| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21060 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14062 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56234-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14124 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15566-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26030 | |||
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Immunocytochemistry-Immunofluorescence-NBP3-13936-img0002.jpg "Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).")
![Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC1 antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Flow-Cytometry-NBP3-13936-img0001.jpg)
![Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC1 Antibody (PCRP-HDAC1-1B7).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Protein-Array-NBP3-13936-img0004.jpg)
![Western Blot: p53 Antibody [NBP2-56234] - Analysis in human cell line U-251 MG and human cell line PC-3.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-56234-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-56234] - Analysis in human cell line U-251 MG and human cell line PC-3.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-56234] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56234-img0001.jpg)
![Western Blot: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - Western blot analysis of Jurkat cell lysate using MLH1 antibody (MLH1/6284R).](http://images.novusbio.com/fullsize/MLH1-Antibody-MLH1-6284R-Western-Blot-NBP3-13971-img0001.jpg "Western Blot: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - Western blot analysis of Jurkat cell lysate using MLH1 antibody (MLH1/6284R).")
![Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MLH1 antibody (MLH1/6284R).](http://images.novusbio.com/fullsize/MLH1-Antibody-MLH1-6284R-Immunohistochemistry-Paraffin-NBP3-13971-img0003.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - MLH1 antibody (MLH1/6284R). MLH1 Recombinant Rabbit Monoclonal (MLH1/6284R). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/MLH1-Antibody-MLH1-6284R-Immunohistochemistry-Paraffin-NBP3-13971-img0002.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - FFPE human Hereditary Nonpolyposis Colorectal Cancer (HNPCC) / Lynch Syndrome. MLH1 antibody (MLH1/6284R) (2ug/ml).](http://images.novusbio.com/fullsize/MLH1-Antibody-MLH1-6284R-Immunohistochemistry-Paraffin-NBP3-13971-img0004.jpg)
![Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Western-Blot-NBP3-15566-img0002.jpg "Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Chromatin Immunoprecipitation: HDAC1 Antibody (2W4I5) [NBP3-15566] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody (NBP3-15566) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Chromatin-Immunoprecipitation-NBP3-15566-img0003.jpg)
![Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.](http://images.novusbio.com/fullsize/PLK1-[p-Ser482--p-Ser486--p-Ser490]-Antibody-Western-Blot-NB300-278-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.")
![Western Blot: PP2A alpha Antibody [NB100-2260] - Detection of Human and Mouse PP2A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NB100-2260-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: PP2A alpha Antibody [NB100-2260] - Detection of Human and Mouse PP2A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: PP2A alpha Antibody [NB100-2260] - Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 (lot 2) used for IP at 6 ug per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-PP2A antibody NB100-2259. For blotting immunoprecipitated PP2A, NB100-2260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunoprecipitation-NB100-2260-img0004.jpg)
![Western Blot: Histone H2AX [p Ser 119] Antibody (RM224) [NBP3-18533] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using NBP3-18533 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H2AX-p-Ser-119-Antibody-RM224-Western-Blot-NBP3-18533-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H2AX [p Ser 119] Antibody (RM224) [NBP3-18533] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using NBP3-18533 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-18533] - Immunocytochemistry of HeLa cells using Clone RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-18533_rabbit-histone-h2ax-p-ser-139-mab-rm224-immunocytochemistry-immunofluorescence-189202523444711.png)
![Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Ser2056]-Antibody-Western-Blot-NBP3-13142-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - DNA-PKcs (phospho Ser2056) antibody detects DNA-PKcs (phospho Ser2056) protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA-PKcs (phospho Ser2056) stained by DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Ser2056]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13142-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Ser2056]-Antibody-Western-Blot-NBP3-13142-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody (HL1210) [NBP3-13663] - ATRX antibody [HL1210] detects ATRX protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATRX stained by ATRX antibody [HL1210] (NBP3-13663) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/ATRX-Antibody-HL1210-Immunocytochemistry-Immunofluorescence-NBP3-13663-img0002.jpg "Immunocytochemistry/Immunofluorescence: ATRX Antibody (HL1210) [NBP3-13663] - ATRX antibody [HL1210] detects ATRX protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATRX stained by ATRX antibody [HL1210] (NBP3-13663) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ATRX Antibody (HL1210) [NBP3-13663] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL1210] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13663_rabbit-atrx-mab-hl1210-1610202419405761.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATRX Antibody (HL1210) [NBP3-13663] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL1210] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13663_rabbit-atrx-mab-hl1210-1610202419405792.jpg)
![Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Chk2 [p Thr68] Antibody [NBP3-13076] - Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chk2 (phospho Thr68) stained by Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13076-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Chk2 [p Thr68] Antibody [NBP3-13076] - Wild-type (WT) and Chk2 (phospho Thr68) knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0003.jpg)