| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25996 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34100-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26015 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26014 | |||
![Western Blot: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61549 at 1 ug/ml, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-RM172-Western-Blot-NBP2-61549-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61549 at 1 ug/ml, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. Observed molecular weight is ~17 kDa")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys27] Antibody (RM172) - Azide and BSA Free [NBP2-61549] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM172 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61549_rabbit-monoclonal-histone-h3-ac-lys27-antibody-rm172-immunocytochemistry-immunofluorescence-189202523381724.png)
![Western Blot: RbBP5 Antibody [NBP2-34100] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-NBP2-34100-img0004.jpg "Western Blot: RbBP5 Antibody [NBP2-34100] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG")
![Immunohistochemistry-Paraffin: RbBP5 Antibody [NBP2-34100] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunohistochemistry-Paraffin-NBP2-34100-img0005.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP2-56317] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP2-56317-img0002.jpg "Western Blot: p62/SQSTM1 Antibody [NBP2-56317] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.")
![Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP2-56317] - Staining of human cell line A549 shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56317-img0001.jpg)
![Western Blot: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant Histone H3.3 (3). using NBP2-61544. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys23-Antibody-RM169-Western-Blot-NBP2-61544-img0004.jpg "Western Blot: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant Histone H3.3 (3). using NBP2-61544. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using NBP2-61544 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys23-Antibody-RM169-Immunocytochemistry-Immunofluorescence-NBP2-61544-img0003.jpg)
![Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) [NBP2-61543] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using NBP2-61543, showed a band of Histone H3 unmodified at Lys4. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Unmodified-Lys4-Antibody-RM186-Western-Blot-NBP2-61543-img0006.jpg "Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) [NBP2-61543] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using NBP2-61543, showed a band of Histone H3 unmodified at Lys4. Observed molecular weight is ~17 kDa.")
![Multiplex Immunoassay: Histone H3 [Unmodified Lys4] Antibody (RM186) [NBP2-61543] - NBP2-61543 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61543_rabbit-monoclonal-histone-h3-unmodified-lys4-antibody-rm186-multiplex-immunoassay-18920252352112.png)
![Western Blot: CBX2 Antibody [NBP2-15735] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CBX2-Antibody-Western-Blot-NBP2-15735-img0001.jpg "Western Blot: CBX2 Antibody [NBP2-15735] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CBX2 Antibody [NBP2-15735] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CBX2-Antibody-Immunohistochemistry-Paraffin-NBP2-15735-img0002.jpg)
![Western Blot: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PHC3-Antibody-Western-Blot-NB100-77312-img0003.jpg "Western Blot: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Western Blot: PHC3 Antibody [NB100-77312] - Detection of Human PHC3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PHC3 was also immunoprecipitated by another rabbit anti-PHC3 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/PHC3-Antibody-Western-Blot-NB100-77312-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for IP at 3 ug per reaction. PHC3 was also immunoprecipitated by rabbit anti-PHC3 antibody NB100-77313. For blotting immunoprecipitated PHC3, NB100-77312 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PHC3-Antibody-NB100-77312-img0004.jpg)
![Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 WDR5 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/WDR5-Antibody-Immunohistochemistry-NBP1-30063-img0004.jpg "Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 WDR5 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: WDR5 Antibody [NBP1-30063] - Detection of human WDR5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody A302-429A (lot A302-429A-2) used for IP at 6 ug per reaction. WDR5 was also immunoprecipitated by rabbit anti-WDR5 antibody A302-430A. For blotting immunoprecipitated WDR5, A302-430A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/WDR5-Antibody-Immunoprecipitation-NBP1-30063-img0006.jpg)
![Western Blot: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500 ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500 ng/mL TSA for 4 hours cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 15 kDa Observed band size: 15/14 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32428) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32428_rabbit-histone-h3-ac-lys23-mab-je46-39-278202415295541.jpg "Western Blot: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500 ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500 ng/mL TSA for 4 hours cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 15 kDa Observed band size: 15/14 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32428) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Immunocytochemistry analysis of HeLa cells treated with or without 500 ng/mL TSA for 4 hours labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32428_rabbit-histone-h3-ac-lys23-mab-je46-39-2920241192342.jpg)
![ELISA: p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/p62-SQSTM1-ELISA-Kit-Colorimetric-ELISA-NBP2-61300-img0001.jpg "ELISA: p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![ELISA: p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/p62-SQSTM1-ELISA-Kit-Colorimetric-ELISA-NBP2-61300-img0002.jpg)