| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89964-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-20694 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50053SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85520-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79835 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12888-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97502-0.2mg | |||
| BSA Free | NBP1-97502SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41319-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55509-25ul | |||
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining in human spleen and skeletal muscle tissues using NBP1-89964 antibody. Corresponding HMOX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining in human spleen and skeletal muscle tissues using NBP1-89964 antibody. Corresponding HMOX1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human spleen shows strong granular cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0021.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human skeletal muscle shows shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0020.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human Fallopian tube shows shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0018.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0019.jpg)
![Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Western-Blot-NBP2-50053-img0002.jpg "Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of of SH-SY5Y cells stained with mouse mAb to calreticulin, NBP2-50053, dilution 1:500 in green and costained with chicken pAb to lamin A/C, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The MCA-6C6 antibody reveals granular staining of cytoplasm, while the lamin AC antibody stains the nuclear lamina and membrane.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Immunocytochemistry-Immunofluorescence-NBP2-50053-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - HeLa cell cultures were stained with NBP2-50053 (red). Calreticulin predominately localized in vesicles and the ER. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, green). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Immunocytochemistry-Immunofluorescence-NBP2-50053-img0003.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0016.jpg "Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85520 (A) shows similar protein distribution across tissues to independent antibody NBP1-85519 (B).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0013.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0014.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0015.jpg)
![Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of human Kidney lysate using Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Western-Blot-NBP2-79701-img0004.jpg "Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of human Kidney lysate using Calnexin Antibody (CANX/1543).")
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1543) [NBP2-79701] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Immunohistochemistry-Paraffin-NBP2-79701-img0001.jpg)
![Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of PANC1 and MCF-7 cell lysate using Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Western-Blot-NBP2-79701-img0003.jpg)
![Protein Array: Calnexin Antibody (CANX/1543) [NBP2-79701] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Calnexin Antibody (CANX/1543). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Protein-Array-NBP2-79701-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Western-Blot-NBP2-12888-img0003.jpg "Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0005.jpg)
![Western Blot: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-FMC-75-Western-Blot-NBP1-97502-img0002.jpg "Western Blot: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.")
![Immunohistochemistry-Paraffin: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of human uterus tissue stained with Calreticulin, mAb (FMC 75) at 10ug/ml.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-FMC-75-Immunohistochemistry-Paraffin-NBP1-97502-img0001.jpg)
![Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-278202415212468.jpg "Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32408) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29820241411540.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunocytochemistry analysis of Hela cells labeling GRP78/HSPA5 with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29202410484873.png)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Immunohistochemistry-Paraffin-NBP3-07740-img0003.jpg "Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).")
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Flow-Cytometry-NBP3-07740-img0001.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Immunohistochemistry-Paraffin-NBP3-07740-img0002.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-NBP2-41319-img0001.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Immunofluorescence of Protein Disulfide Isomerase/P4HB in rat small intestine tissue with Protein Disulfide Isomerase/P4HB antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunofluorescence-NBP2-41319-img0003.jpg)
![Immunohistochemistry: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP2-41319] - Immunohistochemistry of Protein Disulfide Isomerase/P4HB in rat small intestine tissue with Protein Disulfide Isomerase/P4HB antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41319_rabbit-polyclonal-protein-disulfide-isomerase-p4hb-antibody-271120241925150.jpg)
![Western Blot: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Western blot analysis of HSP90 beta on different lysates with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Mouse heart tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-278202415212484.jpg "Western Blot: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Western blot analysis of HSP90 beta on different lysates with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Mouse heart tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32446) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-298202414333651.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunocytochemistry analysis of HeLa cells labeling HSP90 beta with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-2920241048485.jpg)
![Western Blot: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Knockdown-Validated-NBP2-55509-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: HSP90 beta Antibody [NBP2-55509] - Analysis using Anti-HSP90AB1 antibody NBP2-55509 (A) shows similar pattern to independent antibody NBP2-68978 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Western-Blot-NBP2-55509-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP2-55509] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55509-img0001.jpg)