| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86560-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20109-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0080A488-100 | |||
| Alexa Fluor 546 | DDX0080A546-100 | |||
| Alexa Fluor 647 | DDX0080A647-100 | |||
| Biotin | DDX0080B-100 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0020A488-100 | |||
| Alexa Fluor 546 | DDX0020A546-100 | |||
| Biotin | DDX0020B-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44108 | |||
| BSA Free | NBP2-61143-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38684-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91210-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08297 | |||
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder. " alt="CD21 was detected in immersion fixed paraffin-embedded sections of human Lymph Node using Rabbit Anti-Human CD21, Monoclonal Antibody (Catalog # NBP3-20354) at 1:2000 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21126 | |||
![Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Western-Blot-NBP2-76416-img0002.jpg "Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunocytochemistry-NBP2-76416-img0001.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) and rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunoprecipitation-NBP2-76416-img0003.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76416_rabbit-monoclonal-b7-2-cd86-antibody-blr029f-immunoprecipitation-13120251501..jpg)
![Immunohistochemistry-Paraffin: CD1a Antibody [NBP1-86560] - Analysis in human skin and liver tissues using NBP1-86560 antibody. Corresponding CD1A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD1a-Antibody-Immunohistochemistry-Paraffin-NBP1-86560-img0011.jpg "Immunohistochemistry-Paraffin: CD1a Antibody [NBP1-86560] - Analysis in human skin and liver tissues using NBP1-86560 antibody. Corresponding CD1A RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: CD1a Antibody [NBP1-86560] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CD1a-Antibody-Immunohistochemistry-Paraffin-NBP1-86560-img0007.jpg)
![Immunohistochemistry-Paraffin: CD1a Antibody [NBP1-86560] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD1a-Antibody-Immunohistochemistry-Paraffin-NBP1-86560-img0008.jpg)
![Immunohistochemistry-Paraffin: CD1a Antibody [NBP1-86560] - Staining of human skin shows strong granular cytoplasmic positivity in Langerhans cells.](http://images.novusbio.com/fullsize/CD1a-Antibody-Immunohistochemistry-Paraffin-NBP1-86560-img0009.jpg)
![Immunohistochemistry-Paraffin: CD1a Antibody [NBP1-86560] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/CD1a-Antibody-Immunohistochemistry-Paraffin-NBP1-86560-img0010.jpg)
![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded mouse colon tissue using anti-LY75 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0004.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded mouse colon tissue using anti-LY75 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human tonsil tissue using anti-LY75 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0001.jpg)
![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human colon cancer tissue using anti-LY75 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0002.jpg)
![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human kidney tissue using anti-LY75 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0003.jpg)
![Western Blot: CLIP170 Antibody [NBP1-36749] - Analysis of CLIP170 in Human Breast Cancer lysate (35ug protein in RIPA buffer) using this antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Western-Blot-NBP1-36749-img0005.jpg "Western Blot: CLIP170 Antibody [NBP1-36749] - Analysis of CLIP170 in Human Breast Cancer lysate (35ug protein in RIPA buffer) using this antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749] - Staining of paraffin embedded Human Prostate. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Immunohistochemistry-Paraffin-NBP1-36749-img0006.jpg)
![Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749] - Staining of paraffin embedded Human Thyroid Gland. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Immunohistochemistry-Paraffin-NBP1-36749-img0004.jpg)
![Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924288.png "Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-43B-8 (NBP3-20109) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20109, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924248.png)
![Flow Cytometry: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-43B-8 (NBP3-20109, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924262.png)
![Immunohistochemistry: CD1a Antibody (214A9.01) [DDX0080P-100] - Human skin cryosection stained with 214A9](http://images.novusbio.com/fullsize/CD1a-Antibody-214A9-01-Immunohistochemistry-DDX0080P-100-img0010.jpg "Immunohistochemistry: CD1a Antibody (214A9.01) [DDX0080P-100] - Human skin cryosection stained with 214A9")
![Flow Cytometry: CD1a Antibody (214A9.01) [DDX0080P-100] - Facs staining of in vitro-derived DCs with 214A9.01](http://images.novusbio.com/fullsize/CD1a-Antibody-214A9-01-Flow-Cytometry-DDX0080P-100-img0011.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (214A9.01) [DDX0080P-100] - Staining of human lung cancer frozen section.](http://images.novusbio.com/fullsize/CD1a-Antibody-214A9-01-Immunohistochemistry-Frozen-DDX0080P-100-img0005.jpg)
![Immunohistochemistry: DEC-205/CD205 Antibody (NLDC-145) [DDX0020P-100] - Staining of cryosectioned mouse lymph node.](http://images.novusbio.com/fullsize/DEC-205-CD205-Antibody-NLDC-145-Immunohistochemistry-DDX0020P-100-img0001.jpg "Immunohistochemistry: DEC-205/CD205 Antibody (NLDC-145) [DDX0020P-100] - Staining of cryosectioned mouse lymph node.")
![Western Blot: CLIP170 Antibody (CL4844) [NBP2-61143] - analysis in human cell line SiHa.](http://images.novusbio.com/fullsize/CLIP170-Antibody-CL4844-Western-Blot-NBP2-61143-img0001.jpg "Western Blot: CLIP170 Antibody (CL4844) [NBP2-61143] - analysis in human cell line SiHa.")
![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4844) [NBP2-61143] - staining of human placenta shows strong cytoplasmic immunoreactivity in trophoblast.](http://images.novusbio.com/fullsize/CLIP170-Antibody-CL4844-Immunohistochemistry-Paraffin-NBP2-61143-img0005.jpg)
![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4844) [NBP2-61143] - staining of human prostate shows weak cytoplasmic positivity in glandular and smooth muscle cells.](http://images.novusbio.com/fullsize/CLIP170-Antibody-CL4844-Immunohistochemistry-Paraffin-NBP2-61143-img0002.jpg)
![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4844) [NBP2-61143] - staining of human kidney shows moderate cytoplasmic immunoreactivity in a subset of renal tubules.](http://images.novusbio.com/fullsize/CLIP170-Antibody-CL4844-Immunohistochemistry-Paraffin-NBP2-61143-img0003.jpg)
![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4844) [NBP2-61143] - staining of human cervix shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CLIP170-Antibody-CL4844-Immunohistochemistry-Paraffin-NBP2-61143-img0004.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human endometrium, kidney, lymph node and tonsil using Anti-CD21 antibody NBP2-38684 (A) shows similar protein distribution across tissues to independent antibody NBP2-38895 (B).](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0011.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0012.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human kidney shows very weak membranous positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0013.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0014.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38684-img0015.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody [NBP1-91210] - Staining of human testis shows strong membranous and cytoplasmic positivity in spermatids.](http://images.novusbio.com/fullsize/CD11c-Antibody-Immunohistochemistry-Paraffin-NBP1-91210-img0011.jpg "Immunohistochemistry-Paraffin: CD11c Antibody [NBP1-91210] - Staining of human testis shows strong membranous and cytoplasmic positivity in spermatids.")
![Immunohistochemistry-Paraffin: CD11c Antibody [NBP1-91210] - Staining of human lung shows weak membranous and cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CD11c-Antibody-Immunohistochemistry-Paraffin-NBP1-91210-img0008.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody [NBP1-91210] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD11c-Antibody-Immunohistochemistry-Paraffin-NBP1-91210-img0009.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody [NBP1-91210] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD11c-Antibody-Immunohistochemistry-Paraffin-NBP1-91210-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Immunocytochemistry-Immunofluorescence-NBP3-07754-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.")
![Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) [NBP3-07754] - Formalin-fixed, paraffin-embedded human Tonsil Dendritic stained with CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R).](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Immunohistochemistry-Paraffin-NBP3-07754-img0003.jpg)
![Flow Cytometry: CD21 Antibody (CR2/3124R) [NBP3-07754] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Flow-Cytometry-NBP3-07754-img0001.jpg)
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
