| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59692-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37525-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1900-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37713 | |||
![Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Western-Blot-NBP2-59692-img0002.jpg "Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Mouse fibroblast cell line (NIH 3T3). Fixed in 4% Formaldehyde for 15 min at RT. Primary antibody at 1:100 for 60 min at RT. Secondary antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstaied with DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Immunocytochemistry-Immunofluorescence-NBP2-59692-img0003.jpg)
![Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Western-Blot-NBP2-37525-img0002.jpg "Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37525-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of brain tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0006.jpg)
![Flow Cytometry: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Flow-Cytometry-NBP2-37525-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of lung cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0005.jpg)
![ELISA: NF-L Antibody (2G10) [NBP2-37525] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-ELISA-NBP2-37525-img0001.jpg)
![Western Blot: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIT ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIT (1:2000). Positive lysates.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Western-Blot-NBP2-71076-img0001.jpg "Western Blot: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIT ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIT (1:2000). Positive lysates.")
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-71076-img0013.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Human colon tissue within the normal limits using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-71076-img0002.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Adenocarcinoma of Human colon tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-71076-img0003.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-71076-img0004.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Carcinoma of Human thyroid tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-71076-img0005.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Human lymphoma tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-71076-img0006.jpg)
![Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human bladder tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71076-img0007.jpg)
![Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71076-img0008.jpg)
![Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71076-img0009.jpg)
![Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71076-img0010.jpg)
![Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71076-img0011.jpg)
![Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71076-img0012.jpg)
![Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control](http://images.novusbio.com/fullsize/Cofilin-Antibody-Western-Blot-NB100-1900-img0002.jpg "Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody [NB100-1900] - NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 1:1,000 Anti-Cofilin antibody produced in Rabbit . The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Cofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1900-img0001.jpg)
![Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32734 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-26820241582612.jpg "Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32734 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL")
![Western Blot: PELP1 Antibody (JE02-42) [NBP3-32734] - Western blot analysis of PELP1 on different lysates with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 120 kDa Observed band size: 150 kDa Exposure time: Lane 1-4: 10 seconds; Lane 5: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-27820241521240.jpg)
![Immunohistochemistry: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32734) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-298202414333664.jpg)
![Immunocytochemistry/ Immunofluorescence: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunocytochemistry analysis of MCF7 cells labeling PELP1 with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-29202410334017.jpg)
![Flow Cytometry: PELP1 Antibody (JE02-42) [NBP3-32734] - Flow cytometric analysis of MCF7 cells labeling PELP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32734, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-29202416453251.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Analysis using the Alexa Fluor (R) 647 conjugate of NB500-523. Staining of CD16a in human buffy coat using anti-CD16a antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Ima](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-MEM-168-Flow-Cytometry-NB500-523-img0005.jpg "Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Analysis using the Alexa Fluor (R) 647 conjugate of NB500-523. Staining of CD16a in human buffy coat using anti-CD16a antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Ima")
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Surface staining of lysed and washed porcine peripheral blood with purified anti-CD16 (MEM-168) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03) Panel B, C, D - three different porcine PMBC samples stained with anti-CD16 (MEM-168) Cells in the granulocyte gate were used for analysis.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-MEM-168-Flow-Cytometry-NB500-523-img0001.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Analysis using the Alexa Fluor (R) 488 conjugate of NB500-523. Staining of CD16a in human buffy coat using anti-CD16a antibody AF488. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-MEM-168-Flow-Cytometry-NB500-523-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Human PBMC stained with Fc gamma RIIIA/CD16a antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-MEM-168-Flow-Cytometry-NB500-523-img0007.jpg)
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0003.jpg "Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) PE.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0001.jpg)
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) APC.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0002.jpg)
![Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Western-Blot-NBP2-75531-img0006.jpg "Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0005.jpg)
![Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Flow-Cytometry-NBP2-75531-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0002.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0003.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0004.jpg)
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/Fc-gamma-RIII-CD16-Antibody-MEM-154-Flow-Cytometry-NB500-378-img0002.jpg "Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Flow cytometry surface staining pattern of human peripheral blood stained using Fc gamma RIII (CD16) Antibody (MEM-154) purified antibody (concentration in sample 2 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIII-CD16-Antibody-MEM-154-Flow-Cytometry-NB500-378-img0001.jpg)
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - BSA Free [NB500-378] - Staining of human peripheral blood cells with anti-human CD16 (MEM-154) purified, GAM/APC.](http://images.novusbio.com/fullsize/nb500-378_mouse-monoclonal-fc-gamma-riii-cd16-antibody-mem-154-17720241003218.jpg)
![Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-288202414191539.jpg "Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33093) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-29820241433367.jpg)
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33093, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-298202414304144.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-2920241114843.jpg)
![Flow Cytometry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33093, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-392024884612.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)
![Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Western-Blot-NBP2-67681-img0003.jpg "Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0006.jpg)
![Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Western-Blot-NBP2-67681-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (red) at 1/200 dilution and Vimentin (green) at 1/200 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0005.jpg)