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![Multiplex Immunoassay: Histone H3 [Dimethyl Lys14] Antibody (RM165) - Azide and BSA Free [NBP3-25999] - RM165 specifically reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with non-modified Lysine 14 (H3k14), monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25999_rabbit-histone-h3-mab-rm165-azide-and-bsa-free-2462024124591.png "Multiplex Immunoassay: Histone H3 [Dimethyl Lys14] Antibody (RM165) - Azide and BSA Free [NBP3-25999] - RM165 specifically reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with non-modified Lysine 14 (H3k14), monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.")
![Western Blot: Histone H3 [Dimethyl Lys14] Antibody (RM165) - Azide and BSA Free [NBP3-25999] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM165 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 14 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25999_rabbit-histone-h3-mab-rm165-azide-and-bsa-free-22620242311021.png)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody (RM340) - Azide and BSA Free [NBP3-25983] - SNAP-ChIP TM / qPCR using Clone RM340. Antibody (3 ug at 17 ug/mL) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10^6 cells). Specificity (left Y-axis; all bars mean +/- SEM from six independent ChIP experiments; note <15% crossreactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.](http://images.novusbio.com/fullsize/nbp3-25983_rabbit-histone-h3-mab-rm340-azide-and-bsa-free-22620242311085.png "Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody (RM340) - Azide and BSA Free [NBP3-25983] - SNAP-ChIP TM / qPCR using Clone RM340. Antibody (3 ug at 17 ug/mL) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10^6 cells). Specificity (left Y-axis; all bars mean +/- SEM from six independent ChIP experiments; note <15% crossreactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.")
![ELISA: Histone H3 [Trimethyl Lys4] Antibody (RM340) - Azide and BSA Free [NBP3-25983] - ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with Histone H3 Lysine 4 Modified (H3K4)), using Clone RM340 (10 ug/mL).](http://images.novusbio.com/fullsize/nbp3-25983_rabbit-histone-h3-mab-rm340-azide-and-bsa-free-22620242217441.png)
![ELISA: Histone H3 [Trimethyl Lys9] Antibody (RM389) - Azide and BSA Free [NBP3-25992] - ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with Histone H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), using Clone RM389 (0.5 ug/mL).](http://images.novusbio.com/fullsize/nbp3-25992_rabbit-histone-h3-mab-rm389-azide-and-bsa-free-24620241332430.png "ELISA: Histone H3 [Trimethyl Lys9] Antibody (RM389) - Azide and BSA Free [NBP3-25992] - ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with Histone H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), using Clone RM389 (0.5 ug/mL).")
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (RM389) - Azide and BSA Free [NBP3-25992] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM389 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 9 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25992_rabbit-histone-h3-mab-rm389-azide-and-bsa-free-2462024133244.png)
![Multiplex Immunoassay: Histone H3 [ac Lys14] Antibody (RM322) - Azide and BSA Free [NBP3-26006] - RM322 specifically reacts to Histone H3 only when modified at both dimethyl-lysine 9 and acetyl-lysine 14 (K9me2/K14ac). No cross reactivity with non-modified Lysine 9/14, methylated Lysine 9 (K9me1,k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY, or methylated Lysine 14 (K14me2) ONLY, in Histone H3.](http://images.novusbio.com/fullsize/nbp3-26006_rabbit-histone-h3-mab-rm322-azide-and-bsa-free-2462024132639.png "Multiplex Immunoassay: Histone H3 [ac Lys14] Antibody (RM322) - Azide and BSA Free [NBP3-26006] - RM322 specifically reacts to Histone H3 only when modified at both dimethyl-lysine 9 and acetyl-lysine 14 (K9me2/K14ac). No cross reactivity with non-modified Lysine 9/14, methylated Lysine 9 (K9me1,k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY, or methylated Lysine 14 (K14me2) ONLY, in Histone H3.")
![Western Blot: Histone H3 [ac Lys14] Antibody (RM322) - Azide and BSA Free [NBP3-26006] - Western Blot of HeLa cells (1) and recombinant histone H3.3 (2) using RM322 at 0.5 ug/mL.](http://images.novusbio.com/fullsize/nbp3-26006_rabbit-histone-h3-mab-rm322-azide-and-bsa-free-22620242217448.png)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys56] Antibody (RM180) - Azide and BSA Free [NBP3-26023] - RM180 specifically reacts to Histone H3 monomethylated at Lysine 56. No cross reactivity with nonmodified Lysine 56, or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp3-26023_rabbit-histone-h3-mab-rm180-azide-and-bsa-free-24620241332445.png "Multiplex Immunoassay: Histone H3 [Monomethyl Lys56] Antibody (RM180) - Azide and BSA Free [NBP3-26023] - RM180 specifically reacts to Histone H3 monomethylated at Lysine 56. No cross reactivity with nonmodified Lysine 56, or other methylations in histone H3.")
![Western Blot: Histone H3 [Monomethyl Lys56] Antibody (RM180) - Azide and BSA Free [NBP3-26023] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H3.3 (2), using RM180 at 1 ug/mL, showed a band of Histone H3 monomethylated at Lysine 56 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26023_rabbit-histone-h3-mab-rm180-azide-and-bsa-free-226202422174421.png)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys18] Antibody (RM167) - Azide and BSA Free [NBP3-26004] - RM167 specifically reacts to Histone H3 monomethylated at Lysine 18. No cross reactivity with nonmodified Lysine 18 or dimethylated Lysine 18](http://images.novusbio.com/fullsize/nbp3-26004_rabbit-histone-h3-mab-rm167-azide-and-bsa-free-2462024132638.png "Multiplex Immunoassay: Histone H3 [Monomethyl Lys18] Antibody (RM167) - Azide and BSA Free [NBP3-26004] - RM167 specifically reacts to Histone H3 monomethylated at Lysine 18. No cross reactivity with nonmodified Lysine 18 or dimethylated Lysine 18")
![Western Blot: Histone H3 [Monomethyl Lys18] Antibody (RM167) - Azide and BSA Free [NBP3-26004] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using RM167 at 1 ug/mL, showed a band of Histone H3 monomethylated at Lysine 18 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26004_rabbit-histone-h3-mab-rm167-azide-and-bsa-free-24620241332457.png)
