| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21060 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24107 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02946-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27754-50ul | |||
![Western Blot: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of extracts of various cell lines, using HMGN1/HMG14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Western-Blot-NBP3-02946-img0004.jpg "Western Blot: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of extracts of various cell lines, using HMGN1/HMG14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit")
![Immunocytochemistry/Immunofluorescence: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of HeLa cells using HMGN1/HMG14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02946-img0001.jpg)
![Western Blot: ATM Antibody (6P6C6) [NBP3-15838] - Western blot analysis of extracts of various cell lines, using ATM antibody (NBP3-15838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ATM-Antibody-6P6C6-Western-Blot-NBP3-15838-img0002.jpg "Western Blot: ATM Antibody (6P6C6) [NBP3-15838] - Western blot analysis of extracts of various cell lines, using ATM antibody (NBP3-15838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: ATM Antibody (6P6C6) [ATM] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using ATM Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15838_rabbit-atm-mab-6p6c6-52202517172899.jpg)
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-278202416285244.jpg "Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MLH1 Antibody (PD01-61) [NBP3-32599] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-298202413585516.jpg)
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.](http://images.novusbio.com/fullsize/PD-L1-Antibody-29E-2A3-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09098-img0002.jpg "Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.")
![Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/PD-L1-Antibody-29E-2A3-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09098-img0001.jpg)
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP1-32023] - Paraffin-embedded lung CA patient tumor, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP1-32023-img0003.jpg "Immunohistochemistry-Paraffin: RPA14 Antibody [NBP1-32023] - Paraffin-embedded lung CA patient tumor, using antibody at 1:100 dilution.")
![Western Blot: RPA14 Antibody [NBP1-32023] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPA14 antibody (NBP1-32023) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32023_rabbit-polyclonal-rpa14-antibody-9102024205694.jpg)
![Western Blot: HDAC10 Antibody (SR3178) [NBP3-27754] - Western blot analysis of HDAC10 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27754_rabbit-hdac10-mab-sr3178-75202410133497.jpg "Western Blot: HDAC10 Antibody (SR3178) [NBP3-27754] - Western blot analysis of HDAC10 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.")
![Immunocytochemistry/ Immunofluorescence: HDAC10 Antibody (SR3178) [NBP3-27754] - Immunofluorescent analysis of Hela cells](http://images.novusbio.com/fullsize/nbp3-27754_rabbit-hdac10-mab-sr3178-75202410133457.jpg)