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![Western Blot: IkB-alpha Antibody (116) [NBP2-90043] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:38 kDa](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-116-Western-Blot-NBP2-90043-img0001.jpg "Western Blot: IkB-alpha Antibody (116) [NBP2-90043] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:38 kDa")
![Immunoprecipitation: IkB-alpha Antibody (116) [NBP2-90043] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg Hela Whole Cell LysateLane E:0.5 mg K562 Whole Cell Lysate2 uL anti-NFKBIA rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NFKBIA rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-116-Immunoprecipitation-NBP2-90043-img0002.jpg)
![Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/Tau-[p-Ser202-p-Ser199-p-Ser198]-Antibody-Western-Blot-NBP3-16924-img0001.jpg "Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.")
![Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-27220248471916.jpg "Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.")
![Flow Cytometry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Overlay Peak curve showing SH-SY5Y cells stained with Tau [p Ser199] Antibody (23E8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-282202414183638.jpg)
![Western Blot: Tau Antibody (S09-5E2) [NBP3-20020] - Western blot detection of Tau in Rat Brain cell using NBP3-20020 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S09-5E2-Western-Blot-NBP3-20020-img0001.jpg "Western Blot: Tau Antibody (S09-5E2) [NBP3-20020] - Western blot detection of Tau in Rat Brain cell using NBP3-20020 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: Tau Antibody (S03-9I2) [NBP3-20059] - Western blot detection of Tau in Human Hippocampus lysates using NBP3-20059 (1:1000 diluted). Predicted band size: 79KDa. Observed band size: 50-80KDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S03-9I2-Western-Blot-NBP3-20059-img0001.jpg "Western Blot: Tau Antibody (S03-9I2) [NBP3-20059] - Western blot detection of Tau in Human Hippocampus lysates using NBP3-20059 (1:1000 diluted). Predicted band size: 79KDa. Observed band size: 50-80KDa.")
![Western Blot: Tau [p Ser202] Antibody (S01-5A7) [NBP3-20058] - Western blot detection of Tau in Rat Brain lysates using NBP3-20058 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-p-Ser202-Antibody-S01-5A7-Western-Blot-NBP3-20058-img0001.jpg "Western Blot: Tau [p Ser202] Antibody (S01-5A7) [NBP3-20058] - Western blot detection of Tau in Rat Brain lysates using NBP3-20058 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: HSP90 alpha Antibody (S06-9C1) [NBP3-19514] - Western blot detection of HSP90 alpha in K562, C6, Hela cell lysates using NBP3-19514 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-S06-9C1-Western-Blot-NBP3-19514-img0001.jpg "Western Blot: HSP90 alpha Antibody (S06-9C1) [NBP3-19514] - Western blot detection of HSP90 alpha in K562, C6, Hela cell lysates using NBP3-19514 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 90kDa.")
![Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32773_rabbit-ikb-alpha-p-ser36-mab-je56-65-288202412442912.jpg "Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser214] Antibody (JE66-83) [NBP3-32805] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32805) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32805_rabbit-tau-p-ser214-mab-je66-83-288202412442910.jpg "Western Blot: Tau [p Ser214] Antibody (JE66-83) [NBP3-32805] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32805) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")