| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18578-25ul | |||
| Azide and BSA Free | NBP3-44376 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76751-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03484-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93674-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52436-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37488-0.025ml | |||
![Western Blot: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human cell line U-251 MG and human cell line MCF-7.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Western-Blot-NBP3-18578-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human cell line U-251 MG and human cell line MCF-7.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0008.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human skin and skeletal muscle tissues using NBP3-18578 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0001.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human cervical cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0002.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human breast cancer (metastasis to ovary) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0003.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0005.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0006.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human colon shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0007.jpg)
![Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0006.jpg "Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: BANF1 Antibody [NBP1-76751] - Analysis in Hela cell lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: BANF1 Antibody [NBP1-76751] - Independent Antibody Validation via Protein Expression in Human Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1 [NBP1-76750] (2 ug/ml), BANF1 [NBP1-76751] (2 ug/ml) and beta-actin (1.5 ug/ml). 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0004.jpg)
![Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76751] - Figure 5 Immunofluorescence Validation of BANF1 in Human Kideny TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76751_rabbit-polyclonal-banf1-antibody-2711202420324830.jpg)
![Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Western-Blot-NB100-74526-img0005.jpg "Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human kidney tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0010.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human tonsil tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0014.jpg)
![Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts of various cell lines, using Enolase 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP3-03484-img0006.jpg "Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts of various cell lines, using Enolase 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP3-03484] - Analysis of U-2 OS cells using Enolase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03484-img0002.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP3-03484] - Analysis of 200ug extracts of HeLa cells using Enolase 1 antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03484-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP3-03484] - Analysis of NIH-3T3 cells using Enolase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03484-img0001.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP3-03484] - Paraffin-embedded human colon carcinoma using Enolase 1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03484-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using Enolase 1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Knockout-Validated-NBP3-03484-img0005.jpg)
![Western Blot: WDHD1 Antibody [NBP2-93674] - Analysis of extracts of various cell lines, using WDHD1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP2-93674-img0002.jpg "Western Blot: WDHD1 Antibody [NBP2-93674] - Analysis of extracts of various cell lines, using WDHD1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: WDHD1 Antibody [NBP2-93674] - Analysis of U-2 OS cells using WDHD1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93674-img0001.jpg)
![Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP2-93674] - Human esophageal cancer using WDHD1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-93674-img0003.jpg)
![Western Blot: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-Western-Blot-NBP2-37583-img0002.jpg "Western Blot: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis of Hela cells using XRCC5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-Immunocytochemistry-Immunofluorescence-NBP2-37583-img0003.jpg)
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis of human tonsil tissues (left) and human colon cancer tissues (right) using XRCC5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-Immunohistochemistry-NBP2-37583-img0005.jpg)
![Flow Cytometry: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis of Hela cells using XRCC5 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-Flow-Cytometry-NBP2-37583-img0004.jpg)
![ELISA: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-5C5-ELISA-NBP2-37583-img0001.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0007.jpg "Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.")
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0003.jpg)
![Flow Cytometry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - 4: Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Flow-Cytometry-NBP2-52436-img0001.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA: 105-233)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0005.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against human CCNA2 (AA: 105-233) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0004.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0002.jpg)
![ELISA: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-ELISA-NBP2-52436-img0006.jpg)
![Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Western-Blot-NBP2-37488-img0003.jpg "Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody (1F5) [NBP2-37488] - Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Immunocytochemistry-Immunofluorescence-NBP2-37488-img0004.jpg)
![Immunohistochemistry: eIF4B Antibody (1F5) [NBP2-37488] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Immunohistochemistry-NBP2-37488-img0006.jpg)
![Flow Cytometry: eIF4B Antibody (1F5) [NBP2-37488] - Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Flow-Cytometry-NBP2-37488-img0005.jpg)
![Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Western-Blot-NBP2-37488-img0002.jpg)
![ELISA: eIF4B Antibody (1F5) [NBP2-37488] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-ELISA-NBP2-37488-img0001.jpg)
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0002.jpg "Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with feline leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0004.jpg "Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunofluorescence-NBP2-54621-img0006.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54621-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0003.jpg)
![ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.](http://images.novusbio.com/fullsize/HDAC1-Antibody-ELISA-NBP2-54621-img0002.jpg)
![Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Protein-Array-NBP2-54621-img0005.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16838-img0002.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.")
![Chromatin Immunoprecipitation (ChIP): HIST1H3A Antibody [NBP2-16838] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-HIST1H3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16838-img0009.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 ug of Histone H3 (di-methyl Lys4) antibody 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16838-img0003.jpg)
![Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody (NBP2-16838) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16838_rabbit-polyclonal-hist1h3a-antibody-immunocytochemistry-immunofluorescence-2611202518261313.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0009.jpg "Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-33558] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-33558-img0003.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0004.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0005.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0008.jpg)
![Immunoprecipitation: Bub3 Antibody [NBP1-33558] - Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 ug of BUB3 antibody [N1C1]. Western blot analysis was performed using BUB3 antibody [N1C1]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunoprecipitation-NBP1-33558-img0007.jpg)