| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05342-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72566 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03303-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2646-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98433-50ul | |||
![Western Blot: pan Cadherin Antibody (2M6G8) [NBP3-05342] - analysis of extracts of various cell lines, using E-Cadherin antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/antibody/nbp3-05342_mouse-polyclonal-pan-cadherin-antibody-amc0108-western-blot-199202311401..jpg "Western Blot: pan Cadherin Antibody (2M6G8) [NBP3-05342] - analysis of extracts of various cell lines, using E-Cadherin antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![ICC/IF-pan Cadherin Antibody (2M6G8) [NBP3-05342] - Analysis of mouse kidney cells using E-Cadherin Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-05342_mouse-polyclonal-pan-cadherin-antibody-amc0108-immunocytochemistry-immunofluorescence-1992023114216..jpg)
![Western Blot: MAD2L1-binding protein Antibody (3C11) [NBP2-45705] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAD2L1- binding protein.](http://images.novusbio.com/fullsize/MAD2L1-binding-protein-Antibody-3C11-Western-Blot-NBP2-45705-img0002.jpg "Western Blot: MAD2L1-binding protein Antibody (3C11) [NBP2-45705] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAD2L1- binding protein.")
![Immunohistochemistry: MAD2L1-binding protein Antibody (3C11) [NBP2-45705] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MAD2L1-binding-protein-Antibody-3C11-Immunohistochemistry-NBP2-45705-img0001.jpg)
![Western Blot: UbcH10/UBE2C Antibody [NBP3-03303] - Analysis of extracts from normal (control) and UBE2C knockout (KO) HeLa cells, using UbcH10/UBE2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Knockout-Validated-NBP3-03303-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: UbcH10/UBE2C Antibody [NBP3-03303] - Analysis of extracts from normal (control) and UBE2C knockout (KO) HeLa cells, using UbcH10/UBE2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Western blot analysis of extracts of various cell lines, using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-5G5L9-Western-Blot-NBP3-16588-img0002.jpg "Western Blot: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Western blot analysis of extracts of various cell lines, using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Immunofluorescence analysis of U-2 OS cells using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-5G5L9-Immunocytochemistry-Immunofluorescence-NBP3-16588-img0001.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98433-img0002.jpg "Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody [NBP2-98433] - Immunofluorescence staining of E-Cadherin in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98433 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98433_rabbit-polyclonal-e-cadherin-antibody-23520252135138.png)
![Western Blot: Apc2 Antibody [NBP2-87013] - WB Suggested Anti-APC2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta](http://images.novusbio.com/fullsize/Apc2-Antibody-Western-Blot-NBP2-87013-img0002.jpg "Western Blot: Apc2 Antibody [NBP2-87013] - WB Suggested Anti-APC2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta")
![Immunohistochemistry: Apc2 Antibody [NBP2-87013] - Skin](http://images.novusbio.com/fullsize/Apc2-Antibody-Immunohistochemistry-NBP2-87013-img0001.jpg)
![Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-27820241628529.jpg "Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32289) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-298202413585533.jpg)
![Western Blot: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Analysis of FZR1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-3E12-Western-Blot-H00051343-M09-img0003.jpg "Western Blot: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Analysis of FZR1 expression in HeLa (Cat # L013V1).")
![Sandwich ELISA: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Detection limit for recombinant GST tagged FZR1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-3E12-Sandwich-ELISA-H00051343-M09-img0002.jpg)
![Western Blot: Cdc27 Antibody [NBP2-15829] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC27 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Cdc27-Antibody-Western-Blot-NBP2-15829-img0002.jpg "Western Blot: Cdc27 Antibody [NBP2-15829] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC27 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
