| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61888-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52448-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52447-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-10535-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-94155-0.025mg | |||
![Western Blot: Apc10 Antibody (3E9A9) [NBP2-61888] - Analysis using ANAPC10 mAb against HEK293 (1) and ANAPC10 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Apc10-Antibody-3E9A9-Western-Blot-NBP2-61888-img0006.jpg "Western Blot: Apc10 Antibody (3E9A9) [NBP2-61888] - Analysis using ANAPC10 mAb against HEK293 (1) and ANAPC10 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Apc10 Antibody (3E9A9) [NBP2-61888] - Analysis of Hela cells using ANAPC10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Apc10-Antibody-3E9A9-Immunocytochemistry-Immunofluorescence-NBP2-61888-img0003.jpg)
![Immunohistochemistry: Apc10 Antibody (3E9A9) [NBP2-61888] - Analysis of paraffin-embedded esophageal cancer tissues using ANAPC10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Apc10-Antibody-3E9A9-Immunohistochemistry-NBP2-61888-img0004.jpg)
![Western Blot: Apc10 Antibody (3E9A9) [NBP2-61888] - Analysis using ANAPC10 mAb against human ANAPC10 (AA: 1-185) recombinant protein. (Expected MW is 47.2 kDa)](http://images.novusbio.com/fullsize/Apc10-Antibody-3E9A9-Western-Blot-NBP2-61888-img0005.jpg)
![Western Blot: Apc10 Antibody (3E9A9) [NBP2-61888] - Analysis using ANAPC10 mouse mAb against Jurkat (1), MCF-7 (2), SK-Br-3 (3), A431 (4), HEK293 (5), A549 (6), and SPC-A-1 (7) cell lysate.](http://images.novusbio.com/fullsize/Apc10-Antibody-3E9A9-Western-Blot-NBP2-61888-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Apc10 Antibody (3E9A9) [NBP2-61888] - Analysis of Hela cells using ANAPC10 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Apc10-Antibody-3E9A9-Immunocytochemistry-Immunofluorescence-NBP2-61888-img0002.jpg)
![ELISA: Apc10 Antibody (3E9A9) [NBP2-61888] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Apc10-Antibody-3E9A9-ELISA-NBP2-61888-img0001.jpg)
![Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Western-Blot-NBP2-52448-img0004.jpg "Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)")
![Immunocytochemistry/Immunofluorescence: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Immunofluorescence-NBP2-52448-img0003.jpg)
![Flow Cytometry: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of A549 cells using CSF3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Flow-Cytometry-NBP2-52448-img0001.jpg)
![Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Western-Blot-NBP2-52448-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Immunofluorescence-NBP2-52448-img0002.jpg)
![ELISA: G-CSF Antibody (8G5F7) [NBP2-52448] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-ELISA-NBP2-52448-img0006.jpg)
![Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Western-Blot-NBP2-52447-img0004.jpg "Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)")
![Immunocytochemistry/Immunofluorescence: G-CSF Antibody (7E4F7) [NBP2-52447] - Immunofluorescence analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Immunofluorescence-NBP2-52447-img0001.jpg)
![Immunohistochemistry: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis of paraffin-embedded esophageal cancer tissues using CSF3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Immunohistochemistry-NBP2-52447-img0003.jpg)
![Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Western-Blot-NBP2-52447-img0005.jpg)
![Immunohistochemistry: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Immunohistochemistry-NBP2-52447-img0002.jpg)
![ELISA: G-CSF Antibody (7E4F7) [NBP2-52447] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-ELISA-NBP2-52447-img0006.jpg)
![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (12B6) [NB110-94155] - Flt-3 antibody staining in mouse lung cancer tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-12B6-Immunohistochemistry-Paraffin-NB110-94155-img0003.jpg "Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (12B6) [NB110-94155] - Flt-3 antibody staining in mouse lung cancer tissue. IHC-P image submitted by a verified customer review.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Flt3/CD135 Antibody (12B6) [NB110-94155] - Paraffin kidney and lung sections from LPS treated mice were subjected to IHC using anti Flt3/CD135 Antibody (12B6) RM0021-12B6](http://images.novusbio.com/fullsize/Flt3-CD135-Antibody-12B6-Immunohistochemistry-NB110-94155-img0002.jpg)
![Immunohistochemistry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32236) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-298202414304156.jpg "Immunohistochemistry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32236) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunocytochemistry analysis of Neuro-2a cells labeling Cyclin D1 with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cyclin D1 antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-29202410484833.jpg)
![Flow Cytometry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Flow cytometric analysis of MCF7 cells labeling Cyclin D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32236, red) at 1/5,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-29202416453255.jpg)
![Western Blot: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Western blot analysis of Cyclin D1 on different lysates with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/5,000 dilution.Lane 1: MCF7 cell lysateLane 2: K-562 cell lysate (negative)Lane 3: A431 cell lysateLane 4: Neuro-2a cell lysateLane 5: NIH/3T3 cell lysateLane 6: C6 cell lysateLane 7: SH-SY5Y cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 34 kDaObserved band size: 35 kDaExposure time: 20 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-3920248485020.jpg)
![Western Blot: KLF11 Antibody [NBP3-10447] - Western blot analysis of KLF11 in Mouse Brain as a positive control. Antibody dilution at 0.2-1 ug/ml](http://images.novusbio.com/fullsize/KLF11-Antibody-Western-Blot-NBP3-10447-img0001.jpg "Western Blot: KLF11 Antibody [NBP3-10447] - Western blot analysis of KLF11 in Mouse Brain as a positive control. Antibody dilution at 0.2-1 ug/ml")
![Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JWA-Antibody-Western-Blot-NBP3-12158-img0002.jpg "Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: JWA Antibody [NBP3-12158] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/JWA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12158-img0003.jpg)
![Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Small Intestine with antibody at 3.75 g/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/JWA-Antibody-Immunohistochemistry-Paraffin-NBP3-12158-img0006.jpg)
![Flow Cytometry: JWA Antibody [NBP3-12158] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/JWA-Antibody-Flow-Cytometry-NBP3-12158-img0004.jpg)
![Western Blot: JWA Antibody [NBP3-12158] - Staining of Human Cerebellum lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JWA-Antibody-Western-Blot-NBP3-12158-img0001.jpg)
![Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Tonsil with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/JWA-Antibody-Immunohistochemistry-Paraffin-NBP3-12158-img0005.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67373-img0006.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded human kidney tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0004.jpg)
![Western Blot: TPX2 Antibody (TPX2-01) [NBP2-67265] - Analysis of TPX2 using monoclonal antibody TPX2 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.](http://images.novusbio.com/fullsize/TPX2-Antibody-TPX2-01-Western-Blot-NBP2-67265-img0001.jpg "Western Blot: TPX2 Antibody (TPX2-01) [NBP2-67265] - Analysis of TPX2 using monoclonal antibody TPX2 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.")
![Immunocytochemistry: TPX2 Antibody (TPX2-01) [NBP2-67265] - Glioblastoma cell line T98G: a) interphase, b) mitosis. Bar: 20 um.](http://images.novusbio.com/fullsize/TPX2-Antibody-TPX2-01-Immunocytochemistry-NBP2-67265-img0003.jpg)
![Immunohistochemistry: TPX2 Antibody (TPX2-01) [NBP2-67265] - TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 um.](http://images.novusbio.com/fullsize/TPX2-Antibody-TPX2-01-Immunohistochemistry-NBP2-67265-img0004.jpg)
![Immunoprecipitation: TPX2 Antibody (TPX2-01) [NBP2-67265] - TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2. 1) nuclear extract, 2) antibody, 3) immunoprecipitate, 4) carrier](http://images.novusbio.com/fullsize/TPX2-Antibody-TPX2-01-Immunoprecipitation-NBP2-67265-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![<b>Biological Strategies Validation. </b>ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)