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![Western Blot: ROBO1 Knockout A549 Cell Lysate [NBP3-18631] - Lane 1: Opal Prestained MW Marker. Lane 2: A549 ROBO KO Clone 5. Lane 3: A549 ROBO KO Clone 15. Lane 4: A549 ROBO KO Clone 17. Lane 5: A549 ROBO KO Clone 18. Lane 6: A549 ROBO KO Clone 21. Lane 7: A549 ROBO1 KO Bulk. Lane 8: A549 WCL Parental. Lane 9: MCF-7 WCL. Load: 35ug lysate/lane. Primary Antibody [Blot A] Anti-ROBO1 ~181kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: TTBS/Casein at RT. No detection of expected band at ~181kDa is observed in ROBO1 knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ROBO1-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18631-img0001.jpg "Western Blot: ROBO1 Knockout A549 Cell Lysate [NBP3-18631] - Lane 1: Opal Prestained MW Marker. Lane 2: A549 ROBO KO Clone 5. Lane 3: A549 ROBO KO Clone 15. Lane 4: A549 ROBO KO Clone 17. Lane 5: A549 ROBO KO Clone 18. Lane 6: A549 ROBO KO Clone 21. Lane 7: A549 ROBO1 KO Bulk. Lane 8: A549 WCL Parental. Lane 9: MCF-7 WCL. Load: 35ug lysate/lane. Primary Antibody [Blot A] Anti-ROBO1 ~181kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: TTBS/Casein at RT. No detection of expected band at ~181kDa is observed in ROBO1 knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Western Blot: Osteocalcin Antibody [NBP3-13133] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Osteocalcin-Antibody-Western-Blot-NBP3-13133-img0002.jpg "Western Blot: Osteocalcin Antibody [NBP3-13133] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Osteocalcin Antibody [NBP3-13133] - Recombinant Osteocalcin were separated by 10% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Osteocalcin-Antibody-Western-Blot-NBP3-13133-img0001.jpg)
![ELISA: Osteocalcin Antibody [NBP3-13133] - Indirect ELISA analysis was performed by coating plate with 50 uL of recombinant full-length Osteocalcin protein at concentrations ranging from 0.938 ng/mL to 60 ng/mL. The coated protein is detected with Osteocalcin antibody (NBP3-13133) at 1 ug/mL.](http://images.novusbio.com/fullsize/Osteocalcin-Antibody-ELISA-NBP3-13133-img0003.jpg)
![Western Blot: Claudin-3 Antibody (HL1528) - Azide and BSA Free [NBP3-25413] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1528] (NBP3-25413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25413_rabbit-claudin-3-mab-hl1528-azide-and-bsa-free-2120249351346.jpg "Western Blot: Claudin-3 Antibody (HL1528) - Azide and BSA Free [NBP3-25413] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1528] (NBP3-25413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1528) - Azide and BSA Free [NBP3-25413] - Claudin 3 antibody [HL1528] detects Claudin 3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1528] (NBP3-25413) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25413_rabbit-claudin-3-mab-hl1528-azide-and-bsa-free-2120249355520.jpg)
![Immunohistochemistry-Paraffin: Involucrin Antibody (rIVRN/827) [NBP3-07559] - Formalin-fixed, paraffin-embedded human Skin stained with Involucrin Mouse Recombinant Monoclonal Antibody (rIVRN/827).](http://images.novusbio.com/fullsize/Involucrin-Antibody-rIVRN-827-Immunohistochemistry-Paraffin-NBP3-07559-img0001.jpg "Immunohistochemistry-Paraffin: Involucrin Antibody (rIVRN/827) [NBP3-07559] - Formalin-fixed, paraffin-embedded human Skin stained with Involucrin Mouse Recombinant Monoclonal Antibody (rIVRN/827).")
![Immunohistochemistry-Paraffin: Involucrin Antibody (IVRN/2113R) [NBP3-07577] - Formalin-fixed, paraffin-embedded human Tonsil stained with Involucrin Rabbit Recombinant Monoclonal Antibody (IVRN/2113R).](http://images.novusbio.com/fullsize/Involucrin-Antibody-IVRN-2113R-Immunohistochemistry-Paraffin-NBP3-07577-img0001.jpg "Immunohistochemistry-Paraffin: Involucrin Antibody (IVRN/2113R) [NBP3-07577] - Formalin-fixed, paraffin-embedded human Tonsil stained with Involucrin Rabbit Recombinant Monoclonal Antibody (IVRN/2113R).")
![Western Blot: CA125/MUC16 Antibody (OTI2C4) [NBP2-46051] - E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 10329-10628 of human MUC16 (NP_078966) were separated by SDS-PAGE and immunoblotted with MUC16.](http://images.novusbio.com/fullsize/CA125-MUC16-Antibody-OTI2C4-Western-Blot-NBP2-46051-img0002.jpg "Western Blot: CA125/MUC16 Antibody (OTI2C4) [NBP2-46051] - E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 10329-10628 of human MUC16 (NP_078966) were separated by SDS-PAGE and immunoblotted with MUC16.")
![Immunohistochemistry: CA125/MUC16 Antibody (OTI2C4) [NBP2-46051] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CA125-MUC16-Antibody-OTI2C4-Immunohistochemistry-NBP2-46051-img0001.jpg)