| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74040-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38103-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60241-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03908-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05384-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35743-20ul | |||
![Western Blot: CtBP1 Antibody (4D6) [NBP1-74040] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/CtBP1-Antibody-4D6-Western-Blot-NBP1-74040-img0001.jpg "Western Blot: CtBP1 Antibody (4D6) [NBP1-74040] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: CtBP1 Antibody (4D6) [NBP1-74040] - The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: NIH3T3 cell lysate Lane 3.: 293T cell lysate Lane 4.: AGS cell lysateLane 5.: Raji cell lysateLane 6.: A549 cell lysateLane 7.: Jurkat cell lysate](http://images.novusbio.com/fullsize/CtBP1-Antibody-4D6-Western-Blot-NBP1-74040-img0002.jpg)
![Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286] - Western blot analysis of extracts of various cell lines, using Rad50 Rabbit mAb (NBP3-16286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Rad50-Antibody-5M2K10-Western-Blot-NBP3-16286-img0001.jpg "Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286] - Western blot analysis of extracts of various cell lines, using Rad50 Rabbit mAb (NBP3-16286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunoprecipitation: Rad50 Antibody (5M2K10) [NBP3-16286] - Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug Rad50 antibody (NBP3-16286). Western blot was performed from the immunoprecipitate using Rad50 antibody (NBP3-16286) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Rad50-Antibody-5M2K10-Immunoprecipitation-NBP3-16286-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: NCAPH Antibody [NBP3-38103] - Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38103_rabbit-ncaph-pab-912202415161866.jpg "Immunocytochemistry/ Immunofluorescence: NCAPH Antibody [NBP3-38103] - Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: NCAPH Antibody [NBP3-38103] - Western blot analysis of various lysates using NCAPH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38103_rabbit-ncaph-pab-91220241418134.jpg)
![Western Blot: CHAF1B Antibody (7N0I2) [NBP3-16545] - Western blot analysis of extracts of various cell lines, using CHAF1B Rabbit mAb (NBP3-16545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-7N0I2-Western-Blot-NBP3-16545-img0002.jpg "Western Blot: CHAF1B Antibody (7N0I2) [NBP3-16545] - Western blot analysis of extracts of various cell lines, using CHAF1B Rabbit mAb (NBP3-16545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CHAF1B Antibody (7N0I2) [NBP3-16545] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CHAF1B Rabbit mAb (NBP3-16545) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-7N0I2-Immunohistochemistry-Paraffin-NBP3-16545-img0001.jpg)
![Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CtBP1-Antibody-1I7G6-Western-Blot-NBP3-15412-img0001.jpg "Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/CtBP1-Antibody-1I7G6-Western-Blot-NBP3-15412-img0002.jpg)
![Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Chk1-Antibody-0S3Q1-Western-Blot-NBP3-16353-img0002.jpg "Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Chk1 Antibody (0S3Q1) [NBP3-16353] - Immunohistochemistry of paraffin-embedded human colon using Chk1 Rabbit mAb (NBP3-16353) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Chk1-Antibody-0S3Q1-Immunohistochemistry-Paraffin-NBP3-16353-img0001.jpg)
![Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-2E3-Western-Blot-NBP2-60241-img0001.jpg "Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate")
![Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-2E3-Immunocytochemistry-Immunofluorescence-NBP2-60241-img0002.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Western Blot: HMGA1 Antibody [NBP3-03908] - Analysis of extracts of 293T cells, using HMGA1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/fullsize/HMGA1-Antibody-Western-Blot-NBP3-03908-img0003.jpg "Western Blot: HMGA1 Antibody [NBP3-03908] - Analysis of extracts of 293T cells, using HMGA1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.")
![Immunohistochemistry-Paraffin: HMGA1 Antibody [NBP3-03908] - Paraffin-embedded human stomach using HMGA1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HMGA1-Antibody-Immunohistochemistry-Paraffin-NBP3-03908-img0002.jpg)
![Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MDM2-HDM2-[p-Ser166]-Antibody-Western-Blot-NBP3-05384-img0002.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 [p Ser166] Antibody - BSA Free [MDM2/HDM2] - Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using MDM2/HDM2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-05384_rabbit-polyclonal-mdm2-hdm2-p-ser166-antibody-5220251640233.jpg)
![Western Blot: HMGB2 Antibody [NBP3-35743] - Western blot analysis of various lysates using HMGB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35743_rabbit-hmgb2-pab-912202415171171.jpg "Western Blot: HMGB2 Antibody [NBP3-35743] - Western blot analysis of various lysates using HMGB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Western-Blot-NBL1-13748-img0003.jpg "Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.")
![Western Blot: Nucleophosmin Overexpression Lysate (Adult Normal) [NBL1-13748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleophosmin.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13748-img0002.jpg)