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![Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-Western-Blot-NBP1-77981-img0003.jpg "Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.")
![N/A: Rad9 [p Ser1260] Antibody [NBP1-77981]](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-N-A-NBP1-77981-img0002.jpg)
![Immunohistochemistry:[NBP3-29226]-Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-SMC1.](http://images.novusbio.com/fullsize/nbp3-29226_rabbit-smc1-pab-2312025128832.jpg "Immunohistochemistry:[NBP3-29226]-Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-SMC1.")
![Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NB100-57077-img0001.jpg "Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Choline kinase alpha Antibody [NBP1-56668] - Sample Type: Human Adult Placenta Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Choline-kinase-alpha-Antibody-Western-Blot-NBP1-56668-img0003.jpg "Western Blot: Choline kinase alpha Antibody [NBP1-56668] - Sample Type: Human Adult Placenta Antibody Dilution: 1.0 ug/ml")
![Western Blot: Choline kinase alpha Antibody [NBP1-56668] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: Human Liver](http://images.novusbio.com/fullsize/Choline-kinase-alpha-Antibody-Western-Blot-NBP1-56668-img0004.jpg)
![Western Blot: PPP2R5C Antibody [NBP2-94154] - Analysis of extracts of various cell lines, using PPP2R5C at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Western-Blot-NBP2-94154-img0004.jpg "Western Blot: PPP2R5C Antibody [NBP2-94154] - Analysis of extracts of various cell lines, using PPP2R5C at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Western Blot: Nbs1 Antibody (4G4R1) [NBP3-16355] - Western blot analysis of extracts of PC-3 cells, using Nbs1 Rabbit mAb (NBP3-16355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Nbs1-Antibody-4G4R1-Western-Blot-NBP3-16355-img0001.jpg "Western Blot: Nbs1 Antibody (4G4R1) [NBP3-16355] - Western blot analysis of extracts of PC-3 cells, using Nbs1 Rabbit mAb (NBP3-16355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: Nbs1 Antibody (4G4R1) [NBP3-16355] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Nbs1 antibody (NBP3-16355). Western blot was performed from the immunoprecipitate using Nbs1 antibody (NBP3-16355) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Nbs1-Antibody-4G4R1-Immunoprecipitation-NBP3-16355-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F6A9-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12825-img0001.jpg "Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.")
![Flow Cytometry: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Flow cytometry analysis of PD-L1 transfected 293 cells using PD-L1 [F6A9] antibody at 10 ug/ml. Blue: untransfected cells, Yellow: PD-L1 transfected cells.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F6A9-VHH-Flow-Cytometry-NBP3-12825-img0002.jpg)
![Western Blot: Transportin 1 Antibody [NBP2-93233] - Analysis of extracts of various cell lines, using Transportin 1 . Exposure time: 1s.](http://images.novusbio.com/fullsize/Transportin-1-Antibody-Western-Blot-NBP2-93233-img0001.jpg "Western Blot: Transportin 1 Antibody [NBP2-93233] - Analysis of extracts of various cell lines, using Transportin 1 . Exposure time: 1s.")
![Western Blot: Transportin 1 Antibody [NBP2-94765] - Analysis of extracts of various cell lines, using Transportin 1 . Exposure time: 5s.](http://images.novusbio.com/fullsize/Transportin-1-Antibody-Western-Blot-NBP2-94765-img0001.jpg "Western Blot: Transportin 1 Antibody [NBP2-94765] - Analysis of extracts of various cell lines, using Transportin 1 . Exposure time: 5s.")