| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75903SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29626SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48532-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81696-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70954 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93587-0.02ml | |||
![Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-Immunohistochemistry-NBP2-75903-img0002.jpg "Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.")
![ELISA: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - ELISA Plate coated with human EGFR-Fc at a concentration of 2.0 mg/ml. A 2-fold serial dilution from 100 to 0.1 ng/ml was performed using HRP labelled cetuximab human IgG1 (NBP2-75903).](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-ELISA-NBP2-75903-img0001.jpg)
![Western Blot: SNAIL Antibody [NBP2-29626] - Western blot analysis of SNAIL in HeLa whole cell extract.](http://images.novusbio.com/fullsize/SNAIL-Antibody-Western-Blot-NBP2-29626-img0001.jpg "Western Blot: SNAIL Antibody [NBP2-29626] - Western blot analysis of SNAIL in HeLa whole cell extract.")
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0022.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NBP2-16895-img0015.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0019.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0020.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0021.jpg)
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 alpha antibody (NBP2-16895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16895_rabbit-polyclonal-hsp90-alpha-antibody-91020241957223.jpg)
![Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0003.jpg "Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.")
![Western Blot: ATM Antibody [NB100-678] - MDA-MB-231 and MCF-7 whole cell lysates were loaded with 50 ug/lane. 8% SDS-PAGE. ATM Antibody (NB100-678) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0009.jpg)
![Immunohistochemistry: ATM Antibody [NB100-678] - FFPE section of human prostate-nodular hypertrophy. Affinity purified rabbit [NB100-678] used at a dilution of 1:1000 ( 1ug/mL). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ATM-Antibody-Immunohistochemistry-NB100-678-img0007.jpg)
![Western Blot: ATM Antibody [NB100-678] - Analysis in whole cell lysates from human lung cancer lines with [NB100-678]. Theoretical molecular weight 351 kDa. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0008.jpg)
![Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Western-Blot-NBP2-27208-img0002.jpg "Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P < 0.05, **P < 0.01 & ***P < 0.001, two-tailed Student’s t-test. Unprocessed original scans of blots are shown in Supplementary Fig. 5. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-17376-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-31020241612213.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HCT116 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091458.jpg)
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at cytoplasm and nucleus by immunofluorescent analysis.<br>Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.<br>Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.<br>Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse colon.</br>MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.<br />IP samples: Jurkat whole cell extract<br />A. 30 ug Jurkat cell whole cell extract<br />B. Control with 4 ug of preimmune Rabbit IgG<br />C. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)<br />7.5 % SDS-PAGE<br />The immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.<br />[EasyBlot anti-rabbit IgG was used as a secondary reagent]<br />](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of purified antibody to P4HB on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-H00005034-B01P-img0005.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in HeLa.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0006.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in transfected 293T cell line by P4HB polyclonal antibody. Lane 1: P4HB transfected lysate(55.88 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0008.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in human liver.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0007.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Absence of TRIC-B alters expression of collagen-interacting proteins.(A) Quantitative RT-PCR of fibroblast transcripts encoding collagen, modifying enzymes & chaperones. Proband cells (P1, P2, & P3) demonstrate decreased expression of COL1A1 & FKBP10 (FKBP65), & increased expression of PLOD1 (LH1) & HSPA5 (BiP/GRP78) versus control fibroblasts (C). (B) Decreased expression of PLOD2 (LH2), FKBP10 (FKBP65) & PPIB (CyPB) in P2 osteoblasts versus control osteoblasts (C). (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes & chaperones in proband & control cells. Proband fibroblasts show increases in PDI, LH1, LH2 & BiP protein levels. CyPB & FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts & osteoblasts. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006156), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005034-b01p_mouse-polyclonal-protein-disulfide-isomerase-p4hb-antibody-31020241548512.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cell line Hep G2 shows localization to lipid droplets. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48532-img0006.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cell line Hep G2 shows localization to lipid droplets. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Western Blot-Perilipin-2/ADFP Antibody [NBP2-48532] -Analysis in human cell lines RT-4 and U-251MG using Anti-PLIN2 antibody. Corresponding PLIN2 RNA-seq data are presented for the same cell lines.](http://images.novusbio.com/fullsize/antibody/nbp2-48532_rabbit-polyclonal-perilipin-2-adfp-antibody-western-blot-285202412135..jpg)
![Western Blot: HspA4 Antibody [NBP1-81696] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/HspA4-Antibody-Western-Blot-NBP1-81696-img0005.jpg "Western Blot: HspA4 Antibody [NBP1-81696] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: HspA4 Antibody [NBP1-81696] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HspA4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81696-img0007.jpg)
![Immunohistochemistry-Paraffin: HspA4 Antibody [NBP1-81696] - Staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/HspA4-Antibody-Immunohistochemistry-Paraffin-NBP1-81696-img0006.jpg)
![Western Blot: HspA4 Antibody [NBP1-81696] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/HspA4-Antibody-Western-Blot-NBP1-81696-img0004.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Western-Blot-NBP2-02190-img0011.jpg "Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)")
![Immunocytochemistry/Immunofluorescence: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunocytochemistry-Immunofluorescence-NBP2-02190-img0008.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Flow-Cytometry-NBP2-02190-img0010.jpg)
![Western Blot: Hsp70 Antibody (3G9) [NBP2-02190] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Western-Blot-NBP2-02190-img0009.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0004.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0005.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0006.jpg)
![Western Blot: PER2 Antibody [NBP2-93587] - Analysis of extracts of 293T cells, using PER2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NBP2-93587-img0002.jpg "Western Blot: PER2 Antibody [NBP2-93587] - Analysis of extracts of 293T cells, using PER2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-93587] - Mouse testis using PER2 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-93587-img0005.jpg)
![Chromatin Immunoprecipitation: PER2 Antibody [NBP2-93587] - Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using PER2 antibody (NBP2-93587) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/PER2-Antibody-Chromatin-Immunoprecipitation-NBP2-93587-img0006.jpg)
![Western Blot: PER2 Antibody [NBP2-93587] - Analysis of extracts of Mouse liver, using PER2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NBP2-93587-img0001.jpg)
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-93587] - Rat spleen using PER2 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-93587-img0003.jpg)
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-93587] - Human thyroid cancer using PER2 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-93587-img0004.jpg)
![Immunohistochemistry-Frozen: Rabbit Polyclonal PER2 Antibody [NBP2-93587] - 10X magnification. Immunoreactive cell bodies (DAB staining) along the 4th ventricle in 20um from Hyla cinerea. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-93587_rabbit-polyclonal-per2-antibody-immunohistochemistry-frozen-510202311487..png)
