Blot: DEC1 Antibody (PSH01-90) [NBP3-32946] - Western blot analysis of 45627 on different lysates with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 46/50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunohistochemical analysis of paraffin-embedded human bladder tissue with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32946) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-298202413585575.jpg)
![Immunocytochemistry/ Immunofluorescence: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunocytochemistry analysis of MCF7 cells labeling DEC1 with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-29202410234280.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)
![Western Blot: GBL Antibody [NBP2-98484] - Anti-GBL rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/GBL-Antibody-Western-Blot-NBP2-98484-img0004.jpg "Western Blot: GBL Antibody [NBP2-98484] - Anti-GBL rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: GBL Antibody [NBP2-98484] - Immunochemical staining of human GBL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunohistochemistry-Paraffin-NBP2-98484-img0003.jpg)
![Immunoprecipitation: GBL Antibody [NBP2-98484] - GBL was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 ul anti-GBL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GBL rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/GBL-Antibody-Immunoprecipitation-NBP2-98484-img0005.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0003.jpg "Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human squamous cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody (001) [NBP2-90185] - Staining of RRM2B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90185 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90185_rabbit-monoclonal-p53r2-antibody-001-235202514492316.png)
![Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Lane A: Mouse liver tissue lysateLane B: Mouse skeletal muscle tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Western-Blot-NBP2-98267-img0005.jpg "Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Lane A: Mouse liver tissue lysateLane B: Mouse skeletal muscle tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa")
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse skeletal muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0004.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse heart with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0002.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0003.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0003.jpg "Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0001.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunofluorescence staining of P4HB in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90660 antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-90660_rabbit-monoclonal-protein-disulfide-isomerase-p4hb-antibody-023-235202514492359.png)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Western blot analysis of Carbonic Anhydrase II/CA2 on different lysates with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32105) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-278202415242692.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Western blot analysis of Carbonic Anhydrase II/CA2 on different lysates with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32105) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32105) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29820241420924.jpg)
![Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunocytochemistry analysis of THP-1 cells labeling Carbonic Anhydrase II/CA2 with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29202410334053.jpg)