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![Western Blot: GOLGB1/Giantin Antibody [NBP2-22323] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22323 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Western-Blot-NBP2-22323-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: GOLGB1/Giantin Antibody [NBP2-22323] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22323 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22323] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22323 used for IP at 6 ug/mg lysate. Giantin was also immunoprecipitated by rabbit anti-Giantin antibodies NBP2-22321 and NBP2-22322. For blotting immunoprecipitated Giantin, NBP2-22323 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunoprecipitation-NBP2-22323-img0003.jpg)
![Immunohistochemistry:[NBP3-30575]-Detection of human GM130 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GM130 used at a dilution of 1: 200. Detection: DAB. Counterstain: hematoxylin.](http://images.novusbio.com/fullsize/nbp3-30575_rabbit-gm130-golga2-pab-23120251204074.jpg "Immunohistochemistry:[NBP3-30575]-Detection of human GM130 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GM130 used at a dilution of 1: 200. Detection: DAB. Counterstain: hematoxylin.")
![Western Blot: Notch-1 Antibody (7L3V4) [NBP3-15658] - Western blot analysis of extracts of various cell lines, using Notch-1 antibody (NBP3-15658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Notch-1-Antibody-7L3V4-Western-Blot-NBP3-15658-img0001.jpg "Western Blot: Notch-1 Antibody (7L3V4) [NBP3-15658] - Western blot analysis of extracts of various cell lines, using Notch-1 antibody (NBP3-15658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunoprecipitation: Notch-1 Antibody (7L3V4) [NBP3-15658] - Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug Notch-1 antibody (NBP3-15658). Western blot was performed from the immunoprecipitate using Notch-1 antibody (NBP3-15658) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Notch-1-Antibody-7L3V4-Immunoprecipitation-NBP3-15658-img0002.jpg)
![Western Blot: Albumin Antibody (3O4L7) [NBP3-15415] - Analysis of extracts of various cell lines, using Bovine Serum Albumin / BSA Rabbit mAb (NBP3-15415) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Albumin-Antibody-3O4L7-Western-Blot-NBP3-15415-img0001.jpg "Western Blot: Albumin Antibody (3O4L7) [NBP3-15415] - Analysis of extracts of various cell lines, using Bovine Serum Albumin / BSA Rabbit mAb (NBP3-15415) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.")
![Western Blot: Furin Antibody (1P6N8) [NBP3-16535] - Western blot analysis of extracts of various cell lines, using Furin Rabbit mAb (NBP3-16535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Furin-Antibody-1P6N8-Western-Blot-NBP3-16535-img0001.jpg "Western Blot: Furin Antibody (1P6N8) [NBP3-16535] - Western blot analysis of extracts of various cell lines, using Furin Rabbit mAb (NBP3-16535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: Furin Antibody (1P6N8) [NBP3-16535] - Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug Furin antibody (NBP3-16535). Western blot was performed from the immunoprecipitate using Furin antibody (NBP3-16535) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Furin-Antibody-1P6N8-Immunoprecipitation-NBP3-16535-img0002.jpg)
![SDS-Page: Transferrin Native Protein [NBP1-97006] - Mouse Transferrin Lane 1: Mouse Transferrin Load: 1.0 ug per lane Predicted/Observed size: 80 kDa, 80 kDa](http://images.novusbio.com/fullsize/Transferrin-Native-Protein-SDS-Page-NBP1-97006-img0001.jpg "SDS-Page: Transferrin Native Protein [NBP1-97006] - Mouse Transferrin Lane 1: Mouse Transferrin Load: 1.0 ug per lane Predicted/Observed size: 80 kDa, 80 kDa")
![Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. System was used for signal detection (Roche) using a 60-sec exposure time.](http://images.novusbio.com/fullsize/Gamma Adaptin Antibody-Western Blot-NB110-7877-img0003.jpg "Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. System was used for signal detection (Roche) using a 60-sec exposure time.")
![Western Blot: Human Serum Albumin Antibody [NBP2-21669] - Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin.](http://images.novusbio.com/fullsize/Human-Serum-Albumin-Antibody-Western-Blot-NBP2-21669-img0001.jpg "Western Blot: Human Serum Albumin Antibody [NBP2-21669] - Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin.")
![Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B6) - Chimeric [NBP2-62559] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B6 (NBP2-62559) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62559, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Notch-2-Antibody-B6-Chimeric-Immunofluorescence-NBP2-62559-img0001.jpg "Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B6) - Chimeric [NBP2-62559] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B6 (NBP2-62559) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62559, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B9) - Chimeric [NBP2-62560] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B9. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B9 (NBP2-62560) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62560, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Notch-2-Antibody-B9-Chimeric-Immunofluorescence-NBP2-62560-img0001.jpg "Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B9) - Chimeric [NBP2-62560] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B9. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B9 (NBP2-62560) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62560, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP1-06049] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and lipid droplet staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06049-img0001.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP1-06049] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and lipid droplet staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")