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![Recombinant Human HSP70/HSPA1A Protein [NBC1-18367]](http://images.novusbio.com/fullsize/Recombinant-Human-HSP70-HSPA1A-Protein-NBC1-18367-img0001.jpg "Recombinant Human HSP70/HSPA1A Protein [NBC1-18367]")
![Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/Tau-[p-Ser202-p-Ser199-p-Ser198]-Antibody-Western-Blot-NBP3-16924-img0001.jpg "Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.")
![Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32773_rabbit-ikb-alpha-p-ser36-mab-je56-65-288202412442912.jpg "Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: IkB-alpha Antibody (116) [NBP2-90043] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:38 kDa](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-116-Western-Blot-NBP2-90043-img0001.jpg "Western Blot: IkB-alpha Antibody (116) [NBP2-90043] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:38 kDa")
![Immunoprecipitation: IkB-alpha Antibody (116) [NBP2-90043] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg Hela Whole Cell LysateLane E:0.5 mg K562 Whole Cell Lysate2 uL anti-NFKBIA rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NFKBIA rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-116-Immunoprecipitation-NBP2-90043-img0002.jpg)
![Western Blot: Tau [p Ser202] Antibody (JE66-81) [NBP3-32804] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32804) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32804) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32804_rabbit-tau-p-ser202-mab-je66-81-288202412442917.jpg "Western Blot: Tau [p Ser202] Antibody (JE66-81) [NBP3-32804] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32804) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32804) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser214] Antibody (JE66-83) [NBP3-32805] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32805) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32805_rabbit-tau-p-ser214-mab-je66-83-288202412442910.jpg "Western Blot: Tau [p Ser214] Antibody (JE66-83) [NBP3-32805] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32805) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser356] Antibody (JE46-86) [NBP3-32807] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32807) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32807_rabbit-tau-p-ser356-mab-je46-86-28820241246167.jpg "Western Blot: Tau [p Ser356] Antibody (JE46-86) [NBP3-32807] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32807) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser199] Antibody (JE46-95) [NBP3-32803] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with pp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32803_rabbit-tau-p-ser199-mab-je46-95-288202412461637.jpg "Western Blot: Tau [p Ser199] Antibody (JE46-95) [NBP3-32803] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with pp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser199] Antibody (JE66-80) [NBP3-32802] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32802) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32802_rabbit-tau-p-ser199-mab-je66-80-288202412442962.jpg "Western Blot: Tau [p Ser199] Antibody (JE66-80) [NBP3-32802] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32802) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser324] Antibody (JE30-73) [NBP3-32806] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32806) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-130 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32806) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32806_rabbit-tau-p-ser324-mab-je30-73-288202412461657.jpg "Western Blot: Tau [p Ser324] Antibody (JE30-73) [NBP3-32806] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32806) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-130 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32806) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser198] Antibody (JE66-79) [NBP3-32801] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32801) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32801) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32801_rabbit-tau-p-ser198-mab-je66-79-288202412442947.jpg "Western Blot: Tau [p Ser198] Antibody (JE66-79) [NBP3-32801] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32801) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32801) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).](http://images.novusbio.com/fullsize/nbp3-23389_rabbit-ikb-alpha-p-tyr42-pab-19920231856943.jpg "Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).")