| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26493-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26247-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26406-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34502-0.1mg | |||
![Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0059.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM3 Antibody [NB100-249] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-249-img0061.jpg)
![Western Blot: MCM3 Antibody [NB100-249] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0002.jpg)
![Western Blot: NOX5 Antibody [NBP3-42355] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-42355_rabbit-nox5-pab-612202421262948.jpg "Western Blot: NOX5 Antibody [NBP3-42355] - Sample: Recombinant protein.")
![Immunohistochemistry-Paraffin: NOX5 Antibody [NBP3-42355] - DAB staining on fromalin fixed paraffin-embedded Kidney tissue](http://images.novusbio.com/fullsize/nbp3-42355_rabbit-nox5-pab-612202421243242.jpg)
![Immunohistochemistry-Paraffin: NOX5 Antibody [NBP3-42355] - DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 10ug/ml NBP3-42355; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42355_rabbit-nox5-pab-61220242050363.jpg)
![Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug). Lane 3: PC3 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-262202413221524.jpg "Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug). Lane 3: PC3 whole cell lysate (20ug).")
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-26220241547561.jpg)
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-2722024845325.jpg)
![Western Blot: ATM Antibody (4E11) [NBP3-26493] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate. All lanes: ATM Antibody at 2.05ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 350 KDa Observed band size: 350 KDa](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-28220241140928.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202411351616.jpg "Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Staining of PC-3 cell with 14-3-3 beta/alpha Antibody (6F6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202412493639.jpg)
![Flow Cytometry: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Overlay Peak curve showing Hela cells stained with 14-3-3 beta/alpha Antibody (6F6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202414183655.jpg)
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-2622024152248.jpg "Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate. All lanes: TOR/mTOR [p Ser2448] Antibody at 0.825ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241140938.jpg)
![Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Staining of Hela cells with TOR/mTOR [p Ser2448] Antibody (1G6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241312233.jpg)
![Western Blot: Thyroid receptor-interacting protein 12 Antibody [NB100-97822] - Detection of human TRIP12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP12 antibody NB100-97822 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Western-Blot-NB100-97822-img0002.jpg "Western Blot: Thyroid receptor-interacting protein 12 Antibody [NB100-97822] - Detection of human TRIP12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP12 antibody NB100-97822 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: Thyroid receptor-interacting protein 12 Antibody [NB100-97822] - Detection of human TRIP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP12 antibody A301-814A (lot A301-814A-2) used for IP at 6 ug per reaction. TRIP12 was also immunoprecipitated by a previous lot of this antibody (lot A301-814A-1). For blotting immunoprecipitated TRIP12, A301-814A was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunoprecipitation-NB100-97822-img0003.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TS106) [NBP2-34445] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TS106).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-TS106-Immunohistochemistry-Paraffin-NBP2-34445-img0002.jpg "Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TS106) [NBP2-34445] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TS106).")
![Western Blot: MAPRE1 Antibody (C5) [NBP3-41927] - Sample: Lane1: Rat Thymus lysate; Lane2: Hela cell lysate; Lane3: Jurkat cell lysate Primary Ab: 3ug/ml NBP3-41927; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41927_mouse-mapre1-mab-c5-612202421195649.jpg "Western Blot: MAPRE1 Antibody (C5) [NBP3-41927] - Sample: Lane1: Rat Thymus lysate; Lane2: Hela cell lysate; Lane3: Jurkat cell lysate Primary Ab: 3ug/ml NBP3-41927; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: MAPRE1 Antibody (C5) [NBP3-41927] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 30ug/ml NBP3-41927; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41927_mouse-mapre1-mab-c5-612202421373059.jpg)
![Immunohistochemistry-Paraffin: MAPRE1 Antibody (C5) [NBP3-41927] - DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 30ug/ml NBP3-41927; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41927_mouse-mapre1-mab-c5-612202421175918.jpg)
![Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0066.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM6 Antibody [NB100-251] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunohistochemistry-NB100-251-img0068.jpg)
![Western Blot: MCM6 Antibody [NB100-251] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0002.jpg)
![Western Blot: Orc2 Antibody [NBP1-46176] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46176 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46175, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Orc2-Antibody-Western-Blot-NBP1-46176-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Orc2 Antibody [NBP1-46176] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46176 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46175, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Orc2 Antibody [NBP1-46176] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:200 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/Orc2-Antibody-Immunohistochemistry-NBP1-46176-img0003.jpg)
![Western Blot: BIN1 Antibody (99D) [NBP2-21673] - Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 ug per lane. BIN1 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band: non-specific.](http://images.novusbio.com/fullsize/BIN1-Antibody-99D-Western-Blot-NBP2-21673-img0001.jpg "Western Blot: BIN1 Antibody (99D) [NBP2-21673] - Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 ug per lane. BIN1 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band: non-specific.")
