| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98826-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50172-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81058-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20131-0.025mg | |||
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)
![Western Blot: TLK1 Antibody [NBP2-98826] - Lane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:87 kDaObserved band size:87 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/TLK1-Antibody-Western-Blot-NBP2-98826-img0003.jpg "Western Blot: TLK1 Antibody [NBP2-98826] - Lane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:87 kDaObserved band size:87 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: TLK1 Antibody [NBP2-98826] - Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate4 uL anti-TLK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TLK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 87 kDaObserved band size: 87 kDa](http://images.novusbio.com/fullsize/TLK1-Antibody-Immunoprecipitation-NBP2-98826-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TLK1 Antibody [NBP2-98826] - Immunofluorescence staining of TLK1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98826 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98826_rabbit-polyclonal-tlk1-antibody-235202511354115.png)
![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)
![Immunocytochemistry/ Immunofluorescence: SON Antibody - BSA Free [NBP3-30739] - Immunofluorescence staining of SON in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-30739 (dilution ratio 1:100) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-30739_rabbit-son-pab-235202511545686.png "Immunocytochemistry/ Immunofluorescence: SON Antibody - BSA Free [NBP3-30739] - Immunofluorescence staining of SON in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-30739 (dilution ratio 1:100) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: SON Antibody - BSA Free [NBP3-30739] - Immunochemical staining of human SON in human prostate with NBP3-30739 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-30739_rabbit-son-pab-235202514492369.png)
![Immunohistochemistry-Paraffin: SON Antibody - BSA Free [NBP3-30739] - Immunochemical staining of human SON in human small intestine with NBP3-30739 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-30739_rabbit-son-pab-235202514371322.png)
![Adhesion Activation: HGF R/c-MET Antibody (8) [NBP2-50172] - Epitope position on molecule](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-8-Adhesion-Activation-NBP2-50172-img0001.jpg "Adhesion Activation: HGF R/c-MET Antibody (8) [NBP2-50172] - Epitope position on molecule")
![Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.](http://images.novusbio.com/fullsize/nbp3-23294_rat-eb3-mab-kt36-11920231633169.jpg "Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.")
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (KT36) [NBP3-23294] - Immunocytochemical labeling of EB3 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (green) and rat monoclonal anti-EB3 (NBP3-23294) (red). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat:DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23294_rat-eb3-mab-kt36-19920231856937.jpg)
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M138) [NBP3-23233] - Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23233_mouse-p38-alpha-mab-m138-19920231410114.jpg "Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M138) [NBP3-23233] - Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.")
![Western Blot: p38 alpha Antibody (M138) [NBP3-23233] - Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38 alpha (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).](http://images.novusbio.com/fullsize/nbp3-23233_mouse-p38-alpha-mab-m138-19920231252360.jpg)
![Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.](http://images.novusbio.com/fullsize/Septin-5-Antibody-SP18-Western-Blot-NBP2-81058-img0001.jpg "Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.")
![Immunohistochemistry: Septin-5 Antibody (SP18) [NBP2-81058] - Immunohistochemical staining of rabbit brain using [NBP2-81058]. Formalin fixed rabbit brain slices were were stained with NBP2-81058 at 3 ug/ml.](http://images.novusbio.com/fullsize/Septin-5-Antibody-SP18-Immunohistochemistry-NBP2-81058-img0002.jpg)
![Western Blot: EB2 Antibody (KT52) [NBP3-23289] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.](http://images.novusbio.com/fullsize/nbp3-23289_rat-eb2-mab-kt52-199202312523645.jpg "Western Blot: EB2 Antibody (KT52) [NBP3-23289] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.")
![Immunocytochemistry/Immunofluorescence: EB2 Antibody (KT52) [NBP3-23289] - Immunocytochemical labeling of EB2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-EB2 (NBP3-23289) (right). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23289_rat-eb2-mab-kt52-19920231856947.jpg)
![Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20131_rabbit-prmt5-mab-rab-c136-chimeric-azide-and-bsa-free-2742023924283.png "Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C136 (NBP3-20131) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20131, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20131_rabbit-prmt5-mab-rab-c136-chimeric-azide-and-bsa-free-2742023924232.png)
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M548) [NBP3-23234] - Immunocytochemical labeling of p38 alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38 alpha (NBP3-23234). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23234_mouse-p38-alpha-mab-m548-199202318202923.jpg "Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M548) [NBP3-23234] - Immunocytochemical labeling of p38 alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38 alpha (NBP3-23234). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Western Blot: p38 alpha Antibody (M548) [NBP3-23234] - Western blot analysis of human A431 cells (lanes 1 & 2) and rat A7r5 cells (lanes 3 & 4). The blot was probed with mouse monoclonal anti-p38 alpha (C-terminal) clone M548 at a dilution of 1:250 (lanes 1 & 3) or 1:1000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23234_mouse-p38-alpha-mab-m548-19920231856916.jpg)
![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody (SPM312) [NBP2-54475] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with PSAP Monoclonal Antibody (SPM312).](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-SPM312-Immunohistochemistry-Paraffin-NBP2-54475-img0001.jpg "Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody (SPM312) [NBP2-54475] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with PSAP Monoclonal Antibody (SPM312).")