Septin-5 Antibody (SP18) - Azide and BSA Free

Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.

Immunohistochemistry: Septin-5 Antibody (SP18) [NBP2-81058] - Immunohistochemical staining of rabbit brain using [NBP2-81058]. Formalin fixed rabbit brain slices were were stained with NBP2-81058 at 3 ug/ml.

• Reactivity: Hu, Rt, Rb
• Applications: WB, ELISA, IHC
• Clone: SP18
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Septin-5 Antibody (SP18) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Septin-5 Antibody (SP18) - Azide and BSA Free (NBP2-81058) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Immunoprecipitate from human brain.
• Specificity: This antibody recognises Septin-5 (CDCrel-1) and does not cross-react with CDCrel-2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEPTIN5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Septin-5 Antibody (SP18) - Azide and BSA Free

• CDCREL
• CDCREL1
• CDCREL-1
• cell division control related protein 1
• Cell division control-related protein 1
• HCDCREL-1
• peanut-like 1 (Drosophila)
• peanut-like 1
• Peanut-like protein 1
• platelet glycoprotein Ib beta chain
• PNUTL1H5
• septin 5
• septin-5

Background

This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Two transcripts of this gene, a major one of 2.2 kb and a minor one of 3.5 kb, have been observed. The 2.2 kb form results from the utilization of a non-consensus polyA signal (AACAAT). In the absence of polyadenylation from this imperfect site, the consensus polyA signal of the downstream neighboring gene (GP1BB; platelet glycoprotein Ib) is used, resulting in the 3.5 kb transcript. An alternatively spliced transcript variant with a different 5' end has also been identified, but its full-length nature has not been completely determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEPTIN5