| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12019-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26050 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26012 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26024 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12018-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric [NBP3-12019] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of KM-2 (NBP3-12019) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12019, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-ac-Lys16-ac-Lys8-Antibody-KM-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12019-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric [NBP3-12019] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of KM-2 (NBP3-12019) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12019, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric - Azide and BSA Free [NBP3-12019] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12019 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12019_rabbit-monoclonal-histone-h4-ac-lys12-ac-lys16-ac-lys8-antibody-km-2-chimeric-74202522113023.png)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Western-Blot-NBP2-42859-img0003.jpg "Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42859-img0002.jpg)
![Western Blot: HA Tag Antibody (RMH02) [NBP2-61476] - Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61476, followed by a HRP conjugated anti-Human IgG secondary antibody.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RMH02-Western-Blot-NBP2-61476-img0003.jpg "Western Blot: HA Tag Antibody (RMH02) [NBP2-61476] - Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61476, followed by a HRP conjugated anti-Human IgG secondary antibody.")
![ELISA: HA Tag Antibody (RMH02) [NBP2-61476] - Sandwich ELISA performed on whole lysate of LNCaP cells overexpressing HA-tag Bag1 protein. ELISA plate was coated with chimeric human anti-HA-Tag antibody Clone NBP2-61476 as the capture antibody. Anti-Bag1 rabbit serum was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RMH02-ELISA-NBP2-61476-img0002.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26012_rabbit-histone-h4-mab-rm204-azide-and-bsa-free-2462024132637.png "Western Blot: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - RM204 specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26012_rabbit-histone-h4-mab-rm204-azide-and-bsa-free-22620242311017.png)
![Western Blot: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61551, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-RM204-Western-Blot-NBP2-61551-img0004.jpg "Western Blot: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61551, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - NBP2-61551 specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-RM204-Multiplex-Immunoassay-NBP2-61551-img0005.jpg)
![Western Blot: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using NBP2-61552, showed a band of Histone H4 trimethylated at Lysine 20 (K20me3) in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-Methyl-Lys20-Antibody-RM208-Western-Blot-NBP2-61552-img0004.jpg "Western Blot: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using NBP2-61552, showed a band of Histone H4 trimethylated at Lysine 20 (K20me3) in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - NBP2-61552 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) when used at higher concentrations. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.](http://images.novusbio.com/fullsize/Histone-H4-Methyl-Lys20-Antibody-RM208-Multiplex-Immunoassay-NBP2-61552-img0005.jpg)
![Western Blot: HA Tag Antibody (C.15200190) [NBP2-59172] - Whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the antibody directed against HA at 1:1,000. Figure shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-C-15200190-Western-Blot-NBP2-59172-img0001.jpg "Western Blot: HA Tag Antibody (C.15200190) [NBP2-59172] - Whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the antibody directed against HA at 1:1,000. Figure shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.")
![Western Blot: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K12ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-C-15200218-Western-Blot-NBP2-59241-img0001.jpg "Western Blot: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K12ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K12ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-C-15200218-Chromatin-Immunoprecipitation-NBP2-59241-img0002.jpg)