| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90058-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50039SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88682-25ul | |||
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A.](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Western-Blot-NBP2-74558-img0001.jpg "Western Blot: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A.")
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0013.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0002.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0003.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0004.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0005.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0006.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0007.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0008.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0009.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0010.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0011.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0012.jpg)
![Immunohistochemistry: TOP2A Antibody (OTI2A9) - Azide and BSA Free [NBP2-74558] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TOP2A-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74558-img0014.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0004.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200).](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0003.jpg)
![Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Wee1-Antibody-Immunohistochemistry-Paraffin-NBP1-33506-img0003.jpg "Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Wee1 Antibody [NBP1-33506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wee1-Antibody-Western-Blot-NBP1-33506-img0007.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-2611202518191318.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251830124.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000 and competitor's antibody (CST#13084) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251829022.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-26112025196217.jpg)
![Western Blot: FGF R1 [p Tyr651, p Tyr652] Antibody [NBP2-24462] - Analysis of FGFR phosphoin recombinant fusion protein containing 1) phosphorylated tyrosine residues at positions 651 and 652 and 2) unphosphorylated tyrosine residues at positions 651 and 652, using FGFR phospho antibody at 0.002 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/FGF-R1-[p-Tyr651--p-Tyr652]-Antibody-Western-Blot-NBP2-24462-img0005.jpg "Western Blot: FGF R1 [p Tyr651, p Tyr652] Antibody [NBP2-24462] - Analysis of FGFR phosphoin recombinant fusion protein containing 1) phosphorylated tyrosine residues at positions 651 and 652 and 2) unphosphorylated tyrosine residues at positions 651 and 652, using FGFR phospho antibody at 0.002 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: FGF R1 [p Tyr651, p Tyr652] Antibody [NBP2-24462] - Analysis of FGFR1 phospho in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control antibody (top left) and FGFR1 phospho antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/FGF-R1-[p-Tyr651--p-Tyr652]-Antibody-Immunohistochemistry-Paraffin-NBP2-24462-img0004.jpg)
![Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-56651-img0005.jpg "Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.")
![Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651] - Analysis of human placenta using PLK1 antibody at 1:100.](http://images.novusbio.com/fullsize/PLK1-Antibody-Immunohistochemistry-Paraffin-NB100-56651-img0004.jpg)
![Western Blot: ZIC3 Antibody [NBP1-33207] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:500](http://images.novusbio.com/fullsize/ZIC3-Antibody-Western-Blot-NBP1-33207-img0001.jpg "Western Blot: ZIC3 Antibody [NBP1-33207] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:500")
![Immunocytochemistry/Immunofluorescence: ZIC3 Antibody [NBP1-33207] - Analysis of paraformaldehyde-fixed Human ESC, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ZIC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33207-img0002.jpg)
![ZIC3 antibody [N3C3] detects ZIC3 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: ZIC3 protein stained by ZIC3 antibody [N3C3] (NBP1-33207) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33207_rabbit-polyclonal-zic3-antibody-immunohistochemistry-frozen-26112025198365.jpg)
![Western Blot: ECHS1 Antibody [NBP2-16264] - ECHS1 antibody detects ECHS1 protein by Western blot analysis. A. 30 ug NT2D1 whole cell lysate/extract B. 30 ug PC-3 whole cell lysate/extractC. 30 ug U87-MG whole cell lysate/extractD. 30 ug SK-N-SH whole cell lysate/extract12 % SDS-PAGEECHS1 antibody d](http://images.novusbio.com/fullsize/ECHS1-Antibody-Western-Blot-NBP2-16264-img0002.jpg "Western Blot: ECHS1 Antibody [NBP2-16264] - ECHS1 antibody detects ECHS1 protein by Western blot analysis. A. 30 ug NT2D1 whole cell lysate/extract B. 30 ug PC-3 whole cell lysate/extractC. 30 ug U87-MG whole cell lysate/extractD. 30 ug SK-N-SH whole cell lysate/extract12 % SDS-PAGEECHS1 antibody d")
![Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16264] - ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ECHS1 protein stained by ECHS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16264-img0005.jpg)
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16264] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16264-img0008.jpg)
![Western Blot: ECHS1 Antibody [NBP2-16264] - ECHS1 antibody detects ECHS1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extractC. 30 ug C8D30 whole cell lysate/extract12 % SDS-PAGEECHS1 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Western-Blot-NBP2-16264-img0001.jpg)
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16264] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16264-img0007.jpg)
![Western Blot: ECHS1 Antibody [NBP2-16264] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody (NBP2-16264) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16264_rabbit-polyclonal-echs1-antibody-91020242001060.jpg)
![Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Wild-type (WT) and Peroxiredoxin 3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Peroxiredoxin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-Western-Blot-NBP2-19777-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Wild-type (WT) and Peroxiredoxin 3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Peroxiredoxin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Peroxiredoxin 3 Antibody [NBP2-19777] - Immunohistochemical analysis of paraffin-embedded Lung AdC, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-19777-img0003.jpg)
![Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 C: Molt-4 D: Raji 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-Western-Blot-NBP2-19777-img0001.jpg)
![Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-Western-Blot-NBP2-19777-img0002.jpg)
![Peroxiredoxin 3 antibody [N1C3] detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [N1C3] (NBP2-19777) diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19777_rabbit-polyclonal-peroxiredoxin-3-antibody-immunocytochemistry-immunofluorescence-261120251840485.jpg)
![Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Western-Blot-NBP2-50039-img0006.jpg "Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - High magnification confocal immunofluorescence of HeLa cells stained with NBP2-50039, diluted 1:1,000 in green and counterstained with antibody to vimentin at 1:1,000 dilution in red. Blue is the DNA stain DAPI revealing the nuclei. The MCA-3F11 reveals aurora B localized in midbodies, midzones of dividing cells and also in the nuclei or some cells.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Immunocytochemistry-Immunofluorescence-NBP2-50039-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - HeLa cell cultures were stained with NBP2-50039 (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Immunocytochemistry-Immunofluorescence-NBP2-50039-img0003.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0001.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human spleen shows moderate to strong cytoplasmic positivity.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0019.jpg "Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human spleen shows moderate to strong cytoplasmic positivity.")
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0015.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0016.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0018.jpg)