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![Immunocytochemistry/ Immunofluorescence: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Immunocytochemistry analysis of HepG2 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32001_rabbit-adamts5-mab-psh03-52-29202410334088.jpg "Immunocytochemistry/ Immunofluorescence: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Immunocytochemistry analysis of HepG2 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Flow cytometric analysis of HepG2 cells labeling ADAMTS5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32001, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32001_rabbit-adamts5-mab-psh03-52-392024884650.jpg)
![Western Blot: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Western blot analysis of ADAMTS5 on different lysates with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LO2 cell lysate Lane 3: Huh7 cell lysate Lane 4: HeLa cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Human liver tissue lysate Lane 8: Mouse placenta tissue lysate Lane 9: Mouse uterus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 75 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32001) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32001_rabbit-adamts5-mab-psh03-52-3920248453535.jpg)
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Colon, Carcinoma](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0006.jpg "Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Colon, Carcinoma")
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Analysis of anti-ADAMTS5 antibody with cartilage at 3 ug/ ml.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0001.jpg)
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Analysis of anti-ADAMTS5 antibody with human breast, carcinoma.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0002.jpg)
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0003.jpg)
![Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7761] - Anti-ADAMTS5 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/ADAMTS5-Antibody-Immunohistochemistry-Paraffin-NLS7761-img0005.jpg)
![Western Blot: GPR12 Antibody [NLS78] - Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.](http://images.novusbio.com/fullsize/GPR12-Antibody-Western-Blot-NLS78-img0002.jpg "Western Blot: GPR12 Antibody [NLS78] - Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.")
![Immunohistochemistry-Paraffin: GPR12 Antibody [NLS78] - Ovary, Carcinoma embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR12-Antibody-Immunohistochemistry-Paraffin-NLS78-img0003.jpg)
![Immunohistochemistry-Paraffin: GPR12 Antibody [NLS78] - Analysis of anti-GPR12 / GPCR12 antibody with human brain, pituitary at 2 ug/ml.](http://images.novusbio.com/fullsize/GPR12-Antibody-Immunohistochemistry-Paraffin-NLS78-img0001.jpg)
![Western Blot: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Western blot analysis of SIRP alpha/CD172a on different lysates with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/500 dilution. Lane 1: THP-1 cell lysate (10 ug/Lane) Lane 2: HL-60 cell lysate (10 ug/Lane) Lane 3: Mouse brain tissue lysate (20 ug/Lane) Predicted band size: 55 kDa Observed band size: 75-100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32950) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-288202412442941.png "Western Blot: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Western blot analysis of SIRP alpha/CD172a on different lysates with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/500 dilution. Lane 1: THP-1 cell lysate (10 ug/Lane) Lane 2: HL-60 cell lysate (10 ug/Lane) Lane 3: Mouse brain tissue lysate (20 ug/Lane) Predicted band size: 55 kDa Observed band size: 75-100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32950) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32950) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-298202413585563.jpg)
![Flow Cytometry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Flow cytometric analysis of THP-1 cells labeling SIRP alpha/CD172a. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32950, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-2920241637213.png)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1184] - Anti-GPER1 / GPR30 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1184-img0008.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1184] - Anti-GPER1 / GPR30 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1184] - Analysis of anti-GPER1 / GPR30 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1184-img0003.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1184] - Anti-GPER1 / GPR30 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1184-img0006.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1184] - Anti-GPR30 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1184-img0005.jpg)
![Immunohistochemistry-Paraffin: Melatonin R1A/MT1/MTNR1A Antibody [NBP3-14400] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney](http://images.novusbio.com/fullsize/Melatonin-R1A-MT1-MTNR1A-Antibody-Immunohistochemistry-Paraffin-NBP3-14400-img0004.jpg "Immunohistochemistry-Paraffin: Melatonin R1A/MT1/MTNR1A Antibody [NBP3-14400] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney")
![Immunohistochemistry-Paraffin: Melatonin R1A/MT1/MTNR1A Antibody [NBP3-14400] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, hypothalamus, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Melatonin-R1A-MT1-MTNR1A-Antibody-Immunohistochemistry-Paraffin-NBP3-14400-img0001.jpg)
![Immunohistochemistry-Paraffin: Melatonin R1A/MT1/MTNR1A Antibody [NBP3-14400] - Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate](http://images.novusbio.com/fullsize/Melatonin-R1A-MT1-MTNR1A-Antibody-Immunohistochemistry-Paraffin-NBP3-14400-img0002.jpg)
![Immunohistochemistry-Paraffin: Melatonin R1A/MT1/MTNR1A Antibody [NBP3-14400] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, neurons and glia, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Melatonin-R1A-MT1-MTNR1A-Antibody-Immunohistochemistry-Paraffin-NBP3-14400-img0003.jpg)
![Immunohistochemistry: Melatonin R1A/MT1/MTNR1A Antibody [NBP3-14400] - Immunohistochemistry of brain, Globus Pallidus,](http://images.novusbio.com/fullsize/Melatonin R1A-MT1-MTNR1A Antibody-Immunohistochemistry-NBP3-14400-img0005.jpg)
![Western Blot: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Western blot analysis of Glutamate Receptor 4 on different lysates with Rabbit anti-Glutamate Receptor 4 antibody (NBP3-32384) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate (RIPA lysis) Lane 2: Mouse cerebellum tissue lysate (hot lysis) Lane 3: Rat cerebellum tissue lysate (RIPA lysis) Lane 4: Rat cerebellum tissue lysate (hot lysis) Lysates/proteins at 40 ug/Lane. Predicted band size: 101 kDa Observed band size: 110/60 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32384) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32384_rabbit-glutamate-receptor-4-mab-je36-81-278202415242621.jpg "Western Blot: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Western blot analysis of Glutamate Receptor 4 on different lysates with Rabbit anti-Glutamate Receptor 4 antibody (NBP3-32384) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate (RIPA lysis) Lane 2: Mouse cerebellum tissue lysate (hot lysis) Lane 3: Rat cerebellum tissue lysate (RIPA lysis) Lane 4: Rat cerebellum tissue lysate (hot lysis) Lysates/proteins at 40 ug/Lane. Predicted band size: 101 kDa Observed band size: 110/60 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32384) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Flow cytometric analysis of SH-SY5Y cells labeling Glutamate Receptor 4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32384, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32384_rabbit-glutamate-receptor-4-mab-je36-81-392024884649.jpg)
![Immunohistochemistry: NK2R Antibody [NBP3-14421] - Immunohistochemistry of breast carcinoma](http://images.novusbio.com/fullsize/NK2R-Antibody-Immunohistochemistry-NBP3-14421-img0004.jpg "Immunohistochemistry: NK2R Antibody [NBP3-14421] - Immunohistochemistry of breast carcinoma")
![Immunohistochemistry-Paraffin: NK2R Antibody [NBP3-14421] - Immunohistochemistry of formalin-fixed, paraffin-embedded human breast carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NK2R-Antibody-Immunohistochemistry-Paraffin-NBP3-14421-img0001.jpg)
![Immunohistochemistry-Paraffin: NK2R Antibody [NBP3-14421] - Immunohistochemistry of formalin-fixed, paraffin-embedded human small intestine, muscularis propria, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NK2R-Antibody-Immunohistochemistry-Paraffin-NBP3-14421-img0002.jpg)
![Immunohistochemistry-Paraffin: NK2R Antibody [NBP3-14421] - Immunohistochemistry of formalin-fixed, paraffin-embedded human colon after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NK2R-Antibody-Immunohistochemistry-Paraffin-NBP3-14421-img0003.jpg)
![Immunohistochemistry: NK2R Antibody [NBP3-14421] - Immunohistochemistry of ovary carcinoma](http://images.novusbio.com/fullsize/NK2R Antibody-Immunohistochemistry-NBP3-14421-img0005.jpg)
![Immunohistochemistry-Paraffin: GPR84 Antibody [NLS343] - Brain, Alzheimer's disease, senile plaque](http://images.novusbio.com/fullsize/GPR84-Antibody-Immunohistochemistry-Paraffin-NLS343-img0010.jpg "Immunohistochemistry-Paraffin: GPR84 Antibody [NLS343] - Brain, Alzheimer's disease, senile plaque")
![Immunohistochemistry-Paraffin: GPR84 Antibody [NLS343] - Analysis of anti-GPR84 antibody with brain, cell processes.](http://images.novusbio.com/fullsize/GPR84-Antibody-Immunohistochemistry-NLS343-img0006.jpg)
![Immunohistochemistry-Paraffin: GPR84 Antibody [NLS343] - Analysis of anti-GPR84 antibody in human spleen tissue](http://images.novusbio.com/fullsize/GPR84-Antibody-Immunohistochemistry-Paraffin-NLS343-img0007.jpg)
![Immunohistochemistry-Paraffin: GPR84 Antibody [NLS343] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/GPR84-Antibody-Immunohistochemistry-Paraffin-NLS343-img0009.jpg)
![Immunohistochemistry-Paraffin: LPAR6/P2RY5 Antibody [NLS850] - Brain, cortex, neuron](http://images.novusbio.com/fullsize/LPAR6-P2RY5-Antibody-Immunohistochemistry-Paraffin-NLS850-img0006.jpg "Immunohistochemistry-Paraffin: LPAR6/P2RY5 Antibody [NLS850] - Brain, cortex, neuron")
![Immunohistochemistry-Paraffin: LPAR6/P2RY5 Antibody [NLS850] - Analysis of anti-LPAR6 / P2RY5 / P2Y5 antibody with human thymus.](http://images.novusbio.com/fullsize/P2RY5-Antibody-Immunohistochemistry-Paraffin-NLS850-img0002.jpg)
![Immunohistochemistry-Paraffin: LPAR6/P2RY5 Antibody [NLS850] - Analysis of anti-LPAR6 / P2RY5 / P2Y5 antibody with human lung, adenocarcinoma.](http://images.novusbio.com/fullsize/P2RY5-Antibody-Immunohistochemistry-Paraffin-NLS850-img0003.jpg)
![Immunohistochemistry-Paraffin: LPAR6/P2RY5 Antibody [NLS850] - Human Prostate, Carcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/P2RY5-Antibody-Immunohistochemistry-Paraffin-NLS850-img0004.jpg)
![Immunohistochemistry-Paraffin: LPAR6/P2RY5 Antibody [NLS850] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/LPAR6-P2RY5-Antibody-Immunohistochemistry-Paraffin-NLS850-img0005.jpg)
![Immunohistochemistry-Paraffin: GPRC5D Antibody [NLS571] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/GPRC5D-Antibody-Immunohistochemistry-Paraffin-NLS571-img0005.jpg "Immunohistochemistry-Paraffin: GPRC5D Antibody [NLS571] - Brain, Glioblastoma")
![Immunohistochemistry-Paraffin: GPRC5D Antibody [NLS571] - Analysis of anti-GPRC5D antibody with human kidney, renal tubule.](http://images.novusbio.com/fullsize/GPRC5D-Antibody-Immunohistochemistry-Paraffin-NLS571-img0002.jpg)
![Immunohistochemistry-Paraffin: GPRC5D Antibody [NLS571] - Brain, Substantia nigra](http://images.novusbio.com/fullsize/GPRC5D-Antibody-Immunohistochemistry-Paraffin-NLS571-img0003.jpg)
![Immunohistochemistry-Paraffin: GPRC5D Antibody [NLS571] - Brain, Amygdala, Neurons and Glia](http://images.novusbio.com/fullsize/GPRC5D-Antibody-Immunohistochemistry-Paraffin-NLS571-img0004.jpg)
![Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody [NLS387] - Analysis of anti-P2RY1 / P2Y1 antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-Immunohistochemistry-Paraffin-NLS387-img0001.jpg "Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody [NLS387] - Analysis of anti-P2RY1 / P2Y1 antibody with human brain, neurons and glia.")
![Immunohistochemistry: P2Y1/P2RY1 Antibody - BSA Free [NLS387] - Immunohistochemistry of Lung, adenocarcinoma](http://images.novusbio.com/fullsize/nls387_rabbit-p2y1-p2ry1-pab-bsa-free-1092024110311.png)