| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38641-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92044-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16939-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15611-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35329-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03219-20ul | |||
![Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38641_rabbit-cbx3-p-ser-83-pab-101220241243937.jpg "Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from HeLa cells, using CBX3at 1:1000 dilution or HP1 gamma/CBX3 antibody. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38641_rabbit-cbx3-p-ser-83-pab-1012202411572645.jpg)
![Western Blot: DNA Primase large subunit Antibody [NBP2-92044] - Analysis of extracts of mouse spleen, using DNA Primase large subunit at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/DNA-Primase-large-subunit-Antibody-Western-Blot-NBP2-92044-img0001.jpg "Western Blot: DNA Primase large subunit Antibody [NBP2-92044] - Analysis of extracts of mouse spleen, using DNA Primase large subunit at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Western Blot: DNA Primase large subunit Antibody [NBP2-92044] - Analysis of extracts of various cell lines, using DNA Primase large subunit at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.](http://images.novusbio.com/fullsize/DNA-Primase-large-subunit-Antibody-Western-Blot-NBP2-92044-img0002.jpg)
![Western Blot: YAP1 [p Ser128] Antibody [NBP3-16939] - Western blot analysis of extracts of NIH/3T3 cells, using YAP1 antibody (NBP3-16939) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/YAP1-[p-Ser128]-Antibody-Western-Blot-NBP3-16939-img0001.jpg "Western Blot: YAP1 [p Ser128] Antibody [NBP3-16939] - Western blot analysis of extracts of NIH/3T3 cells, using YAP1 antibody (NBP3-16939) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Western Blot: YAP1 [p Ser128] Antibody - Azide and BSA Free [YAP1] - Western blot analysis of lysates from HeLa cells using YAP1 Rabbit pAb at 1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16939_rabbit-yap1-p-ser-128-pab-52202517352786.jpg)
![Western Blot: WDR57 Antibody [NBP3-15611] - Western blot analysis of extracts of various cell lines, using WDR57 antibody (NBP3-15611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/WDR57-Antibody-Western-Blot-NBP3-15611-img0001.jpg "Western Blot: WDR57 Antibody [NBP3-15611] - Western blot analysis of extracts of various cell lines, using WDR57 antibody (NBP3-15611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: WDR57 Antibody [NBP3-15611] - Western blot analysis of extracts of various cell lines, using WDR57 antibody (NBP3-15611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/WDR57-Antibody-Western-Blot-NBP3-15611-img0002.jpg)
![Western Blot: GARS Antibody [NBP3-35329] - Western blot analysis of various lysates using GARS Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35329_rabbit-gars-pab-1012202411334018.jpg "Western Blot: GARS Antibody [NBP3-35329] - Western blot analysis of various lysates using GARS Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33535_rabbit-ras-gap-mab-6w10g6-10122024140418.jpg "Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of lysates from mouse brain, using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33535_rabbit-ras-gap-mab-6w10g6-10122024140449.jpg)
![Immunohistochemistry-Paraffin: HMGN1/HMG14 Antibody [NBP3-03219] - Immunohistochemistry of paraffin-embedded rat brain using HMGN1/HMG14 antibody (NBP3-03219) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Immunohistochemistry-Paraffin-NBP3-03219-img0001.jpg "Immunohistochemistry-Paraffin: HMGN1/HMG14 Antibody [NBP3-03219] - Immunohistochemistry of paraffin-embedded rat brain using HMGN1/HMG14 antibody (NBP3-03219) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: HMGN1/HMG14 Antibody [NBP3-03219] - Immunohistochemistry of paraffin-embedded mouse testis using HMGN1/HMG14 antibody (NBP3-03219) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HMGN1-HMG14 Antibody-Immunohistochemistry-Paraffin-NBP3-03219-img0002.jpg)
![Western Blot: PARP Antibody (4M9X4) - Cleaved [NBP3-15813] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15813) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PARP-Antibody-4M9X4-Cleaved-Western-Blot-NBP3-15813-img0001.jpg "Western Blot: PARP Antibody (4M9X4) - Cleaved [NBP3-15813] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15813) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: PARP Antibody (4M9X4) - Cleaved [PARP] - Immunoprecipitation analysis of 600 ug extracts of Jurkat cells using 3 ug PARP antibody . Western blot was performed from the immunoprecipitate using PARP antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15813_rabbit-parp-mab-4m9x4-cleaved-52202517184530.jpg)
![Western Blot: MCM7 Antibody (15E1-R) [NBP3-32580] - Western blot analysis of MCM7 on different lysates with Mouse anti-MCM7 antibody (NBP3-32580) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: SW480 cell lysate Lane 6: LNCaP cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32580_mouse-mcm7-mab-15e1-r-278202415212442.jpg "Western Blot: MCM7 Antibody (15E1-R) [NBP3-32580] - Western blot analysis of MCM7 on different lysates with Mouse anti-MCM7 antibody (NBP3-32580) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: SW480 cell lysate Lane 6: LNCaP cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Chk1 Antibody [NBP3-30982] - Western analysis of Chk1 protein in the indicated cell lines. Sheep polyclonal antibody was used at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-30982_sheep-chk1-pab-811202420592127.jpg "Western Blot: Chk1 Antibody [NBP3-30982] - Western analysis of Chk1 protein in the indicated cell lines. Sheep polyclonal antibody was used at 1:500 dilution.")
![Western Blot: CDT2 Antibody (JE58-01) [NBP3-32176] - Western blot analysis of CDT2 on different lysates with Rabbit anti-CDT2 antibody (NBP3-32176) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32176_rabbit-cdt2-mab-je58-01-278202415295521.jpg "Western Blot: CDT2 Antibody (JE58-01) [NBP3-32176] - Western blot analysis of CDT2 on different lysates with Rabbit anti-CDT2 antibody (NBP3-32176) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: CDT2 Antibody (JE58-01) [NBP3-32176] - Flow cytometric analysis of HeLa cells labeling CDT2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32176, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32176_rabbit-cdt2-mab-je58-01-29202416372117.jpg)