| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23624SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05341-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62560-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62559-0.025mg | |||
![Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0002.jpg "Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Untreated (-) and treated (+) Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0001.jpg)
![Western Blot: Frizzled-7 Antibody [NBP2-23624] - analysis of mouse Fz7 in pancreas lysate with anti-fz7 at a 1:500 dilution.](http://images.novusbio.com/fullsize/Frizzled-7-Antibody-Western-Blot-NBP2-23624-img0001.jpg "Western Blot: Frizzled-7 Antibody [NBP2-23624] - analysis of mouse Fz7 in pancreas lysate with anti-fz7 at a 1:500 dilution.")
![Western Blot: CSN7b Antibody [NB100-461] - Detection of Human CSN7b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSN7b antibody NB100-461 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CSN7b-Antibody-Western-Blot-NB100-461-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: CSN7b Antibody [NB100-461] - Detection of Human CSN7b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSN7b antibody NB100-461 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: CSN7b Antibody [NB100-461] - Whole cell lysate (50 ug - E; 10 ug - T) from HEK293T cells with (T) or without (E) with a CSN7b expression construct. Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/CSN7b-Antibody-Western-Blot-NB100-461-img0003.jpg)
![Western Blot: SRGAP1 Antibody [NB100-60650] - Detection of Human SRGAP1/ARHGAP13 on HeLa whole cell lysate using NB100-60650. SRGAP1/ARHGAP13 was also IPed by NB100-60649.](http://images.novusbio.com/fullsize/SRGAP1-Antibody-Western-Blot-NB100-60650-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SRGAP1 Antibody [NB100-60650] - Detection of Human SRGAP1/ARHGAP13 on HeLa whole cell lysate using NB100-60650. SRGAP1/ARHGAP13 was also IPed by NB100-60649.")
![Western Blot: AKT2 Antibody [NBP3-05341] - Western blot analysis of extracts of various cell lines, using AKT2 antibody (NBP3-05341) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/AKT2-Antibody-Western-Blot-NBP3-05341-img0001.jpg "Western Blot: AKT2 Antibody [NBP3-05341] - Western blot analysis of extracts of various cell lines, using AKT2 antibody (NBP3-05341) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: AKT2 Antibody - Azide and BSA Free [NBP3-05341] - Western blot analysis of lysates from 293T cells, using AKT2 Rabbit pAb (A18019) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-05341_mouse-polyclonal-akt2-antibody-52202519302971.jpg)
![Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Numb-Antibody-9X1Y0-Western-Blot-NBP3-16738-img0001.jpg "Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunoprecipitation: Numb Antibody (9X1Y0) [NBP3-16738] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Numb antibody (NBP3-16738). Western blot was performed from the immunoprecipitate using Numb (NBP3-16738) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Numb-Antibody-9X1Y0-Immunoprecipitation-NBP3-16738-img0002.jpg)
![Western Blot: Notch-1 Antibody (7L3V4) [NBP3-15658] - Western blot analysis of extracts of various cell lines, using Notch-1 antibody (NBP3-15658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Notch-1-Antibody-7L3V4-Western-Blot-NBP3-15658-img0001.jpg "Western Blot: Notch-1 Antibody (7L3V4) [NBP3-15658] - Western blot analysis of extracts of various cell lines, using Notch-1 antibody (NBP3-15658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunoprecipitation: Notch-1 Antibody (7L3V4) [NBP3-15658] - Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug Notch-1 antibody (NBP3-15658). Western blot was performed from the immunoprecipitate using Notch-1 antibody (NBP3-15658) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Notch-1-Antibody-7L3V4-Immunoprecipitation-NBP3-15658-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B9) - Chimeric [NBP2-62560] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B9. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B9 (NBP2-62560) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62560, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Notch-2-Antibody-B9-Chimeric-Immunofluorescence-NBP2-62560-img0001.jpg "Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B9) - Chimeric [NBP2-62560] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B9. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B9 (NBP2-62560) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62560, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B6) - Chimeric [NBP2-62559] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B6 (NBP2-62559) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62559, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Notch-2-Antibody-B6-Chimeric-Immunofluorescence-NBP2-62559-img0001.jpg "Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B6) - Chimeric [NBP2-62559] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B6 (NBP2-62559) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62559, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: Artemin Antibody [NBP1-68857] - (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Artemin-Antibody-Western-Blot-NBP1-68857-img0003.jpg "Western Blot: Artemin Antibody [NBP1-68857] - (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PHOX2A Antibody [NBP2-26140] - NBP2-26140 (1ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-Western-Blot-NBP2-26140-img0002.jpg "Western Blot: PHOX2A Antibody [NBP2-26140] - NBP2-26140 (1ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")