| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92909-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92877-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37259-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62565-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48408 | |||
| Azide and BSA Free | NBP3-11479 | |||
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Rat lung, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0005.jpg "Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Rat lung, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Mouse heart, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0004.jpg)
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of HepG2 cells, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of NIH/3T3 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025126533.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of Hep G2 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025125497.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of C6 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025125425.jpg)
![Western Blot: ACTL6B Antibody [NBP2-92909] - Analysis of extracts of various cell lines, using ACTL6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Western-Blot-NBP2-92909-img0005.jpg "Western Blot: ACTL6B Antibody [NBP2-92909] - Analysis of extracts of various cell lines, using ACTL6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.")
![Immunocytochemistry/Immunofluorescence: ACTL6B Antibody [NBP2-92909] - Analysis of A-549 cells using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92909-img0001.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded mouse jejunum using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0004.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded human kidney cancer using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0002.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded human kidney using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0003.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Western-Blot-NBP3-15309-img0004.jpg "Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunocytochemistry-Immunofluorescence-NBP3-15309-img0002.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Western-Blot-NBP3-15309-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunofluorescence analysis of RD cells using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunocytochemistry-Immunofluorescence-NBP3-15309-img0001.jpg)
![Immunoprecipitation: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug Collagen III alpha 1/COL3A1 antibody (NBP3-15309). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunoprecipitation-NBP3-15309-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [Collagen III alpha 1/COL3A1] - Confocal imaging of HeLa cells using Collagen III alpha 1/COL3A1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15309_rabbit-collagen-iii-alpha-1-col3a1-mab-6y9c6-52202517273527.jpg)
![Western Blot: CD44 Antibody (9W6E7) [CD44] - Western blot analysis of various lysates using CD44 Rabbit mAb at 1:20000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-5220251734544.jpg "Western Blot: CD44 Antibody (9W6E7) [CD44] - Western blot analysis of various lysates using CD44 Rabbit mAb at 1:20000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 45s.")
![Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD44 Rabbit mAb at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517315598.jpg)
![Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] - Immunohistochemistry analysis of paraffin-embedded Human skin using CD44 Rabbit mAb at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517344236.jpg)
![Immunocytochemistry/ Immunofluorescence: CD44 Antibody (9W6E7) [CD44] - Confocal imaging of HeLa cells using CD44 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517352722.jpg)
![Flow Cytometry: CD44 Antibody (9W6E7) [CD44] - Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and HeLa cells were surface-stained with CD44 Rabbit mAb or ABflo 647 Rabbit IgG isotype control , followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-5220251735270.jpg)
![Flow Cytometry: CD44 Antibody (9W6E7) [CD44] - Flow cytometry: 1X10^6 HeLa cells were surface-stained with ABflo 647 Rabbit IgG isotype control or CD44 Rabbit mAb .](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517304969.jpg)
![Western Blot: Versican Antibody (6D8K9) [NBP3-15841] - Analysis of extracts of various cell lines, using Versican antibody (NBP3-15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Western-Blot-NBP3-15841-img0007.jpg "Western Blot: Versican Antibody (6D8K9) [NBP3-15841] - Analysis of extracts of various cell lines, using Versican antibody (NBP3-15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: Versican Antibody (6D8K9) [NBP3-15841] - Immunofluorescence analysis of NIH-3T3 cells using Versican Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15841_rabbit-versican-mab-6d8k9-72202512443356.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Mouse brain using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0003.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Rat kidney using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0001.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Human esophageal cancer using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0002.jpg)
![Western Blot: Involucrin Antibody (8H7B3) [NBP3-15461] - Analysis of extracts of Rat lung cells, using Involucrin antibody (NBP3-15461) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Involucrin-Antibody-8H7B3-Western-Blot-NBP3-15461-img0004.jpg "Western Blot: Involucrin Antibody (8H7B3) [NBP3-15461] - Analysis of extracts of Rat lung cells, using Involucrin antibody (NBP3-15461) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461] - Mouse skin using Involucrin Rabbit mAb (NBP3-15461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Involucrin-Antibody-8H7B3-Immunohistochemistry-Paraffin-NBP3-15461-img0003.jpg)
![Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461] - Rat skin using Involucrin Rabbit mAb (NBP3-15461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Involucrin-Antibody-8H7B3-Immunohistochemistry-Paraffin-NBP3-15461-img0001.jpg)
![Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461] - Human lung squamous carcinoma tissue using Involucrin Rabbit mAb (NBP3-15461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Involucrin-Antibody-8H7B3-Immunohistochemistry-Paraffin-NBP3-15461-img0002.jpg)
![Immunohistochemistry: Involucrin Antibody (8H7B3) [Involucrin] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Involucrin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15461_rabbit-involucrin-mab-8h7b3-5220251718922.jpg)
![Western Blot: Collagen I alpha 1 Antibody [NBP2-92877] - Analysis of extracts of various cell lines, using Collagen I/COL1A1 antibody at 1:2680 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Western-Blot-NBP2-92877-img0003.jpg "Western Blot: Collagen I alpha 1 Antibody [NBP2-92877] - Analysis of extracts of various cell lines, using Collagen I/COL1A1 antibody at 1:2680 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP2-92877] - Human breast cancer using Collagen I/COL1A1 Rabbit pAb at dilution of 1:10000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-92877-img0005.jpg)
![Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP2-92877] - Human colon using Collagen I/COL1A1 Rabbit pAb at dilution of 1:10000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-92877-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92877] - Immunofluorescence analysis of NIH/3T3 cells using Collagen I alpha 1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92877_rabbit-polyclonal-collagen-i-alpha-1-antibody-422025143183.jpg)
![Western Blot: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Western blot analysis using DSG3 mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-6G2C11-Western-Blot-NBP2-37259-img0004.jpg "Western Blot: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Western blot analysis using DSG3 mouse mAb against A431 cell lysate.")
![Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Analysis of esophageal tissues using DSG3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-6G2C11-Immunohistochemistry-NBP2-37259-img0007.jpg)
![Flow Cytometry: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Flow cytometric analysis of A431 cells using DSG3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-6G2C11-Flow-Cytometry-NBP2-37259-img0005.jpg)
![Western Blot: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Western blot analysis using DSG3 mAb against human DSG3 (AA: 55-159) recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-6G2C11-Western-Blot-NBP2-37259-img0002.jpg)
![Western Blot: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Western blot analysis using DSG3 mAb against HEK293 (1) and DSG3 (AA: 55-159)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-6G2C11-Western-Blot-NBP2-37259-img0003.jpg)
![Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Analysis of esophageal cancer tissues using DSG3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-6G2C11-Immunohistochemistry-NBP2-37259-img0006.jpg)
![ELISA: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-6G2C11-ELISA-NBP2-37259-img0001.jpg)
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435227.jpg "Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/50 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32290, green) at 1/50 dilution overnight at 4 ℃, washed with PBS.<br/><br/> Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with 33258 (blue).](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435238.jpg)
![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202410484863.jpg)
![Flow Cytometry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Flow cytometric analysis of HT-29 (positive, red) and MDA-MB-231 (negative, green) cells labeling E-Cadherin.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32290, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HT-29 black, MDA-MB-231 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202416453231.jpg)
![Western Blot: E-Cadherin Antibody (SY0287) [NBP3-32290] - Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/5,000 dilution.<br/> Lane 1: MCF7 cell lysate<br/> Lane 2: MDA-MB-231 cell lysate (negative)<br/> Lane 3: HT-29 cell lysate<br/> Lane 4: HCT 116 cell lysate<br/> Lane 5: A431 cell lysate<br/> Lane 6: Caco-2 cell lysate<br/><br/> Lysates/proteins at 20 µg/Lane.<br/><br/> Predicted band size: 97 kDa<br/> Observed band size: 80~120 kDa<br/><br/> Exposure time: 43 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-3920248453539.jpg)
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0003.jpg "Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) PE.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0001.jpg)
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) APC.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0002.jpg)
![Western Blot: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Western Blot using anti-MUC1 antibody HMFG2. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of HMFG2 (NBP2-62565) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62565 successfully detected human MUC1 in MCF-7 breast cancer cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Western-Blot-NBP2-62565-img0001.jpg "Western Blot: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Western Blot using anti-MUC1 antibody HMFG2. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of HMFG2 (NBP2-62565) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62565 successfully detected human MUC1 in MCF-7 breast cancer cells.")
![Immunohistochemistry-Paraffin: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Immunohistochemical staining of human lung tissue using anti-MUC1 antibody. HMFG2 Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rabbit-chimeric version of HMFG2 (NBP2-62565). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62565 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong staining of type II pneumocytes may be observed. Recommended concentration, 1-2 ug/ml.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Immunohistochemistry-Paraffin-NBP2-62565-img0003.jpg)
![Flow Cytometry: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Flow-cytometry using the anti-MUC1 antibody HMFG2. MCF-7 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of HMFG2 (NBP2-62565, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Flow-Cytometry-NBP2-62565-img0002.jpg)
![Immunofluorescence: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody HMFG2. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed MCF-7 cells on Shi-fix coverslips stained with the chimeric rabbit version of HMFG2 (NBP2-62565) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62565, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Immunofluorescence-NBP2-62565-img0004.jpg)
![Western Blot: CD44 Antibody (HCAM/918) [NBP2-44652] - DU145 Cell Lysate using CD44 Monoclonal antibody (HCAM/918).](http://images.novusbio.com/fullsize/CD44-Antibody-HCAM-918-Western-Blot-NBP2-44652-img0003.jpg "Western Blot: CD44 Antibody (HCAM/918) [NBP2-44652] - DU145 Cell Lysate using CD44 Monoclonal antibody (HCAM/918).")
![Western Blot: CD44 Antibody (HCAM/918) [NBP2-44652] - Western Blot Analysis of HeLa cell lysate using CD44 Antibody (HCAM/918).](http://images.novusbio.com/fullsize/CD44-Antibody-HCAM-918-Western-Blot-NBP2-44652-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (HCAM/918) [NBP2-44652] - Confocal immunofluorescence image of HeLa cells using CD44 Antibody (HCAM/918) in Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/CD44-Antibody-HCAM-918-Immunocytochemistry-Immunofluorescence-NBP2-44652-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (HCAM/918) [NBP2-44652] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD44 Antibody (HCAM/918).](http://images.novusbio.com/fullsize/CD44-Antibody-HCAM-918-Immunohistochemistry-Paraffin-NBP2-44652-img0005.jpg)