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![Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-27220248471916.jpg "Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.")
![Flow Cytometry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Overlay Peak curve showing SH-SY5Y cells stained with Tau [p Ser199] Antibody (23E8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-282202414183638.jpg)
![Western Blot: Tau Antibody (S03-9I2) [NBP3-20059] - Western blot detection of Tau in Human Hippocampus lysates using NBP3-20059 (1:1000 diluted). Predicted band size: 79KDa. Observed band size: 50-80KDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S03-9I2-Western-Blot-NBP3-20059-img0001.jpg "Western Blot: Tau Antibody (S03-9I2) [NBP3-20059] - Western blot detection of Tau in Human Hippocampus lysates using NBP3-20059 (1:1000 diluted). Predicted band size: 79KDa. Observed band size: 50-80KDa.")
![Western Blot: HSP90 alpha Antibody (S06-9C1) [NBP3-19514] - Western blot detection of HSP90 alpha in K562, C6, Hela cell lysates using NBP3-19514 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-S06-9C1-Western-Blot-NBP3-19514-img0001.jpg "Western Blot: HSP90 alpha Antibody (S06-9C1) [NBP3-19514] - Western blot detection of HSP90 alpha in K562, C6, Hela cell lysates using NBP3-19514 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 90kDa.")
![Western Blot: Tau Antibody (S09-5E2) [NBP3-20020] - Western blot detection of Tau in Rat Brain cell using NBP3-20020 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S09-5E2-Western-Blot-NBP3-20020-img0001.jpg "Western Blot: Tau Antibody (S09-5E2) [NBP3-20020] - Western blot detection of Tau in Rat Brain cell using NBP3-20020 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: Tau [p Ser202] Antibody (S01-5A7) [NBP3-20058] - Western blot detection of Tau in Rat Brain lysates using NBP3-20058 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-p-Ser202-Antibody-S01-5A7-Western-Blot-NBP3-20058-img0001.jpg "Western Blot: Tau [p Ser202] Antibody (S01-5A7) [NBP3-20058] - Western blot detection of Tau in Rat Brain lysates using NBP3-20058 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: Tau [p Ser199] Antibody (JE66-80) [NBP3-32802] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32802) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32802_rabbit-tau-p-ser199-mab-je66-80-288202412442962.jpg "Western Blot: Tau [p Ser199] Antibody (JE66-80) [NBP3-32802] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32802) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32773_rabbit-ikb-alpha-p-ser36-mab-je56-65-288202412442912.jpg "Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser202] Antibody (JE66-81) [NBP3-32804] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32804) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32804) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32804_rabbit-tau-p-ser202-mab-je66-81-288202412442917.jpg "Western Blot: Tau [p Ser202] Antibody (JE66-81) [NBP3-32804] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32804) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32804) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser198] Antibody (JE66-79) [NBP3-32801] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32801) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32801) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32801_rabbit-tau-p-ser198-mab-je66-79-288202412442947.jpg "Western Blot: Tau [p Ser198] Antibody (JE66-79) [NBP3-32801] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32801) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32801) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser356] Antibody (JE46-86) [NBP3-32807] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32807) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32807_rabbit-tau-p-ser356-mab-je46-86-28820241246167.jpg "Western Blot: Tau [p Ser356] Antibody (JE46-86) [NBP3-32807] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32807) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")