| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26494-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50122-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57867-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98334-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99780-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22361-50ul | |||
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-262202415355749.jpg "Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-27220248313715.jpg)
![Flow Cytometry: ATM Antibody (3G11) [NBP3-26494] - Overlay histogram showing Hela cells stained with ATM Antibody (3G11) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-282202414312732.jpg)
![Immunocytochemistry/Immunofluorescence: ATM Antibody [NBP2-57867] - Staining of human cell line U-2 OS using ATM Antibody [NBP2-57867] shows no positivity.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57867-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATM Antibody [NBP2-57867] - Staining of human cell line U-2 OS using ATM Antibody [NBP2-57867] shows no positivity.")
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-324-img0003.jpg "Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324] - Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-BLM antibody NB100-324 (lot NB100-324-2) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot NB100-324-1) and rabbit anti-BLM antibody NB100-214. For blotting immunoprecipitated BLM, NB100-324 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunoprecipitation-NB100-324-img0005.jpg)
![Western Blot: FANCL Antibody [NBP1-49839] - Analysis of FANCL in Human Bone Marrow lysate (35ug protein in RIPA buffer) NBP1-49839 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/FANCL-Antibody-Western-Blot-NBP1-49839-img0002.jpg "Western Blot: FANCL Antibody [NBP1-49839] - Analysis of FANCL in Human Bone Marrow lysate (35ug protein in RIPA buffer) NBP1-49839 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48729_rabbit-blooms-syndrome-protein-blm-mab-hl2771-western-blot-1411202518392011.jpg "<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48729_rabbit-blooms-syndrome-protein-blm-mab-hl2771-western-blot-14112025184051.jpg)
![ELISA: Human ATM ELISA Kit (Colorimetric) [NBP2-69891] - Samples were spiked with high concentrations of Human ATM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69891_human-atm-elisa-kit-colorimetric-132202416133650.jpg "ELISA: Human ATM ELISA Kit (Colorimetric) [NBP2-69891] - Samples were spiked with high concentrations of Human ATM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human ATM ELISA Kit (Colorimetric) [NBP2-69891] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69891_human-atm-elisa-kit-colorimetric-432024903543.jpg)
![Immunoprecipitation:[NBP3-30522]-Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of BLM. For blotting immunoprecipitated BLM, was used at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30522_rabbit-blooms-syndrome-protein-blm-pab-2312025129511.jpg "Immunoprecipitation:[NBP3-30522]-Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of BLM. For blotting immunoprecipitated BLM, was used at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334] - Anti-BLMH/Bleomycin Hydrolase rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa](http://images.novusbio.com/fullsize/BLMH-Bleomycin-Hydrolase-Antibody-Western-Blot-NBP2-98334-img0003.jpg "Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334] - Anti-BLMH/Bleomycin Hydrolase rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa")
![Immunoprecipitation: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334] - Mouse BLMH/Bleomycin Hydrolase was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 0.5 ul anti-Mouse BLMH/Bleomycin Hydrolase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse BLMH/Bleomycin Hydrolase rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa](http://images.novusbio.com/fullsize/BLMH-Bleomycin-Hydrolase-Antibody-Immunoprecipitation-NBP2-98334-img0001.jpg)
![Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP2-99780] - Anti-BLMH/Bleomycin Hydrolase rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa](http://images.novusbio.com/fullsize/BLMH-Bleomycin-Hydrolase-Antibody-Western-Blot-NBP2-99780-img0004.jpg "Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP2-99780] - Anti-BLMH/Bleomycin Hydrolase rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa")
![Immunoprecipitation: BLMH/Bleomycin Hydrolase Antibody [NBP2-99780] - BLMH/Bleomycin Hydrolase was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-BLMH/Bleomycin Hydrolase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-BLMH/Bleomycin Hydrolase rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa](http://images.novusbio.com/fullsize/BLMH-Bleomycin-Hydrolase-Antibody-Immunoprecipitation-NBP2-99780-img0003.jpg)
![Western Blot: XRCC3 Antibody (SR1724) [NBP3-22361] - Western blot analysis using NBP3-22361 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22361_rabbit-xrcc3-mab-sr1724-5720231558480.jpg "Western Blot: XRCC3 Antibody (SR1724) [NBP3-22361] - Western blot analysis using NBP3-22361 in HeLa cell lysate.")
