| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76298-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35401-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03535-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05379-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97936-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98309-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NBP1-31098-img0008.jpg "Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KPNA2 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-NBP1-31098-img0011.jpg)
![karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term (NBP1-31098) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-31098_rabbit-polyclonal-importin-alpha-2-kpna2-antibody-immunocytochemistry-immunofluorescence-141120251844345.jpg)
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Western Blot: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Western-Blot-NBP3-13705-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Immunocytochemistry-Immunofluorescence-NBP3-13705-img0003.jpg)
![Immunohistochemistry-Paraffin: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Immunohistochemistry-Paraffin-NBP3-13705-img0002.jpg)
![Untreated (-) and treated (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13705_rabbit-atm-p-ser-1981-mab-hl1062-western-blot-261120251845135.jpg)
![Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13705_rabbit-atm-p-ser-1981-mab-hl1062-western-blot-261120251836813.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0005.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.")
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Rat heart tissue lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0001.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Immunohistochemistry of KPNA2 in human heart tissue with KPNA2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NBP1-76298-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody - BSA Free [NBP1-76298] - Immunofluorescence of Importin alpha 2/KPNA2 in human heart tissue with Importin alpha 2/KPNA2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76298_rabbit-polyclonal-importin-alpha-2-kpna2-antibody-2711202419432251.jpg)
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat heart using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241519590.jpg "Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat heart using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241513346.jpg)
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat liver using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241418155.jpg)
![Western Blot: HUS1 Antibody [NBP3-35401] - Western blot analysis of lysates from mouse kidney, using HUS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-912202414213762.jpg)
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-912202414163053.jpg)
![Immunocytochemistry/ Immunofluorescence: PIDD Antibody - BSA Free [PIDD] - Immunofluorescence analysis of HeLa cells using PIDD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03535_rabbit-polyclonal-lrdd-antibody-52202516334114.jpg "Immunocytochemistry/ Immunofluorescence: PIDD Antibody - BSA Free [PIDD] - Immunofluorescence analysis of HeLa cells using PIDD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded mouse brain using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0004.jpg)
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded human lung cancer using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0002.jpg)
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded rat brain using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0003.jpg)
![Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Western-Blot-NBP3-05379-img0005.jpg "Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded mouse brain using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded rat heart using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0001.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded human placenta using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0002.jpg)
![Western Blot: Chk1 [p Ser280] Antibody - Azide and BSA Free [Chk1] - Western blot analysis of lysates from 293T cells, using Chk1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-05379_rabbit-polyclonal-chk1-p-ser280-antibody-52202516293974.jpg)
![Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-27820241631628.jpg "Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32173) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29820241420958.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunocytochemistry analysis of HeLa cells labeling CDC25C with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-2920241023428.jpg)
![Flow Cytometry: CDC25C Antibody (JE59-53) [NBP3-32173] - Flow cytometric analysis of HeLa cells labeling CDC25C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32173, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29202416453230.jpg)
![Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-97936-img0005.jpg "Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-97936] - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97936-img0004.jpg)
![Immunoprecipitation: RFC4 Antibody [NBP2-97936] - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunoprecipitation-NBP2-97936-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-97936] - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97936 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97936_rabbit-polyclonal-rfc4-antibody-23520251664787.png)
![Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-278202416331255.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29820241426265.jpg)
![Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunocytochemistry analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A with Rabbit anti-p21/CIP1/CDKN1A/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202410334083.jpg)
![Flow Cytometry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Flow cytometric analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32691, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202416372178.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-98309-img0006.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunochemical staining of mouse 14-3-3 sigma/Stratifin in mouse skin with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP2-98309-img0004.jpg)
![Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Mouse 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 ul anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunoprecipitation-NBP2-98309-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98309 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98309_rabbit-polyclonal-14-3-3-sigma-stratifin-antibody-23520252135137.png)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)