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![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-Immunofluorescence-NBP2-59301-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-Chromatin-Immunoprecipitation-NBP2-59301-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K36me3. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-ELISA-NBP2-59301-img0001.jpg)
![Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32067) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32067_rabbit-baf180-pb1-mab-psh02-34-29820241416183.jpg "Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32067) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling BAF180/PB1 with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32067, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32067_rabbit-baf180-pb1-mab-psh02-34-298202414304111.jpg)
![Western Blot: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Western blot analysis of BAF180/PB1 on different lysates with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/2,000 dilution.Lane 1: 293T cell lysateLane 2: HeLa cell lysateLane 3: SK-MEL-28 cell lysateLane 4: Jurkat cell lysateLane 5: NIH/3T3 cell lysateLane 6: C6 cell lysateLane 7: COS-1 cell lysateLysates/proteins at 15 µg/Lane.Predicted band size: 193 kDaObserved band size: 193 kDaExposure time: 1 minute 2 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32067_rabbit-baf180-pb1-mab-psh02-34-3920248485010.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody (GT261) [NBP3-13553] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (acetyl Lys27) antibody [GT261] (NBP3-13553) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-GT261-Western-Blot-NBP3-13553-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys27] Antibody (GT261) [NBP3-13553] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (acetyl Lys27) antibody [GT261] (NBP3-13553) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Histone H3 [ac Lys27] Antibody (GT261) [NBP3-13553] - Histone H3 (acetyl Lys27) antibody [GT261] detects Histone H3 (acetyl Lys27) protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Histone H3 (acetyl Lys27) antibody [GT261] (NBP3-13553) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-GT261-Immunohistochemistry-Paraffin-NBP3-13553-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys27] Antibody (GT261) [NBP3-13553] - Histone H3 (acetyl Lys27) antibody [GT261] detects Histone H3 (acetyl Lys27) protein at nucleus in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H3 (acetyl Lys27) antibody [GT261] (NBP3-13553) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-GT261-Immunohistochemistry-Paraffin-NBP3-13553-img0002.jpg)
![Western Blot: SMARCC2/BAF170 Antibody [NBP3-12872] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC2 antibody (NBP3-12872) diluted at 1:1000.](http://images.novusbio.com/fullsize/SMARCC2-BAF170-Antibody-Western-Blot-NBP3-12872-img0003.jpg "Western Blot: SMARCC2/BAF170 Antibody [NBP3-12872] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC2 antibody (NBP3-12872) diluted at 1:1000.")
![Immunohistochemistry-Paraffin: SMARCC2/BAF170 Antibody [NBP3-12872] - SMARCC2 antibody detects SMARCC2 protein at nucleolus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SMARCC2 antibody (NBP3-12872) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SMARCC2-BAF170-Antibody-Immunohistochemistry-Paraffin-NBP3-12872-img0002.jpg)
![Immunohistochemistry-Paraffin: SMARCC2/BAF170 Antibody [NBP3-12872] - SMARCC2 antibody detects SMARCC2 protein at nucleolus in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. SMARCC2 antibody (NBP3-12872) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SMARCC2-BAF170-Antibody-Immunohistochemistry-Paraffin-NBP3-12872-img0001.jpg)
![Western Blot: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Methyl-Lys4-Antibody-C-15200150-Western-Blot-NBP2-59284-img0001.jpg "Western Blot: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Methyl-Lys4-Antibody-C-15200150-Immunofluorescence-NBP2-59284-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4me1 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Methyl-Lys4-Antibody-C-15200150-Chromatin-Immunoprecipitation-NBP2-59284-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - Cross reactivity of the antibody directed against H3K4me1. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K4me1. The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Methyl-Lys4-Antibody-C-15200150-ELISA-NBP2-59284-img0002.jpg)
![Western Blot: Histone H3 [ac Lys14] Antibody [NBP2-59275] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K14ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14]-Antibody-Western-Blot-NBP2-59275-img0001.jpg "Western Blot: Histone H3 [ac Lys14] Antibody [NBP2-59275] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K14ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14] Antibody [NBP2-59275] - ChIP assays were performed using human HeLa cells, the antibody against H3K14ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the ACTB and GAPDH promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14]-Antibody-Chromatin-Immunoprecipitation-NBP2-59275-img0004.jpg)
![Dot Blot: Histone H3 [ac Lys14] Antibody [NBP2-59275] - To test the cross reactivity of the antibody against H3K14ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14]-Antibody-Dot-Blot-NBP2-59275-img0002.jpg)
![ELISA: Histone H3 [ac Lys14] Antibody [NBP2-59275] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K14ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:73,200.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14]-Antibody-ELISA-NBP2-59275-img0003.jpg)
![Western Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - Western blot was performed on histone extracts (30 ug) from HeLa cells using the antibody against H3K64me3 . The antibody was diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Western-Blot-NBP2-59227-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - Western blot was performed on histone extracts (30 ug) from HeLa cells using the antibody against H3K64me3 . The antibody was diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - ChIP assays were performed using human K562 cells, the antibody against H3K64me3 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and Sata satellite repeats, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Chromatin-Immunoprecipitation-NBP2-59227-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H3K64me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:5,500.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-ELISA-NBP2-59227-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - To check the specificity of the antibody against H3K64me3 a Dot Blot was performed with peptides containing different modifications of histone H3 and H4 or the unmodified H3K64 sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Dot-Blot-NBP2-59227-img0005.jpg)
![Western Blot: Histone H3 [ac Lys36] Antibody [NBP2-59273] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36ac. The antibody was diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Western-Blot-NBP2-59273-img0001.jpg "Western Blot: Histone H3 [ac Lys36] Antibody [NBP2-59273] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36ac. The antibody was diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys36] Antibody [NBP2-59273] - ChIP assays were performed using human HeLa cells, the antibody against H3K36ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the ACTB promoter and for the GAPDH promoter, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59273-img0004.jpg)
![Dot Blot: Histone H3 [ac Lys36] Antibody [NBP2-59273] - To test the cross reactivity of the antibody against H3K36ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Dot-Blot-NBP2-59273-img0002.jpg)
![ELISA: Histone H3 [ac Lys36] Antibody [NBP2-59273] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:10,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-ELISA-NBP2-59273-img0003.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NBP2-59204-img0001.jpg "Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - ChIP assays were performed using human HeLa cells, the antibody directed against H3K9me1 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 10,000 cells. Respectively 1 and 5 ug of the antibody and 5 ug of IgG (negative IP control) were used per ChIP experiment. QPCR was performed with primers for the GAPDH promoter and for the inactive gene MYOD. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K9me1 is preferably present at silent genes.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NBP2-59204-img0004.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9me1 with peptides containing other modifications and the unmodified sequence of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Dot-Blot-NBP2-59204-img0002.jpg)
![ELISA: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human H3K9me1, crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:68,000.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-ELISA-NBP2-59204-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys56] Antibody [NBP2-59214] - ChIP assays were performed using human HeLa cells, the antibody against H3K56ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH promoter and for the EIF4A2 promoter, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys56]-Antibody-Chromatin-Immunoprecipitation-NBP2-59214-img0002.jpg "Chromatin Immunoprecipitation: Histone H3 [ac Lys56] Antibody [NBP2-59214] - ChIP assays were performed using human HeLa cells, the antibody against H3K56ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH promoter and for the EIF4A2 promoter, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![ELISA: Histone H3 [ac Lys56] Antibody [NBP2-59214] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K56ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:15,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys56]-Antibody-ELISA-NBP2-59214-img0001.jpg)
![Dot Blot: Histone H3 [ac Lys56] Antibody [NBP2-59214] - To test the cross reactivity of the antibody against H3K56ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K56. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys56]-Antibody-Dot-Blot-NBP2-59214-img0004.jpg)
![Western Blot: Histone H3 [Monomethyl Lys14] Antibody [NBP3-15944] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys14]-Antibody-Western-Blot-NBP3-15944-img0003.jpg "Western Blot: Histone H3 [Monomethyl Lys14] Antibody [NBP3-15944] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys14] Antibody [NBP3-15944] - Immunofluorescence analysis of U-2 OS cells using Histone H3 pAb (NBP3-15944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys14]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15944-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys14] Antibody [NBP3-15944] - Immunofluorescence analysis of NIH-3T3 cells using Histone H3 pAb (NBP3-15944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys14]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15944-img0001.jpg)
![Western Blot: AEBP2 Antibody [NBP2-92913] - Analysis of extracts of various cell lines, using AEBP2 .Exposure time: 90s.](http://images.novusbio.com/fullsize/AEBP2-Antibody-Western-Blot-NBP2-92913-img0003.jpg "Western Blot: AEBP2 Antibody [NBP2-92913] - Analysis of extracts of various cell lines, using AEBP2 .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: AEBP2 Antibody [NBP2-92913] - Paraffin-embedded mouse brain using AEBP2 .](http://images.novusbio.com/fullsize/AEBP2-Antibody-Immunohistochemistry-Paraffin-NBP2-92913-img0002.jpg)
![Immunohistochemistry-Paraffin: AEBP2 Antibody [NBP2-92913] - Paraffin-embedded human colon carcinoma using AEBP2 .](http://images.novusbio.com/fullsize/AEBP2-Antibody-Immunohistochemistry-Paraffin-NBP2-92913-img0001.jpg)